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Protein

Peptide deformylase

Gene

def

Organism
Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions.UniRule annotation

Catalytic activityi

Formyl-L-methionyl peptide + H2O = formate + methionyl peptide.UniRule annotation

Cofactori

Fe2+UniRule annotationNote: Binds 1 Fe2+ ion.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi96 – 961IronUniRule annotation
Metal bindingi138 – 1381IronUniRule annotation
Active sitei139 – 1391UniRule annotation
Metal bindingi142 – 1421IronUniRule annotation

GO - Molecular functioni

  1. iron ion binding Source: InterPro
  2. peptide deformylase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. translation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-845-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Peptide deformylaseUniRule annotation (EC:3.5.1.88UniRule annotation)
Short name:
PDFUniRule annotation
Alternative name(s):
Polypeptide deformylaseUniRule annotation
Gene namesi
Name:defUniRule annotation
Ordered Locus Names:BHWA1_00847
OrganismiBrachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Taxonomic identifieri565034 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira
ProteomesiUP000001803 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 187187Peptide deformylasePRO_1000200716Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi565034.BHWA1_00847.

Family & Domainsi

Sequence similaritiesi

Belongs to the polypeptide deformylase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243509.
KOiK01462.
OMAiFDTMYEE.
OrthoDBiEOG664CMF.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.

Sequencei

Sequence statusi: Complete.

C0QZQ2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRELVIYGD ERLQKVSEKI EKIDDEILTL IDDMFETMYK ERGVGLAAVQ
60 70 80 90 100
IGVLKRLVVI SVPDFDDEEK PDFKLALINP EIIWHNDETE SLEEGCLSFP
110 120 130 140 150
EIRDDVARYT QIKVKYLDRE GNEQILDAEN YIAKVLQHEI DHTNGISFID
160 170 180
RLESYQKRRL KRELKELRNN TVRGIKKVNN REMLKNS
Length:187
Mass (Da):21,993
Last modified:May 4, 2009 - v1
Checksum:i23B3C836944D16D6
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN83340.1.
RefSeqiWP_012670389.1. NC_012225.1.
YP_002721044.1. NC_012225.1.

Genome annotation databases

EnsemblBacteriaiACN83340; ACN83340; BHWA1_00847.
GeneIDi7665951.
KEGGibhy:BHWA1_00847.
PATRICi21179785. VBIBraHyo62857_0837.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN83340.1.
RefSeqiWP_012670389.1. NC_012225.1.
YP_002721044.1. NC_012225.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi565034.BHWA1_00847.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACN83340; ACN83340; BHWA1_00847.
GeneIDi7665951.
KEGGibhy:BHWA1_00847.
PATRICi21179785. VBIBraHyo62857_0837.

Phylogenomic databases

eggNOGiCOG0242.
HOGENOMiHOG000243509.
KOiK01462.
OMAiFDTMYEE.
OrthoDBiEOG664CMF.

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-845-MONOMER.

Family and domain databases

Gene3Di3.90.45.10. 1 hit.
HAMAPiMF_00163. Pep_deformylase.
InterProiIPR000181. Fmet_deformylase.
IPR023635. Peptide_deformylase.
[Graphical view]
PANTHERiPTHR10458. PTHR10458. 1 hit.
PfamiPF01327. Pep_deformylase. 1 hit.
[Graphical view]
PIRSFiPIRSF004749. Pep_def. 1 hit.
PRINTSiPR01576. PDEFORMYLASE.
SUPFAMiSSF56420. SSF56420. 1 hit.
TIGRFAMsiTIGR00079. pept_deformyl. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
    Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
    PLoS ONE 4:E4641-E4641(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49526 / WA1.

Entry informationi

Entry nameiDEF_BRAHW
AccessioniPrimary (citable) accession number: C0QZQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 27, 2009
Last sequence update: May 4, 2009
Last modified: March 31, 2015
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.