SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C0R2Q4

- LIPA_WOLWR

UniProt

C0R2Q4 - LIPA_WOLWR

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Lipoyl synthase
Gene
lipA, WRi_003970
Organism
Wolbachia sp. subsp. Drosophila simulans (strain wRi)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi34 – 341Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi39 – 391Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi45 – 451Iron-sulfur 1 (4Fe-4S) By similarity
Metal bindingi60 – 601Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi64 – 641Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi67 – 671Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
  2. lipoate synthase activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. protein lipoylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciWSP66084:GHAN-366-MONOMER.
UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase (EC:2.8.1.8)
Alternative name(s):
Lip-syn
Short name:
LS
Lipoate synthase
Lipoic acid synthase
Sulfur insertion protein LipA
Gene namesi
Name:lipA
Ordered Locus Names:WRi_003970
OrganismiWolbachia sp. subsp. Drosophila simulans (strain wRi)
Taxonomic identifieri66084 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRickettsialesAnaplasmataceaeWolbachieaeWolbachia
ProteomesiUP000001293: Chromosome

Subcellular locationi

Cytoplasm Reviewed prediction UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 287287Lipoyl synthaseUniRule annotation
PRO_1000124650Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi66084.WRi_003970.

Structurei

3D structure databases

ProteinModelPortaliC0R2Q4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0320.
HOGENOMiHOG000235998.
KOiK03644.
OMAiHPHIPTK.
OrthoDBiEOG6038ZS.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

C0R2Q4-1 [UniParc]FASTAAdd to Basket

« Hide

MHSKPQWLRA KAPTGEVFNE TLNIVKLHNL HTVCEEAACP NIGECWNKRH    50
ATVMILGSVC TRACAFCNVA TGIPDKLDPH EPENLAKAIK KLNLKHVVIT 100
SVDRDDLPDG GANQFIQCIE EIRKITSETT IEILTPDFLN KKGAFEAIAV 150
ASPDVYNHNI ETVPRLYAKI RPRARYFHSL YLLKMVKQIN PKVFTKSGLM 200
VGLGETKEEI FQVMDDLRSA EVDFITIGQY LQPTPKHAKL DRYVTPEEFE 250
HYKYIAYSKG FLVVASSPLT RSSYHAEEDF NRLKACR 287
Length:287
Mass (Da):32,531
Last modified:May 5, 2009 - v1
Checksum:i307A180FBE6C0A6D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001391 Genomic DNA. Translation: ACN95196.1.
RefSeqiWP_012673150.1. NC_012416.1.
YP_002726987.1. NC_012416.1.

Genome annotation databases

EnsemblBacteriaiACN95196; ACN95196; WRi_003970.
GeneIDi7669132.
KEGGiwri:WRi_003970.
PATRICi24034885. VBIWolSp98304_0454.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001391 Genomic DNA. Translation: ACN95196.1 .
RefSeqi WP_012673150.1. NC_012416.1.
YP_002726987.1. NC_012416.1.

3D structure databases

ProteinModelPortali C0R2Q4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 66084.WRi_003970.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACN95196 ; ACN95196 ; WRi_003970 .
GeneIDi 7669132.
KEGGi wri:WRi_003970.
PATRICi 24034885. VBIWolSp98304_0454.

Phylogenomic databases

eggNOGi COG0320.
HOGENOMi HOG000235998.
KOi K03644.
OMAi HPHIPTK.
OrthoDBi EOG6038ZS.

Enzyme and pathway databases

UniPathwayi UPA00538 ; UER00593 .
BioCyci WSP66084:GHAN-366-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_00206. Lipoyl_synth.
InterProi IPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view ]
PANTHERi PTHR10949. PTHR10949. 1 hit.
Pfami PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
SMARTi SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00510. lipA. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: wRi.

Entry informationi

Entry nameiLIPA_WOLWR
AccessioniPrimary (citable) accession number: C0R2Q4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: September 3, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi