Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Angelicin synthase

Gene

CYP71AJ4

Organism
Pastinaca sativa (Parsnip) (Anethum pastinaca)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in angular furanocumarin biosynthesis. Converts (+)-columbianetin to angelicin. Produces also some hydroxycolumbianetin as a by-product. No activity with demethylsuberosin, (+)-marmesin, 5-hydroxymarmesin, psoralen, bergaptol, xanthotoxol, bergapten, xanthotoxin, isopimpinellin, cinnamic acid, 4-coumaric acid, 2-coumaric acid, ferulic acid, coumarin, herniarin, scopoletin, or umbelliferone. The reaction mechanism is initiated by the abstraction of syn-C-3'-hydrogen.1 Publication

Catalytic activityi

+-columbianetin + NADPH + O2 = angelicin + NADP+ + acetone + 2 H2O.1 Publication

Enzyme regulationi

Not inhibited by (+)-marmesin, psoralen, 5- or 8-methoxypsoralen and angelicin.1 Publication

Kineticsi

  1. KM=2.1 µM for (+)-columbianetin1 Publication
  2. KM=1.4 µM for syn-[3'-D]columbianetin1 Publication

    pH dependencei

    Optimum pH is 7.0.1 Publication

    Temperature dependencei

    Optimum temperature is 27-30 degrees Celsius.1 Publication

    GO - Molecular functioni

    Complete GO annotation...

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Heme, Iron, Metal-binding, NADP

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15618.
    BRENDAi1.14.13.115. 4562.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Angelicin synthase (EC:1.14.13.115)
    Alternative name(s):
    Cytochrome P450 CYP71AJ4
    Gene namesi
    Name:CYP71AJ4
    OrganismiPastinaca sativa (Parsnip) (Anethum pastinaca)
    Taxonomic identifieri4041 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridscampanulidsApialesApiaceaeApioideaeapioid supercladeTordylieaeTordyliinaePastinaca

    Subcellular locationi

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Transmembranei1 – 17HelicalBy similarityAdd BLAST17

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_0000401480‹1 – ›478Angelicin synthaseAdd BLAST›478

    Structurei

    3D structure databases

    ProteinModelPortaliC0SJS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the cytochrome P450 family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    C0SJS3-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    YFFSLFLVTI FLYKWLAKKT PSKNLPPSPP RLPIIGNLHQ IGPDLHISLR
    60 70 80 90 100
    DLARKYGPLM QLQLGRIPVL VVSSAEATRE VLKTHDVVFS QRPITSAIDK
    110 120 130 140 150
    LCYKGRDVAF SRYSEYWRQV RSTCVTQLLS NSRVHSFHNI REEEVALLIQ
    160 170 180 190 200
    NIENSASEVI NLGEQLIQLT RNVVCRVSVG SEYLSGHKGK LYQKLLAEVT
    210 220 230 240 250
    EMLAYTYSIG DFIPLLGWVD WLSGSKAKVE KTAKEVDAFL EGALRDHIKT
    260 270 280 290 300
    MASNKGSAND DFLSILLEIR EADAGSTLDE ECIKAIVWDM ILGGTETTST
    310 320 330 340 350
    TLEWIVAAII KNPDVMFKLQ KEVREIGKGK SKIEEVDLVK MNYLKAVMKE
    360 370 380 390 400
    SMRLYITAFL LPREAKQDVK LMGYDISSGT QVLINTWETA RDPSLWDNPE
    410 420 430 440 450
    EFRPERFLNS PIDYKGLHYE YLPFGGGRRG CPGIQFAMAV NELAVANVVY
    460 470
    KFDFKMPDGE RFEDLDMSGV PGISLYRK
    Length:478
    Mass (Da):54,135
    Last modified:May 5, 2009 - v1
    Checksum:i8ACB6CD76A0F6B04
    GO

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Non-terminal residuei11
    Non-terminal residuei4781

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF191021 mRNA. Translation: ABO84854.1.

    Genome annotation databases

    KEGGiag:ABO84854.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    EF191021 mRNA. Translation: ABO84854.1.

    3D structure databases

    ProteinModelPortaliC0SJS3.
    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    KEGGiag:ABO84854.

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-15618.
    BRENDAi1.14.13.115. 4562.

    Family and domain databases

    Gene3Di1.10.630.10. 1 hit.
    InterProiIPR001128. Cyt_P450.
    IPR017972. Cyt_P450_CS.
    IPR002401. Cyt_P450_E_grp-I.
    [Graphical view]
    PfamiPF00067. p450. 1 hit.
    [Graphical view]
    PRINTSiPR00463. EP450I.
    PR00385. P450.
    SUPFAMiSSF48264. SSF48264. 1 hit.
    PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
    [Graphical view]
    ProtoNetiSearch...

    Entry informationi

    Entry nameiANGS_PASSA
    AccessioniPrimary (citable) accession number: C0SJS3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 30, 2010
    Last sequence update: May 5, 2009
    Last modified: October 5, 2016
    This is version 27 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.