Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei561Aminoacyl-adenylateUniRule annotation1
Binding sitei605ATPUniRule annotation1
Metal bindingi895ZincUniRule annotation1
Metal bindingi898ZincUniRule annotation1
Metal bindingi915ZincUniRule annotation1
Metal bindingi918ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHYO565034:G1GUM-1288-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BHWA1_01321
OrganismiBrachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Taxonomic identifieri565034 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesBrachyspiralesBrachyspiraceaeBrachyspira
Proteomesi
  • UP000001803 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10001891311 – 923Isoleucine--tRNA ligaseAdd BLAST923

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi565034.BHWA1_01321

Structurei

3D structure databases

ProteinModelPortaliC0R112
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi602 – 606"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

C0R112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYSSTINLP KTAFPMKAGL KEKEPKIIKK WEEEKLYQQL RELRKGAPKC
60 70 80 90 100
ILHDGPPYAN GDIHIGTSLN KIIKDIIVRY KSAKGFDSPY VPGWDCHGMP
110 120 130 140 150
IELKVQESLG DKYKETSKFI MRKKCRAYAQ KYIDIQRKEF KRLGVMGDWE
160 170 180 190 200
NPYLTMSPEY ESEIVEVFAQ LVEKGYIYKG LRTIHWCMDC ETALAAAEIE
210 220 230 240 250
YDDNHTSTSV YVRFPVLNKI NDKLDGNVDV MIWTTTPWTL PSNMACAFNR
260 270 280 290 300
DLEYVAVEID GRYAIMTTSL VDTVLSKKDM KAEGRDMIPV SMEDIEKLEI
310 320 330 340 350
AHPFIKDRKS AVVFADYVEA TAGTGIVHTA PGHGMEDYQT GMNYGLEIYC
360 370 380 390 400
PVDKAGRYTS DFPEMQGMKV RDANPKVVEI LENNGSLYHK EKVTHSYPIC
410 420 430 440 450
WRCKNPLIFR ATSQWFMNMT HDDIDKRTVK ALDNIKWYPT WGHDRMQKML
460 470 480 490 500
ENRPDWCLSR QRSWGVPIPA FYCKNCGKTL LTAESTRHFA EIVKTKGMDV
510 520 530 540 550
WFELEAKDLL PEGTKCECGS TDFDKEQDIL DVWFDSGVSS FAAQKTNKDL
560 570 580 590 600
DGVFPVDIYL EGGDQYRGWF QAAIWPSMAI RGIPPYKELV THGWTLDEQG
610 620 630 640 650
RAMHKSAGNV VSPLEVIDKY GADILRLWCI SEDFTHNARV GDNMMKAIAD
660 670 680 690 700
NYRKIRNTFR YLLGNISDFD FTKEKIEVKD LLPVDRYALS RLHSFIKVAE
710 720 730 740 750
KACDGYEFHL FYQRLINYCV VELSATYFDI IKDRLYCDRK DSVSRRSAQT
760 770 780 790 800
VLVEILDVLV KLIAPVLPFT TDEVWGYYKG ENASSVHLEL YPKADDNLID
810 820 830 840 850
LELEKEWTSI LKVRDDVLLS LERARDNSTI GKSLEAYVTI CTKEPATKEL
860 870 880 890 900
LTKYEKYLNE IFIVSKVTLS DSKDDTFIEG GVSFVKTEKA SHEKCVRCWG
910 920
HYDSVGTDSE HKELCTRCAE AVR
Length:923
Mass (Da):106,036
Last modified:May 5, 2009 - v1
Checksum:i8CD96E23E8FC1439
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA Translation: ACN83800.1
RefSeqiWP_012670845.1, NC_012225.1

Genome annotation databases

EnsemblBacteriaiACN83800; ACN83800; BHWA1_01321
KEGGibhy:BHWA1_01321

Similar proteinsi

Entry informationi

Entry nameiSYI_BRAHW
AccessioniPrimary (citable) accession number: C0R112
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: March 28, 2018
This is version 68 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health