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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-1315-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BHWA1_01321
OrganismiBrachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Taxonomic identifieri565034 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesBrachyspiralesBrachyspiraceaeBrachyspira
ProteomesiUP000001803 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Isoleucine--tRNA ligasePRO_1000189131Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi565034.BHWA1_01321.

Structurei

3D structure databases

ProteinModelPortaliC0R112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0R112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSSTINLP KTAFPMKAGL KEKEPKIIKK WEEEKLYQQL RELRKGAPKC
60 70 80 90 100
ILHDGPPYAN GDIHIGTSLN KIIKDIIVRY KSAKGFDSPY VPGWDCHGMP
110 120 130 140 150
IELKVQESLG DKYKETSKFI MRKKCRAYAQ KYIDIQRKEF KRLGVMGDWE
160 170 180 190 200
NPYLTMSPEY ESEIVEVFAQ LVEKGYIYKG LRTIHWCMDC ETALAAAEIE
210 220 230 240 250
YDDNHTSTSV YVRFPVLNKI NDKLDGNVDV MIWTTTPWTL PSNMACAFNR
260 270 280 290 300
DLEYVAVEID GRYAIMTTSL VDTVLSKKDM KAEGRDMIPV SMEDIEKLEI
310 320 330 340 350
AHPFIKDRKS AVVFADYVEA TAGTGIVHTA PGHGMEDYQT GMNYGLEIYC
360 370 380 390 400
PVDKAGRYTS DFPEMQGMKV RDANPKVVEI LENNGSLYHK EKVTHSYPIC
410 420 430 440 450
WRCKNPLIFR ATSQWFMNMT HDDIDKRTVK ALDNIKWYPT WGHDRMQKML
460 470 480 490 500
ENRPDWCLSR QRSWGVPIPA FYCKNCGKTL LTAESTRHFA EIVKTKGMDV
510 520 530 540 550
WFELEAKDLL PEGTKCECGS TDFDKEQDIL DVWFDSGVSS FAAQKTNKDL
560 570 580 590 600
DGVFPVDIYL EGGDQYRGWF QAAIWPSMAI RGIPPYKELV THGWTLDEQG
610 620 630 640 650
RAMHKSAGNV VSPLEVIDKY GADILRLWCI SEDFTHNARV GDNMMKAIAD
660 670 680 690 700
NYRKIRNTFR YLLGNISDFD FTKEKIEVKD LLPVDRYALS RLHSFIKVAE
710 720 730 740 750
KACDGYEFHL FYQRLINYCV VELSATYFDI IKDRLYCDRK DSVSRRSAQT
760 770 780 790 800
VLVEILDVLV KLIAPVLPFT TDEVWGYYKG ENASSVHLEL YPKADDNLID
810 820 830 840 850
LELEKEWTSI LKVRDDVLLS LERARDNSTI GKSLEAYVTI CTKEPATKEL
860 870 880 890 900
LTKYEKYLNE IFIVSKVTLS DSKDDTFIEG GVSFVKTEKA SHEKCVRCWG
910 920
HYDSVGTDSE HKELCTRCAE AVR
Length:923
Mass (Da):106,036
Last modified:May 5, 2009 - v1
Checksum:i8CD96E23E8FC1439
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN83800.1.
RefSeqiWP_012670845.1. NC_012225.1.

Genome annotation databases

EnsemblBacteriaiACN83800; ACN83800; BHWA1_01321.
GeneIDi24366053.
KEGGibhy:BHWA1_01321.
PATRICi21180717. VBIBraHyo62857_1293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN83800.1.
RefSeqiWP_012670845.1. NC_012225.1.

3D structure databases

ProteinModelPortaliC0R112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi565034.BHWA1_01321.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACN83800; ACN83800; BHWA1_01321.
GeneIDi24366053.
KEGGibhy:BHWA1_01321.
PATRICi21180717. VBIBraHyo62857_1293.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-1315-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
    Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
    PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49526 / WA1.

Entry informationi

Entry nameiSYI_BRAHW
AccessioniPrimary (citable) accession number: C0R112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: July 22, 2015
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.