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Protein

Phosphatidylserine decarboxylase proenzyme

Gene

psd

Organism
Wolbachia sp. subsp. Drosophila simulans (strain wRi)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer).UniRule annotation

Catalytic activityi

Phosphatidyl-L-serine = phosphatidylethanolamine + CO2.UniRule annotation

Cofactori

pyruvateUniRule annotationNote: Binds 1 pyruvoyl group covalently per subunit.UniRule annotation

Pathwayi: phosphatidylethanolamine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylethanolamine from CDP-diacylglycerol.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphatidylserine decarboxylase proenzyme (psd)
This subpathway is part of the pathway phosphatidylethanolamine biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylethanolamine from CDP-diacylglycerol, the pathway phosphatidylethanolamine biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei186Schiff-base intermediate with substrate; via pyruvic acidUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Decarboxylase, Lyase

Keywords - Biological processi

Lipid biosynthesis, Lipid metabolism, Phospholipid biosynthesis, Phospholipid metabolism

Keywords - Ligandi

Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00558; UER00616.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylserine decarboxylase proenzymeUniRule annotation (EC:4.1.1.65UniRule annotation)
Cleaved into the following 2 chains:
Phosphatidylserine decarboxylase alpha chainUniRule annotation
Phosphatidylserine decarboxylase beta chainUniRule annotation
Gene namesi
Name:psdUniRule annotation
Ordered Locus Names:WRi_010820
OrganismiWolbachia sp. subsp. Drosophila simulans (strain wRi)
Taxonomic identifieri66084 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
Proteomesi
  • UP000001293 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Peripheral membrane protein UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001476471 – 185Phosphatidylserine decarboxylase beta chainUniRule annotationAdd BLAST185
ChainiPRO_1000147648186 – 230Phosphatidylserine decarboxylase alpha chainUniRule annotationAdd BLAST45

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei186Pyruvic acid (Ser); by autocatalysisUniRule annotation1

Post-translational modificationi

Is synthesized initially as an inactive proenzyme. Formation of the active enzyme involves a self-maturation process in which the active site pyruvoyl group is generated from an internal serine residue via an autocatalytic post-translational modification. Two non-identical subunits are generated from the proenzyme in this reaction, and the pyruvate is formed at the N-terminus of the alpha chain, which is derived from the carboxyl end of the proenzyme. The post-translation cleavage follows an unusual pathway, termed non-hydrolytic serinolysis, in which the side chain hydroxyl group of the serine supplies its oxygen atom to form the C-terminus of the beta chain, while the remainder of the serine residue undergoes an oxidative deamination to produce ammonia and the pyruvoyl prosthetic group on the alpha chain.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei185 – 186Cleavage (non-hydrolytic); by autocatalysisUniRule annotation2

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Heterodimer of a large membrane-associated beta subunit and a small pyruvoyl-containing alpha subunit.UniRule annotation

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphatidylserine decarboxylase family. PSD-A subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000229359.
KOiK01613.
OMAiFNVHSNR.

Family and domain databases

HAMAPiMF_00664. PS_decarb_PSD_A. 1 hit.
InterProiIPR003817. PS_Dcarbxylase.
IPR033175. PSD-A.
[Graphical view]
PfamiPF02666. PS_Dcarbxylase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00164. PS_decarb_rel. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0R4E0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCFGLPNINR EGYSFIVVSF IVTCIAFSIS WGFGVTCLFP TLLCTYFFRD
60 70 80 90 100
PARAVPNNKN LILSPADGVI SKIEEVNYPL SAENGEEKKF TLVSIFLSVL
110 120 130 140 150
NVHVNRIPIS GTIKEMSYKK GKFVSAMSNR SSNENEKQVI VIEYEKGKEI
160 170 180 190 200
IVEQIAGLIA RRIVCNLGIS QNVKAGERFG IIRFGSRVNI YVPADTEVRV
210 220 230
SEGQTVIGGE TIIANLNKEN VQEKLTFDVI
Length:230
Mass (Da):25,537
Last modified:May 5, 2009 - v1
Checksum:iBDA9E9E86159BA02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001391 Genomic DNA. Translation: ACN95782.1.
RefSeqiWP_007548750.1. NC_012416.1.

Genome annotation databases

EnsemblBacteriaiACN95782; ACN95782; WRi_010820.
KEGGiwri:WRi_010820.
PATRICi24036486. VBIWolSp98304_1236.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001391 Genomic DNA. Translation: ACN95782.1.
RefSeqiWP_007548750.1. NC_012416.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACN95782; ACN95782; WRi_010820.
KEGGiwri:WRi_010820.
PATRICi24036486. VBIWolSp98304_1236.

Phylogenomic databases

HOGENOMiHOG000229359.
KOiK01613.
OMAiFNVHSNR.

Enzyme and pathway databases

UniPathwayiUPA00558; UER00616.

Family and domain databases

HAMAPiMF_00664. PS_decarb_PSD_A. 1 hit.
InterProiIPR003817. PS_Dcarbxylase.
IPR033175. PSD-A.
[Graphical view]
PfamiPF02666. PS_Dcarbxylase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00164. PS_decarb_rel. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPSD_WOLWR
AccessioniPrimary (citable) accession number: C0R4E0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: November 2, 2016
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.