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C0RJN6 (C0RJN6_BRUMB) Unreviewed, UniProtKB/TrEMBL

Last modified February 19, 2014. Version 35. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase HAMAP-Rule MF_00047

EC=6.3.2.4 HAMAP-Rule MF_00047
Alternative name(s):
D-Ala-D-Ala ligase HAMAP-Rule MF_00047
D-alanylalanine synthetase HAMAP-Rule MF_00047
Gene names
Name:ddl HAMAP-Rule MF_00047
Ordered Locus Names:BMEA_A1317 EMBL ACO01044.1
OrganismBrucella melitensis biotype 2 (strain ATCC 23457) [Complete proteome] [HAMAP] EMBL ACO01044.1
Taxonomic identifier546272 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length353 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047 SAAS SAAS005905

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity. HAMAP-Rule MF_00047 SAAS SAAS005905

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047 SAAS SAAS005905

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047 SAAS SAAS005905.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family. HAMAP-Rule MF_00047

Contains 1 ATP-grasp domain. HAMAP-Rule MF_00047

Contains ATP-grasp domain. SAAS SAAS005905

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Regions

Domain141 – 346206ATP-grasp By similarity HAMAP-Rule MF_00047
Nucleotide binding169 – 22456ATP By similarity HAMAP-Rule MF_00047

Sites

Metal binding3001Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 1 By similarity HAMAP-Rule MF_00047
Metal binding3131Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047
Metal binding3151Magnesium or manganese 2 By similarity HAMAP-Rule MF_00047

Sequences

Sequence LengthMass (Da)Tools
C0RJN6 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 8CC9BFE58A2CFF2C

FASTA35337,530
        10         20         30         40         50         60 
MASGKMRIVV LFGGRSAEHD VSVLSATNVM NALDPAKYEA VPVFVTRAGQ WLLSRFVNGA 

        70         80         90        100        110        120 
LEKLSSGAEL CLVPGGCGRA IVVPDAGAPY EADKIDIIFP VLHGLHGEDG AVQGLAQVAR 

       130        140        150        160        170        180 
VPLAGCGIPG SANALDKDIA KRLVNEAGLS TAKSVTITRE EVPAFSALEQ ALGLPIFIKP 

       190        200        210        220        230        240 
ARQGSSVGVH KVVTEADYQA AMSDGFIYDD KLLAEEFIQA REVECGVLED EGGALFVSRA 

       250        260        270        280        290        300 
GEIVPAESHC FYSYDAKYID ADGTEIKVPA ELPEQVENEI RAIAAKAFRV LGCDSMARVD 

       310        320        330        340        350 
FFVTADRRIV LNEINTIPGF TDMSMYSKVM AVSGVSYPEI INRLVAHGLA RGS 

« Hide

References

[1]"Brucella melitensis ATCC 23457 whole genome shotgun sequencing project."
Setubal J.C., Boyle S., Crasta O.R., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Nagrani S., Shallom J.M., Shallom S., Shukla M., Snyder E.E., Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Munk C. expand/collapse author list , Tapia R., Han C., Detter J.C., Bruce D., Brettin T.S.
Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 23457.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001488 Genomic DNA. Translation: ACO01044.1.
RefSeqYP_002732998.1. NC_012441.1.

3D structure databases

ProteinModelPortalC0RJN6.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING546272.BMEA_A1317.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACO01044; ACO01044; BMEA_A1317.
GeneID7677044.
KEGGbmi:BMEA_A1317.
PATRIC17838389. VBIBruMel14466_1335.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011593.
KOK01921.
OMAMDKIAMK.
OrthoDBEOG64BQ73.
ProtClustDBPRK01966.

Enzyme and pathway databases

BioCycBMEL546272:GJOX-1280-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameC0RJN6_BRUMB
AccessionPrimary (citable) accession number: C0RJN6
Entry history
Integrated into UniProtKB/TrEMBL: May 5, 2009
Last sequence update: May 5, 2009
Last modified: February 19, 2014
This is version 35 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)