C0RE20 (ILVC_BRUMB) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ketol-acid reductoisomerase
EC=1.1.1.86

Alternative name(s):
Acetohydroxy-acid isomeroreductase
Alpha-keto-beta-hydroxylacil reductoisomerase

Gene names

Name:	ilvC
Ordered Locus Names:	BMEA_A1427

Organism

Brucella melitensis biotype 2 (strain ATCC 23457) [Complete proteome] [HAMAP]

Taxonomic identifier

546272 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	339 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH. HAMAP MF_00435 (2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH. HAMAP MF_00435
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 2/4. HAMAP MF_00435 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 2/4. HAMAP MF_00435
Sequence similarities	Belongs to the ketol-acid reductoisomerase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	coenzyme binding Inferred from electronic annotation. Source: InterPro ketol-acid reductoisomerase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 339	339	Ketol-acid reductoisomerase HAMAP MF_00435		PRO_1000190916
Sites
	Active site	108	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	C0RE20 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 1A4967D9205EEE92	FASTA	339	37,080
	10 20 30 40 50 60 MRVYYDRDAD VNLIKSKKVV IVGYGSQGRA HALNLKDSGA ANVRVALREG SATVQKAQAD 70 80 90 100 110 120 GFEVMNVADA AKWADLMMMA TPDELQADIY RDHIHNNLRD GAAIAFAHGL NVHCGLIEPK 130 140 150 160 170 180 KTVDVMMIAP KGPGHTVRGE YQKGGGVPCL IAIHQDASGN AHDLALSYAS GVGGGRSGVI 190 200 210 220 230 240 ETTFKEECET DLFGEQAVLC GGVVELIRTG FEVLVEAGYA PEMAYFECLN EMKLIVDLIY 250 260 270 280 290 300 EGGIANMNYS ISNTAEWGEY VTGPRIITAE TKAEMKRVLK DIQTGKFTSD WMQEWKAGAA 310 320 330 RFKGIRRLND AHQIEEVGGK LRAMMPWIEK NKLVDKARN

References

[1]

"Brucella melitensis ATCC 23457 whole genome shotgun sequencing project." Setubal J.C., Boyle S., Crasta O.R., Gillespie J.J., Kenyon R.W., Lu J., Mane S., Nagrani S., Shallom J.M., Shallom S., Shukla M., Snyder E.E., Sobral B.W., Wattam A.R., Will R., Williams K., Yoo H., Munk C. , Tapia R., Han C., Detter J.C., Bruce D., Brettin T.S. Submitted (MAR-2009) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 23457.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001488 Genomic DNA. Translation: ACO01142.1.
RefSeq	YP_002733096.1. NC_012441.1.
3D structure databases
ProteinModelPortal	C0RE20.
SMR	C0RE20. Positions 1-328.
ModBase	Search...
Protein-protein interaction databases
STRING	C0RE20.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	7676492.
GenomeReviews	Gene locus BMEA_A1427 in contig CP001488_GR.
KEGG	bmi:BMEA_A1427.
PATRIC	17838626. VBIBruMel14466_1450.
Organism-specific databases
CMR	Search...
Phylogenomic databases
OMA	MAYAKGI.
ProtClustDB	PRK05479.
Family and domain databases
HAMAP	MF_00435. IlvC. [Tree]
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR013023. AcH_isomrdctse. IPR000506. AcH_isomrdctse_C. IPR013328. DH_multihelical. IPR013116. IlvN. [Graphical view]
Gene3D	G3DSA:1.10.1040.10. Opine_DH. 1 hit.
KO	K00053.
PANTHER	PTHR21371. AcH_isomrdctse. 1 hit.
Pfam	PF01450. IlvC. 1 hit. PF07991. IlvN. 1 hit. [Graphical view]
SUPFAM	SSF48179. 6DGDH_C_like. 1 hit.
TIGRFAMs	TIGR00465. IlvC. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ILVC_BRUMB

Accession

Primary (citable) accession number: C0RE20

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 5, 2009
Last modified:	January 25, 2012
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program