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C0R112

- SYI_BRAHW

UniProt

C0R112 - SYI_BRAHW

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (05 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
    Binding sitei605 – 6051ATPUniRule annotation
    Metal bindingi895 – 8951ZincUniRule annotation
    Metal bindingi898 – 8981ZincUniRule annotation
    Metal bindingi915 – 9151ZincUniRule annotation
    Metal bindingi918 – 9181ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciBHYO565034:GJI7-1315-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:BHWA1_01321
    OrganismiBrachyspira hyodysenteriae (strain ATCC 49526 / WA1)
    Taxonomic identifieri565034 [NCBI]
    Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira
    ProteomesiUP000001803: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 923923Isoleucine--tRNA ligasePRO_1000189131Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi565034.BHWA1_01321.

    Structurei

    3D structure databases

    ProteinModelPortaliC0R112.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi602 – 6065"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiYETLYEC.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C0R112-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYSSTINLP KTAFPMKAGL KEKEPKIIKK WEEEKLYQQL RELRKGAPKC    50
    ILHDGPPYAN GDIHIGTSLN KIIKDIIVRY KSAKGFDSPY VPGWDCHGMP 100
    IELKVQESLG DKYKETSKFI MRKKCRAYAQ KYIDIQRKEF KRLGVMGDWE 150
    NPYLTMSPEY ESEIVEVFAQ LVEKGYIYKG LRTIHWCMDC ETALAAAEIE 200
    YDDNHTSTSV YVRFPVLNKI NDKLDGNVDV MIWTTTPWTL PSNMACAFNR 250
    DLEYVAVEID GRYAIMTTSL VDTVLSKKDM KAEGRDMIPV SMEDIEKLEI 300
    AHPFIKDRKS AVVFADYVEA TAGTGIVHTA PGHGMEDYQT GMNYGLEIYC 350
    PVDKAGRYTS DFPEMQGMKV RDANPKVVEI LENNGSLYHK EKVTHSYPIC 400
    WRCKNPLIFR ATSQWFMNMT HDDIDKRTVK ALDNIKWYPT WGHDRMQKML 450
    ENRPDWCLSR QRSWGVPIPA FYCKNCGKTL LTAESTRHFA EIVKTKGMDV 500
    WFELEAKDLL PEGTKCECGS TDFDKEQDIL DVWFDSGVSS FAAQKTNKDL 550
    DGVFPVDIYL EGGDQYRGWF QAAIWPSMAI RGIPPYKELV THGWTLDEQG 600
    RAMHKSAGNV VSPLEVIDKY GADILRLWCI SEDFTHNARV GDNMMKAIAD 650
    NYRKIRNTFR YLLGNISDFD FTKEKIEVKD LLPVDRYALS RLHSFIKVAE 700
    KACDGYEFHL FYQRLINYCV VELSATYFDI IKDRLYCDRK DSVSRRSAQT 750
    VLVEILDVLV KLIAPVLPFT TDEVWGYYKG ENASSVHLEL YPKADDNLID 800
    LELEKEWTSI LKVRDDVLLS LERARDNSTI GKSLEAYVTI CTKEPATKEL 850
    LTKYEKYLNE IFIVSKVTLS DSKDDTFIEG GVSFVKTEKA SHEKCVRCWG 900
    HYDSVGTDSE HKELCTRCAE AVR 923
    Length:923
    Mass (Da):106,036
    Last modified:May 5, 2009 - v1
    Checksum:i8CD96E23E8FC1439
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001357 Genomic DNA. Translation: ACN83800.1.
    RefSeqiWP_012670845.1. NC_012225.1.
    YP_002721504.1. NC_012225.1.

    Genome annotation databases

    EnsemblBacteriaiACN83800; ACN83800; BHWA1_01321.
    GeneIDi7666421.
    KEGGibhy:BHWA1_01321.
    PATRICi21180717. VBIBraHyo62857_1293.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001357 Genomic DNA. Translation: ACN83800.1 .
    RefSeqi WP_012670845.1. NC_012225.1.
    YP_002721504.1. NC_012225.1.

    3D structure databases

    ProteinModelPortali C0R112.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 565034.BHWA1_01321.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACN83800 ; ACN83800 ; BHWA1_01321 .
    GeneIDi 7666421.
    KEGGi bhy:BHWA1_01321.
    PATRICi 21180717. VBIBraHyo62857_1293.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi YETLYEC.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci BHYO565034:GJI7-1315-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
      Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
      PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 49526 / WA1.

    Entry informationi

    Entry nameiSYI_BRAHW
    AccessioniPrimary (citable) accession number: C0R112
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3