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C0R4N2 (DAPF_WOLWR) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Diaminopimelate epimerase

Short name=DAP epimerase
EC=5.1.1.7
Gene names
Name:dapF
Ordered Locus Names:WRi_011860
OrganismWolbachia sp. subsp. Drosophila simulans (strain wRi) [Complete proteome] [HAMAP]
Taxonomic identifier66084 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia

Protein attributes

Sequence length262 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan By similarity. HAMAP-Rule MF_00197

Catalytic activity

LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate. HAMAP-Rule MF_00197

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; DL-2,6-diaminopimelate from LL-2,6-diaminopimelate: step 1/1. HAMAP-Rule MF_00197

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00197.

Sequence similarities

Belongs to the diaminopimelate epimerase family.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Lysine biosynthesis
   Cellular componentCytoplasm
   Molecular functionIsomerase
   PTMDisulfide bond
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processlysine biosynthetic process via diaminopimelate

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functiondiaminopimelate epimerase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 262262Diaminopimelate epimerase HAMAP-Rule MF_00197
PRO_1000124434

Regions

Region72 – 743Substrate binding By similarity
Region205 – 2062Substrate binding By similarity

Sites

Active site721Proton donor/acceptor By similarity
Active site2141Proton donor/acceptor By similarity
Binding site171Substrate By similarity
Binding site451Substrate By similarity
Binding site631Substrate By similarity
Binding site1541Substrate By similarity
Binding site1871Substrate By similarity
Site1561Important for catalytic activity By similarity
Site2051Important for catalytic activity By similarity

Amino acid modifications

Disulfide bond72 ↔ 214 HAMAP-Rule MF_00197

Sequences

Sequence LengthMass (Da)Tools
C0R4N2 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: AAF95D34BE4AAEDE

FASTA26228,665
        10         20         30         40         50         60 
MSGQNSENFI KMHGTGNNFV IIDSCSANDL NWNYKKIANQ NGCDQVIVIT NSSLADCFMH 

        70         80         90        100        110        120 
IYNADGSKAE MCGNAARCVG YLLMSEKGTE YITIELVNKR ILECFKVGDK SIKVNMGKPL 

       130        140        150        160        170        180 
LKWHKIPLST ECDTLHLPIE LEMLKDPVAV NIGNPHIVFF VDSVNEIPLQ SLGPKLENHA 

       190        200        210        220        230        240 
LFPQKINISI AQVEKSGEIA LRVWERGTGI TASCGSAACA ALVTSTLRGY LTVQQTSVNL 

       250        260 
PGGKLLIEWA NNVFMTGDIG FL 

« Hide

References

[1]"The mosaic genome structure of the Wolbachia wRi strain infecting Drosophila simulans."
Klasson L., Westberg J., Sapountzis P., Naeslund K., Lutnaes Y., Darby A.C., Veneti Z., Chen L., Braig H.R., Garrett R., Bourtzis K., Andersson S.G.
Proc. Natl. Acad. Sci. U.S.A. 106:5725-5730(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: wRi.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001391 Genomic DNA. Translation: ACN95874.1.
RefSeqYP_002727665.1. NC_012416.1.

3D structure databases

ProteinModelPortalC0R4N2.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING66084.WRi_011860.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACN95874; ACN95874; WRi_011860.
GeneID7669898.
KEGGwri:WRi_011860.
PATRIC24036716. VBIWolSp98304_1349.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0253.
HOGENOMHOG000220466.
KOK01778.
OMAKMRIFNN.
OrthoDBEOG6ND0M5.

Enzyme and pathway databases

BioCycWSP66084:GHAN-1118-MONOMER.
UniPathwayUPA00034; UER00025.

Family and domain databases

HAMAPMF_00197. DAP_epimerase.
InterProIPR018510. DAP_epimerase_AS.
IPR001653. DAP_epimerase_DapF.
[Graphical view]
PANTHERPTHR31689. PTHR31689. 1 hit.
PfamPF01678. DAP_epimerase. 2 hits.
[Graphical view]
TIGRFAMsTIGR00652. DapF. 1 hit.
PROSITEPS01326. DAP_EPIMERASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPF_WOLWR
AccessionPrimary (citable) accession number: C0R4N2
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways