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C0R1H5 (ASSY_BRAHW) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 37. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Argininosuccinate synthase

EC=6.3.4.5
Alternative name(s):
Citrulline--aspartate ligase
Gene names
Name:argG
Ordered Locus Names:BHWA1_01491
OrganismBrachyspira hyodysenteriae (strain ATCC 49526 / WA1) [Complete proteome] [HAMAP]
Taxonomic identifier565034 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira

Protein attributes

Sequence length406 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-citrulline + L-aspartate = AMP + diphosphate + N(omega)-(L-arginino)succinate. HAMAP-Rule MF_00005

Pathway

Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 2/3. HAMAP-Rule MF_00005

Subunit structure

Homotetramer By similarity. HAMAP-Rule MF_00005

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00005.

Sequence similarities

Belongs to the argininosuccinate synthase family. Type 1 subfamily.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Arginine biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processarginine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

argininosuccinate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 406406Argininosuccinate synthase HAMAP-Rule MF_00005
PRO_1000191882

Regions

Nucleotide binding8 – 169ATP By similarity

Sites

Binding site861Citrulline By similarity
Binding site911Citrulline By similarity
Binding site1161ATP; via amide nitrogen By similarity
Binding site1181Aspartate By similarity
Binding site1221Aspartate By similarity
Binding site1221Citrulline By similarity
Binding site1231Aspartate By similarity
Binding site1261Citrulline By similarity
Binding site1751Citrulline By similarity
Binding site1841Citrulline By similarity
Binding site2611Citrulline By similarity
Binding site2731Citrulline By similarity

Sequences

Sequence LengthMass (Da)Tools
C0R1H5 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: F85996AB841BFAE9

FASTA40645,587
        10         20         30         40         50         60 
MKKKLVLAYS GGLDTTVIIP WLKENYDYDV IAVCVDVGQG TETDGLEEKA LKTGAVKYRL 

        70         80         90        100        110        120 
VKCEDEFVTD YIYPIVKAEA TYEDKYLLGT SAARPLIAKK LVEVALEEGA TAIAHGATGK 

       130        140        150        160        170        180 
GNDQVRFELT VKALAPNFEI IAPWREWNIS SREEEIKYLE DRNIEVPMKK DDSYSRDKNL 

       190        200        210        220        230        240 
WHLSHEGLEL EDPANMPNYE RLLKLSNTIE NAPNEGQFVE LEFEKGIPTK VDGKTFSPSD 

       250        260        270        280        290        300 
LVKYLNEIGG KHAVGIVDLL ENRVVGIKCR GVYETPGGTI LYAAHREIEH LCLDRETLYF 

       310        320        330        340        350        360 
KHVVSHKLTD LVYSGRWFTP LREALCAFID STQQTVTGKV KLKLYKGNII PAGVTSPYSL 

       370        380        390        400 
YNQSLASFTT GELYDHHDAQ GFITLFGLPL KVNALMKEQA KKMGLK 

« Hide

References

[1]"Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49526 / WA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001357 Genomic DNA. Translation: ACN83963.1.
RefSeqYP_002721667.1. NC_012225.1.

3D structure databases

ProteinModelPortalC0R1H5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING565034.BHWA1_01491.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACN83963; ACN83963; BHWA1_01491.
GeneID7666587.
KEGGbhy:BHWA1_01491.
PATRIC21181051. VBIBraHyo62857_1455.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0137.
HOGENOMHOG000230093.
KOK01940.
OMAIYNGYWW.
OrthoDBEOG6K9QCV.
ProtClustDBCLSK2810133.

Enzyme and pathway databases

BioCycBHYO565034:GJI7-1483-MONOMER.
UniPathwayUPA00068; UER00113.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
3.90.1260.10. 1 hit.
HAMAPMF_00005. Arg_succ_synth_type1.
InterProIPR001518. Arginosuc_synth.
IPR018223. Arginosuc_synth_CS.
IPR023434. Arginosuc_synth_type_1_subfam.
IPR024074. AS_cat/multimer_dom_body.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamPF00764. Arginosuc_synth. 1 hit.
[Graphical view]
TIGRFAMsTIGR00032. argG. 1 hit.
PROSITEPS00564. ARGININOSUCCIN_SYN_1. 1 hit.
PS00565. ARGININOSUCCIN_SYN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameASSY_BRAHW
AccessionPrimary (citable) accession number: C0R1H5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: February 19, 2014
This is version 37 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways