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C0R573 (SYL_WOLWR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 41. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:WRi_000480
OrganismWolbachia sp. subsp. Drosophila simulans (strain wRi) [Complete proteome] [HAMAP]
Taxonomic identifier66084 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length838 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 838838Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199234

Regions

Motif36 – 4611"HIGH" region HAMAP-Rule MF_00049
Motif611 – 6155"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6141ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C0R573 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 59AC758234BF408D

FASTA83895,498
        10         20         30         40         50         60 
MKYDFKNVER FCQDKWDFSV SKSSKQEKCY VLEMFPYPSG KIHMGHLRNY AIGDVIARYK 

        70         80         90        100        110        120 
RARGFEVLHP IGWDAFGLPA ENAARDNNIS PEIWTKENID NMRAQLKSIG LSYNWNRELS 

       130        140        150        160        170        180 
TCEPNYYTHE QKFFLDFLKH GLAYRKESWV NWDPVDQTVL ANEQVVDGKG WRSGAVVEKR 

       190        200        210        220        230        240 
KLSQWFLKIT DFAEDLLKCL QSLKNWPEKV KTMQERWIGK SEGATIEFEV VGLNKKLKVF 

       250        260        270        280        290        300 
TTYPHTLFGA SFCAVAAEHP IVQDLKNGSS VVIPVLDTGI QEIKSKREND EKIGVYTGLN 

       310        320        330        340        350        360 
VKHPFLDKEL PLYIANFVLM EYGEGAIFGC PAHDQRDFEF AQKYNLPIIP VISSAHLGVI 

       370        380        390        400        410        420 
PARDQNSYNG SQCQATQMTK EAYTGDGVMF NSEFLNGLMV SEAKEVIIKK LKEKGIGKKT 

       430        440        450        460        470        480 
TNYRLHDWGV SRQRYWGCPI PIIYCKDCGT VPVPEKDLPV ILPADVEFTS GGNPLDKHPT 

       490        500        510        520        530        540 
WKFVDCPKCG KQAERETDTF DTFFESSWYF AAFCSEDKSI DKDACNRFMP VDYYIGGIEH 

       550        560        570        580        590        600 
AILHLLYSRF FCRALTKCGY FDIKEPFSTL ITQGMVCHAT YKDENGKWLF LAEAKELIAR 

       610        620        630        640        650        660 
GTKVQVGKVE KMSKSKKNTV DPNFIIEKYG ADTARLFVLS DTPPEKDMEW SDDGVEGCSR 

       670        680        690        700        710        720 
YVNKLWRMVM QLKPVNMHYD NKSVTGGLLE YRKKIHKLLH GLTDDLENCR LNCVVAKFRE 

       730        740        750        760        770        780 
MTNLIAEIDV AAGKSLIDEG ICILIRVIEP FMPHLAESLW QEIGGQPWPK ADESLLVDDT 

       790        800        810        820        830 
VTIAVQINGK LRATIEVAIN LPQEELKKIA IDSVSSKIDQ NKVRTVYAVP NKIVNIVI 

« Hide

References

[1]"The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans."
Klasson L., Westberg J., Sapountzis P., Naeslund K., Lutnaes Y., Darby A.C., Veneti Z., Chen L., Braig H.R., Garrett R., Bourtzis K., Andersson S.G.
Proc. Natl. Acad. Sci. U.S.A. 106:5725-5730(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: wRi.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001391 Genomic DNA. Translation: ACN94915.1.
RefSeqYP_002726706.1. NC_012416.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING66084.WRi_000480.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACN94915; ACN94915; WRi_000480.
GeneID7668806.
KEGGwri:WRi_000480.
PATRIC24034060. VBIWolSp98304_0049.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycWSP66084:GHAN-48-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_WOLWR
AccessionPrimary (citable) accession number: C0R573
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 41 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries