Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C0R112

- SYI_BRAHW

UniProt

C0R112 - SYI_BRAHW

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei561 – 5611Aminoacyl-adenylateUniRule annotation
Binding sitei605 – 6051ATPUniRule annotation
Metal bindingi895 – 8951ZincUniRule annotation
Metal bindingi898 – 8981ZincUniRule annotation
Metal bindingi915 – 9151ZincUniRule annotation
Metal bindingi918 – 9181ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-1315-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:BHWA1_01321
OrganismiBrachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Taxonomic identifieri565034 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira
ProteomesiUP000001803: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 923923Isoleucine--tRNA ligasePRO_1000189131Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi565034.BHWA1_01321.

Structurei

3D structure databases

ProteinModelPortaliC0R112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi602 – 6065"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiYETLYEC.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0R112-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MDYSSTINLP KTAFPMKAGL KEKEPKIIKK WEEEKLYQQL RELRKGAPKC
60 70 80 90 100
ILHDGPPYAN GDIHIGTSLN KIIKDIIVRY KSAKGFDSPY VPGWDCHGMP
110 120 130 140 150
IELKVQESLG DKYKETSKFI MRKKCRAYAQ KYIDIQRKEF KRLGVMGDWE
160 170 180 190 200
NPYLTMSPEY ESEIVEVFAQ LVEKGYIYKG LRTIHWCMDC ETALAAAEIE
210 220 230 240 250
YDDNHTSTSV YVRFPVLNKI NDKLDGNVDV MIWTTTPWTL PSNMACAFNR
260 270 280 290 300
DLEYVAVEID GRYAIMTTSL VDTVLSKKDM KAEGRDMIPV SMEDIEKLEI
310 320 330 340 350
AHPFIKDRKS AVVFADYVEA TAGTGIVHTA PGHGMEDYQT GMNYGLEIYC
360 370 380 390 400
PVDKAGRYTS DFPEMQGMKV RDANPKVVEI LENNGSLYHK EKVTHSYPIC
410 420 430 440 450
WRCKNPLIFR ATSQWFMNMT HDDIDKRTVK ALDNIKWYPT WGHDRMQKML
460 470 480 490 500
ENRPDWCLSR QRSWGVPIPA FYCKNCGKTL LTAESTRHFA EIVKTKGMDV
510 520 530 540 550
WFELEAKDLL PEGTKCECGS TDFDKEQDIL DVWFDSGVSS FAAQKTNKDL
560 570 580 590 600
DGVFPVDIYL EGGDQYRGWF QAAIWPSMAI RGIPPYKELV THGWTLDEQG
610 620 630 640 650
RAMHKSAGNV VSPLEVIDKY GADILRLWCI SEDFTHNARV GDNMMKAIAD
660 670 680 690 700
NYRKIRNTFR YLLGNISDFD FTKEKIEVKD LLPVDRYALS RLHSFIKVAE
710 720 730 740 750
KACDGYEFHL FYQRLINYCV VELSATYFDI IKDRLYCDRK DSVSRRSAQT
760 770 780 790 800
VLVEILDVLV KLIAPVLPFT TDEVWGYYKG ENASSVHLEL YPKADDNLID
810 820 830 840 850
LELEKEWTSI LKVRDDVLLS LERARDNSTI GKSLEAYVTI CTKEPATKEL
860 870 880 890 900
LTKYEKYLNE IFIVSKVTLS DSKDDTFIEG GVSFVKTEKA SHEKCVRCWG
910 920
HYDSVGTDSE HKELCTRCAE AVR
Length:923
Mass (Da):106,036
Last modified:May 5, 2009 - v1
Checksum:i8CD96E23E8FC1439
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN83800.1.
RefSeqiYP_002721504.1. NC_012225.1.

Genome annotation databases

EnsemblBacteriaiACN83800; ACN83800; BHWA1_01321.
GeneIDi7666421.
KEGGibhy:BHWA1_01321.
PATRICi21180717. VBIBraHyo62857_1293.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN83800.1 .
RefSeqi YP_002721504.1. NC_012225.1.

3D structure databases

ProteinModelPortali C0R112.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 565034.BHWA1_01321.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACN83800 ; ACN83800 ; BHWA1_01321 .
GeneIDi 7666421.
KEGGi bhy:BHWA1_01321.
PATRICi 21180717. VBIBraHyo62857_1293.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi YETLYEC.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci BHYO565034:GJI7-1315-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
    Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
    PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49526 / WA1.

Entry informationi

Entry nameiSYI_BRAHW
AccessioniPrimary (citable) accession number: C0R112
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: November 26, 2014
This is version 45 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3