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Protein

Oxygen-dependent coproporphyrinogen-III oxidase

Gene

hemF

Organism
Brucella melitensis biotype 2 (strain ATCC 23457)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the heme biosynthesis. Catalyzes the aerobic oxidative decarboxylation of propionate groups of rings A and B of coproporphyrinogen-III to yield the vinyl groups in protoporphyrinogen-IX.UniRule annotation

Catalytic activityi

Coproporphyrinogen-III + O2 + 2 H+ = protoporphyrinogen-IX + 2 CO2 + 2 H2O.UniRule annotation

Cofactori

a divalent metal cationUniRule annotation

Pathwayi: protoporphyrin-IX biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Oxygen-dependent coproporphyrinogen-III oxidase (hemF)
This subpathway is part of the pathway protoporphyrin-IX biosynthesis, which is itself part of Porphyrin-containing compound metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protoporphyrinogen-IX from coproporphyrinogen-III (O2 route), the pathway protoporphyrin-IX biosynthesis and in Porphyrin-containing compound metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei116SubstrateUniRule annotation1
Metal bindingi120Divalent metal cationUniRule annotation1
Active sitei130Proton donorUniRule annotation1
Metal bindingi130Divalent metal cationUniRule annotation1
Metal bindingi168Divalent metal cationUniRule annotation1
Metal bindingi199Divalent metal cationUniRule annotation1
Sitei199Important for dimerizationUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processHeme biosynthesis, Porphyrin biosynthesis
LigandMetal-binding

Enzyme and pathway databases

UniPathwayiUPA00251; UER00322.

Names & Taxonomyi

Protein namesi
Recommended name:
Oxygen-dependent coproporphyrinogen-III oxidaseUniRule annotation (EC:1.3.3.3UniRule annotation)
Short name:
CPOUniRule annotation
Short name:
Coprogen oxidaseUniRule annotation
Short name:
CoproporphyrinogenaseUniRule annotation
Gene namesi
Name:hemFUniRule annotation
Ordered Locus Names:BMEA_A1605
OrganismiBrucella melitensis biotype 2 (strain ATCC 23457)
Taxonomic identifieri546272 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000001748 Componenti: Chromosome I

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001331751 – 303Oxygen-dependent coproporphyrinogen-III oxidaseAdd BLAST303

Interactioni

Subunit structurei

Homodimer.UniRule annotation

GO - Molecular functioni

Structurei

3D structure databases

ProteinModelPortaliC0REI6.
SMRiC0REI6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni132 – 134Substrate bindingUniRule annotation3
Regioni268 – 303Important for dimerizationUniRule annotationAdd BLAST36
Regioni286 – 288Substrate bindingUniRule annotation3

Sequence similaritiesi

Belongs to the aerobic coproporphyrinogen-III oxidase family.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000262768.
KOiK00228.
OMAiMDLTPYY.

Family and domain databases

Gene3Di3.40.1500.10. 1 hit.
HAMAPiMF_00333. Coprogen_oxidas. 1 hit.
InterProiView protein in InterPro
IPR001260. Coprogen_oxidase_aer.
IPR018375. Coprogen_oxidase_CS.
PANTHERiPTHR10755. PTHR10755. 1 hit.
PfamiView protein in Pfam
PF01218. Coprogen_oxidas. 1 hit.
PIRSFiPIRSF000166. Coproporphyri_ox. 1 hit.
PRINTSiPR00073. COPRGNOXDASE.
SUPFAMiSSF102886. SSF102886. 1 hit.
PROSITEiView protein in PROSITE
PS01021. COPROGEN_OXIDASE. 1 hit.

Sequencei

Sequence statusi: Complete.

C0REI6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKREDIPAII PADIEEKKKA AQSWFEELRD RICASYEQLE DELQGPLSDR
60 70 80 90 100
EPGRFVRTPW QKDDGNGGGV MSIMHGRVFE KVGVHVSTVH GEFSPEFRKQ
110 120 130 140 150
IPGAEEDPRY WASGISLIAH PQNPNVPAVH MNTRMIVTTR QWFAGGADLT
160 170 180 190 200
PVLDRRRTQE DPDTLAFHKA FRFICEKHKD IVDYQRLKEW CDEYFFLPHR
210 220 230 240 250
DEPRGIGGIF YDWLHSPEEK GGWDSDFAFT RDVGRGFSVV YPHLVRQNFN
260 270 280 290 300
KDWTEADRDE QLIRRGRYVE FNLLYDRGTI FGLKTGGNMN AILSSMPPVV

KWP
Length:303
Mass (Da):35,111
Last modified:May 5, 2009 - v1
Checksum:i65C4C1B754D13CE0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001488 Genomic DNA. Translation: ACO01308.1.
RefSeqiWP_002964654.1. NC_012441.1.

Genome annotation databases

EnsemblBacteriaiACO01308; ACO01308; BMEA_A1605.
GeneIDi29593250.
KEGGibmi:BMEA_A1605.

Similar proteinsi

Entry informationi

Entry nameiHEM6_BRUMB
AccessioniPrimary (citable) accession number: C0REI6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: September 27, 2017
This is version 52 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families