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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Brachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei612 – 6121ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. leucine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. leucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-2246-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:BHWA1_02260
OrganismiBrachyspira hyodysenteriae (strain ATCC 49526 / WA1)
Taxonomic identifieri565034 [NCBI]
Taxonomic lineageiBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira
ProteomesiUP000001803 Componenti: Chromosome

Subcellular locationi

  1. Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 853853Leucine--tRNA ligasePRO_1000199183Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi565034.BHWA1_02260.

Structurei

3D structure databases

ProteinModelPortaliC0QWR3.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi40 – 5011"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0QWR3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEYNFTTIEK KWQKFWKDNQ SFKTVSKPTD KKYYVLEMFP YPSGKMHMGH
60 70 80 90 100
VSNYTIADSI ARYYKLLGYD ILHPMGWDAF GMPAENAAIE HKTHPAEWTL
110 120 130 140 150
KNIANMKDQL NLLGYSYDWD REVTTCLPDY YKWGQWFILK MYEKGLLYRK
160 170 180 190 200
GGDVNWCDHC NTVLANEQVT PEGTCWRCDG EVTKKKLEQW YIKVTDYAEQ
210 220 230 240 250
LDADLKLLEG YWPDNVIAMQ KNWIGRSVGA YINFNLDDGK AFPIFTTRPD
260 270 280 290 300
TIYGVTYMAI AWNYDGLLDM CTTEQKSAVE EFIKKSAKID QKTDYEKEGV
310 320 330 340 350
FTGRYVVNPF NGEKAPLYAA NFVLAEYGSG AVMAVPAHDQ RDFEFAKKYN
360 370 380 390 400
IPVKVVIQNA DNSLKAENMT EAYTEDGTVV NSDILNGLSS RDAIKRAIEY
410 420 430 440 450
ATEKGFGKEK VQYKLRDWLI SRQRYWGNPL PFVHCEKCGV VPVPESELPI
460 470 480 490 500
TLPMDIEFTV GDNPLKKSES FVNTTCPKCG GKARRETDTM DTFTCSSWYY
510 520 530 540 550
ARYTDAHNNQ MPFDPSAANA WLGVDQYIGG IEHACMHLLY SRFWYKFMRD
560 570 580 590 600
IGLVKGDEPF NRLLTQGMVL ANSYESRELK KFYTQEQMNN KEYEKDGIKK
610 620 630 640 650
EDIIVKMEKM SKSKANGIDP AEIIELFGAD AVRIFVMFVA PPEKDKEWSD
660 670 680 690 700
EGVKGSSRFL NRIWNLFLKY KDEEAFKNGK SFDYNNLSKE GQKLFRKYNK
710 720 730 740 750
TIKKVTIDIK DRFHFNTAIA ALMELLNDMS VIKLANNDDY AMFKEVIRGY
760 770 780 790 800
LILLNPIAPH MTEELYQILN FGKMILEERW VEHDEQYCKD DTFELVFQVN
810 820 830 840 850
GKIRDRVEAD VNISEDDAKA QALASEKVKA FTDGKNIVKV VYVKGKLVNI

VVK
Length:853
Mass (Da):98,617
Last modified:May 5, 2009 - v1
Checksum:iDB3FF4804951FD5C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN84716.1.
RefSeqiWP_012671748.1. NC_012225.1.
YP_002722420.1. NC_012225.1.

Genome annotation databases

EnsemblBacteriaiACN84716; ACN84716; BHWA1_02260.
KEGGibhy:BHWA1_02260.
PATRICi21182574. VBIBraHyo62857_2205.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001357 Genomic DNA. Translation: ACN84716.1.
RefSeqiWP_012671748.1. NC_012225.1.
YP_002722420.1. NC_012225.1.

3D structure databases

ProteinModelPortaliC0QWR3.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi565034.BHWA1_02260.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACN84716; ACN84716; BHWA1_02260.
KEGGibhy:BHWA1_02260.
PATRICi21182574. VBIBraHyo62857_2205.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiQSSWYFL.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciBHYO565034:GJI7-2246-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
PF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
    Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
    PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 49526 / WA1.

Entry informationi

Entry nameiSYL_BRAHW
AccessioniPrimary (citable) accession number: C0QWR3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: April 29, 2015
This is version 46 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.