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C0RFF1

- LEUC_BRUMB

UniProt

C0RFF1 - LEUC_BRUMB

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Protein

3-isopropylmalate dehydratase large subunit

Gene

leuC

Organism
Brucella melitensis biotype 2 (strain ATCC 23457)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate.UniRule annotation

Catalytic activityi

(2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster per subunit.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi350 – 3501Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi410 – 4101Iron-sulfur (4Fe-4S)UniRule annotation
Metal bindingi413 – 4131Iron-sulfur (4Fe-4S)UniRule annotation

GO - Molecular functioni

  1. 3-isopropylmalate dehydratase activity Source: UniProtKB-HAMAP
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. leucine biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BioCyciBMEL546272:GJOX-1909-MONOMER.
UniPathwayiUPA00048; UER00071.

Names & Taxonomyi

Protein namesi
Recommended name:
3-isopropylmalate dehydratase large subunitUniRule annotation (EC:4.2.1.33UniRule annotation)
Alternative name(s):
Alpha-IPM isomeraseUniRule annotation
Short name:
IPMIUniRule annotation
Isopropylmalate isomeraseUniRule annotation
Gene namesi
Name:leuCUniRule annotation
Ordered Locus Names:BMEA_A1961
OrganismiBrucella melitensis biotype 2 (strain ATCC 23457)
Taxonomic identifieri546272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000001748: Chromosome I

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4694693-isopropylmalate dehydratase large subunitPRO_1000149356Add
BLAST

Interactioni

Subunit structurei

Heterodimer of LeuC and LeuD.UniRule annotation

Protein-protein interaction databases

STRINGi546272.BMEA_A1961.

Structurei

3D structure databases

ProteinModelPortaliC0RFF1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0065.
HOGENOMiHOG000226972.
KOiK01703.
OMAiQARAKTM.
OrthoDBiEOG600DP5.

Family and domain databases

Gene3Di3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPiMF_01026. LeuC_type1.
InterProiIPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view]
PANTHERiPTHR11670. PTHR11670. 1 hit.
PfamiPF00330. Aconitase. 1 hit.
[Graphical view]
PRINTSiPR00415. ACONITASE.
SUPFAMiSSF53732. SSF53732. 1 hit.
TIGRFAMsiTIGR00170. leuC. 1 hit.
PROSITEiPS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0RFF1-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSAPRTLYDK IWDDHVVDQQ EDGTCLLYID RHLVHEVTSP QAFEGLHMAG
60 70 80 90 100
RPVRHPEKTL AVVDHNVPTS PDRINGIQNE ESRIQVEALA RNAADFGVEY
110 120 130 140 150
YSERDKRQGI VHIVGPEQGF TLPGMTIVCG DSHTSTHGAF GALAHGIGTS
160 170 180 190 200
EVEHVLATQT LIQKKAKNML VRVDGKLPAG VTAKDIVLAI IGEIGTAGGT
210 220 230 240 250
GYVIEYAGEA IRSLSMEGRM TICNMSIEGG ARAGLIAPDE TTFEYIKGRP
260 270 280 290 300
RAPQGETLEQ AINYWKTLHS DEGAHFDKIV TLDAGSLPPI VSWGSSPEDV
310 320 330 340 350
VSVTGVVPNP DDIADETKRA SKWRALDYMG LKPGTKITDI AVDRVFIGSC
360 370 380 390 400
TNGRIEDLRA AAKVVEGKKV APTVNAMIVP GSGLVKEQAE AEGLHKIFIE
410 420 430 440 450
AGFDWREPGC SMCLAMNDDR LKPGERCAST SNRNFEGRQG FKGRTHLVSP
460
AMAAAAAIAG HFVDIRAWK
Length:469
Mass (Da):50,692
Last modified:May 5, 2009 - v1
Checksum:i14442D949C40BCDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001488 Genomic DNA. Translation: ACO01623.1.
RefSeqiYP_002733577.1. NC_012441.1.

Genome annotation databases

EnsemblBacteriaiACO01623; ACO01623; BMEA_A1961.
GeneIDi7677608.
KEGGibmi:BMEA_A1961.
PATRICi17839718. VBIBruMel14466_1976.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001488 Genomic DNA. Translation: ACO01623.1 .
RefSeqi YP_002733577.1. NC_012441.1.

3D structure databases

ProteinModelPortali C0RFF1.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 546272.BMEA_A1961.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO01623 ; ACO01623 ; BMEA_A1961 .
GeneIDi 7677608.
KEGGi bmi:BMEA_A1961.
PATRICi 17839718. VBIBruMel14466_1976.

Phylogenomic databases

eggNOGi COG0065.
HOGENOMi HOG000226972.
KOi K01703.
OMAi QARAKTM.
OrthoDBi EOG600DP5.

Enzyme and pathway databases

UniPathwayi UPA00048 ; UER00071 .
BioCyci BMEL546272:GJOX-1909-MONOMER.

Miscellaneous databases

PROi C0RFF1.

Family and domain databases

Gene3Di 3.30.499.10. 3 hits.
3.40.1060.10. 1 hit.
HAMAPi MF_01026. LeuC_type1.
InterProi IPR004430. 3-IsopropMal_deHydase_lsu.
IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3.
IPR015937. Acoase/IPM_deHydtase.
IPR001030. Acoase/IPM_deHydtase_lsu_aba.
IPR015932. Aconitase/IPMdHydase_lsu_aba_2.
IPR018136. Aconitase_4Fe-4S_BS.
[Graphical view ]
PANTHERi PTHR11670. PTHR11670. 1 hit.
Pfami PF00330. Aconitase. 1 hit.
[Graphical view ]
PRINTSi PR00415. ACONITASE.
SUPFAMi SSF53732. SSF53732. 1 hit.
TIGRFAMsi TIGR00170. leuC. 1 hit.
PROSITEi PS00450. ACONITASE_1. 1 hit.
PS01244. ACONITASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Brucella melitensis ATCC 23457 whole genome shotgun sequencing project."
    Setubal J.C., Boyle S., Crasta O.R., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Nagrani S., Shallom J.M., Shallom S., Shukla M., Snyder E.E., Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Munk C.
    , Tapia R., Han C., Detter J.C., Bruce D., Brettin T.S.
    Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 23457.

Entry informationi

Entry nameiLEUC_BRUMB
AccessioniPrimary (citable) accession number: C0RFF1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: October 1, 2014
This is version 39 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3