C0R5T5 (C0R5T5_WOLWR) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase 1 HAMAP MF_00022
EC=6.1.1.17 HAMAP MF_00022

Alternative name(s):
Glutamyl-tRNA synthetase 1 HAMAP MF_00022

Gene names

Name:	gltX1 HAMAP MF_00022
Ordered Locus Names:	WRi_003090

Organism

Wolbachia sp. subsp. Drosophila simulans (strain wRi) [Complete proteome] [HAMAP]

Taxonomic identifier

66084 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rickettsiales › Anaplasmataceae › Wolbachieae › Wolbachia

Protein attributes

Sequence length	476 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022
Subunit structure	Monomer By similarity. HAMAP MF_00022
Subcellular location	Cytoplasm By similarity HAMAP MF_00022.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. HAMAP MF_00022 RuleBase RU003489

Ontologies

Keywords
Biological process	Protein biosynthesis HAMAP MF_00022 RuleBase RU003489
Cellular component	Cytoplasm HAMAP MF_00022
Ligand	ATP-binding HAMAP MF_00022 RuleBase RU003489 Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase HAMAP MF_00022 RuleBase RU003489 EMBL ACN95127.1 Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP glutamate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Regions
	Motif	10 – 20	11	"HIGH" region By similarity HAMAP MF_00022
	Motif	255 – 259	5	"KMSKS" region By similarity HAMAP MF_00022
Sites
	Binding site	258	1	ATP By similarity HAMAP MF_00022

Sequences

	Sequence		Length	Mass (Da)
	C0R5T5 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: B508474EB24951B0	FASTA	476	54,408
	10 20 30 40 50 60 MLDVVTRFAP SPTGFLHIGG ARTALFNWLY AKHHGGRFLL RIEDTDRKRS TKEAIDAIIE 70 80 90 100 110 120 GLRWLGVSYD GEIVYQSKRI ERHKEVANLL VEKGRAYHCY CPEDEVAEKK IKAREEGKIY 130 140 150 160 170 180 KHKCTTKPPS CHPSSRHWDP EENMWSRAGM TSGVRSVIRF KVPDSQEIVI DDKIYGQIKV 190 200 210 220 230 240 NSNQLDDIVI LRSDNTPTYI FAVVIDDHDA GITDIIRGSD HLTNTFKHLL IYQALDFDIP 250 260 270 280 290 300 RFAHVPLIHG EDGNKLSKRH GATSVCDYEK MGILPKAMRN YLLRLGWSHG NDEIISDEQA 310 320 330 340 350 360 IEWFNLESIG RSPARLDFKK LEHLNNHYIS NMSNEDILTL MLRENTLTDK KKGYLLQGLT 370 380 390 400 410 420 ELKKRANYLT ELLDLAKFYI QDPPLDLSEE AEQIVKSNLD IIKLLASFLS EIGDENWNKG 430 440 450 460 470 FLSSQIKECA KLHDMKISDV YHSLRAPITG VMDAPGIIDI MVILGKDECI RRLQAI

References

[1]

"The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans." Klasson L., Westberg J., Sapountzis P., Naeslund K., Lutnaes Y., Darby A.C., Veneti Z., Chen L., Braig H.R., Garrett R., Bourtzis K., Andersson S.G. Proc. Natl. Acad. Sci. U.S.A. 106:5725-5730(2009) [PubMed: 19307581] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001391 Genomic DNA. Translation: ACN95127.1.
RefSeq	YP_002726918.1. NC_012416.1.
3D structure databases
ProteinModelPortal	C0R5T5.
ModBase	Search...
Protein-protein interaction databases
STRING	C0R5T5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBWOLT00000002251; EBWOLP00000002101; EBWOLG00000002251.
GeneID	7669050.
GenomeReviews	Gene locus WRi_003090 in contig CP001391_GR.
KEGG	wri:WRi_003090.
PATRIC	24034687. VBIWolSp98304_0358.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000030976.
OMA	ANFNDES.
ProtClustDB	PRK01406.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C0R5T5_WOLWR

Accession

Primary (citable) accession number: C0R5T5

Entry history

Integrated into UniProtKB/TrEMBL:	May 5, 2009
Last sequence update:	May 5, 2009
Last modified:	January 25, 2012
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)