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C0QWR3 (SYL_BRAHW) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:BHWA1_02260
OrganismBrachyspira hyodysenteriae (strain ATCC 49526 / WA1) [Complete proteome] [HAMAP]
Taxonomic identifier565034 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira

Protein attributes

Sequence length853 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 853853Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000199183

Regions

Motif40 – 5011"HIGH" region HAMAP-Rule MF_00049
Motif609 – 6135"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6121ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C0QWR3 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: DB3FF4804951FD5C

FASTA85398,617
        10         20         30         40         50         60 
MEYNFTTIEK KWQKFWKDNQ SFKTVSKPTD KKYYVLEMFP YPSGKMHMGH VSNYTIADSI 

        70         80         90        100        110        120 
ARYYKLLGYD ILHPMGWDAF GMPAENAAIE HKTHPAEWTL KNIANMKDQL NLLGYSYDWD 

       130        140        150        160        170        180 
REVTTCLPDY YKWGQWFILK MYEKGLLYRK GGDVNWCDHC NTVLANEQVT PEGTCWRCDG 

       190        200        210        220        230        240 
EVTKKKLEQW YIKVTDYAEQ LDADLKLLEG YWPDNVIAMQ KNWIGRSVGA YINFNLDDGK 

       250        260        270        280        290        300 
AFPIFTTRPD TIYGVTYMAI AWNYDGLLDM CTTEQKSAVE EFIKKSAKID QKTDYEKEGV 

       310        320        330        340        350        360 
FTGRYVVNPF NGEKAPLYAA NFVLAEYGSG AVMAVPAHDQ RDFEFAKKYN IPVKVVIQNA 

       370        380        390        400        410        420 
DNSLKAENMT EAYTEDGTVV NSDILNGLSS RDAIKRAIEY ATEKGFGKEK VQYKLRDWLI 

       430        440        450        460        470        480 
SRQRYWGNPL PFVHCEKCGV VPVPESELPI TLPMDIEFTV GDNPLKKSES FVNTTCPKCG 

       490        500        510        520        530        540 
GKARRETDTM DTFTCSSWYY ARYTDAHNNQ MPFDPSAANA WLGVDQYIGG IEHACMHLLY 

       550        560        570        580        590        600 
SRFWYKFMRD IGLVKGDEPF NRLLTQGMVL ANSYESRELK KFYTQEQMNN KEYEKDGIKK 

       610        620        630        640        650        660 
EDIIVKMEKM SKSKANGIDP AEIIELFGAD AVRIFVMFVA PPEKDKEWSD EGVKGSSRFL 

       670        680        690        700        710        720 
NRIWNLFLKY KDEEAFKNGK SFDYNNLSKE GQKLFRKYNK TIKKVTIDIK DRFHFNTAIA 

       730        740        750        760        770        780 
ALMELLNDMS VIKLANNDDY AMFKEVIRGY LILLNPIAPH MTEELYQILN FGKMILEERW 

       790        800        810        820        830        840 
VEHDEQYCKD DTFELVFQVN GKIRDRVEAD VNISEDDAKA QALASEKVKA FTDGKNIVKV 

       850 
VYVKGKLVNI VVK 

« Hide

References

[1]"Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49526 / WA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001357 Genomic DNA. Translation: ACN84716.1.
RefSeqYP_002722420.1. NC_012225.1.

3D structure databases

ProteinModelPortalC0QWR3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING565034.BHWA1_02260.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACN84716; ACN84716; BHWA1_02260.
GeneID7667349.
KEGGbhy:BHWA1_02260.
PATRIC21182574. VBIBraHyo62857_2205.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBCLSK2810566.

Enzyme and pathway databases

BioCycBHYO565034:GJI7-2246-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_BRAHW
AccessionPrimary (citable) accession number: C0QWR3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries