C0REU2 (DUT_BRUMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

Name:	dut
Ordered Locus Names:	BMEA_A1728

Organism

Brucella melitensis biotype 2 (strain ATCC 23457) [Complete proteome] [HAMAP]

Taxonomic identifier

546272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	157 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA By similarity. HAMAP MF_00116
Catalytic activity	dUTP + H2O = dUMP + diphosphate. HAMAP MF_00116
Cofactor	Magnesium By similarity. HAMAP MF_00116
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 2/2. HAMAP MF_00116
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	dUTP metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 157	157	Deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP MF_00116		PRO_1000119227
Regions
	Region	76 – 78	3	Substrate binding By similarity
	Region	93 – 95	3	Substrate binding By similarity
Sites
	Binding site	89	1	Substrate By similarity
	Binding site	103	1	Substrate; via amide nitrogen and carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0REU2 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 7C9BB78942CFADEF	FASTA	157	16,660
	10 20 30 40 50 60 MTAASSSAPT LGIIRLEHAK GLDLPAYETA GSAGMDLRAA VAEDRQIVLL PGRRTLVPTG 70 80 90 100 110 120 LILEIPQGYE VQIRPRSGLA FKNGITCLNT PGTIDSDYRG EVKVLLINLG DDDFRIERGM 130 140 150 RIAQAVFAPV IQPKIEERAK ISETARGAGG FGSTGTA

References

[1]

"Brucella melitensis ATCC 23457 whole genome shotgun sequencing project." Setubal J.C., Boyle S., Crasta O.R., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Nagrani S., Shallom J.M., Shallom S., Shukla M., Snyder E.E., Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Munk C. , Tapia R., Han C., Detter J.C., Bruce D., Brettin T.S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23457.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001488 Genomic DNA. Translation: ACO01414.1.
RefSeq	YP_002733368.1. NC_012441.1.
3D structure databases
ProteinModelPortal	C0REU2.
ModBase	Search...
Protein-protein interaction databases
STRING	C0REU2.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7676637.
GenomeReviews	Gene locus BMEA_A1728 in contig CP001488_GR.
KEGG	bmi:BMEA_A1728.
PATRIC	17839238. VBIBruMel14466_1744.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	LDLRACI.
ProtClustDB	PRK00601.
Family and domain databases
HAMAP	MF_00116. dUTPase_bact. [Tree]
InterPro	IPR008180. dUTP_pyroPase. IPR008181. dUTP_pyroPase_sf. [Graphical view]
KO	K01520.
PANTHER	PTHR11241. PTHR11241. 1 hit.
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00576. Dut. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_BRUMB

Accession

Primary (citable) accession number: C0REU2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 5, 2009
Last modified:	January 25, 2012
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program