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C0QWM9 (TYSY_BRAHW) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 36. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Thymidylate synthase

Short name=TS
Short name=TSase
EC=2.1.1.45
Gene names
Name:thyA
Ordered Locus Names:BHWA1_00171
OrganismBrachyspira hyodysenteriae (strain ATCC 49526 / WA1) [Complete proteome] [HAMAP]
Taxonomic identifier565034 [NCBI]
Taxonomic lineageBacteriaSpirochaetesSpirochaetalesBrachyspiraceaeBrachyspira

Protein attributes

Sequence length266 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Provides the sole de novo source of dTMP for DNA biosynthesis By similarity. HAMAP-Rule MF_00008

Catalytic activity

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP. HAMAP-Rule MF_00008

Pathway

Pyrimidine metabolism; dTTP biosynthesis. HAMAP-Rule MF_00008

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00008

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00008.

Sequence similarities

Belongs to the thymidylate synthase family. Bacterial-type ThyA subfamily.

Ontologies

Keywords
   Biological processNucleotide biosynthesis
   Cellular componentCytoplasm
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processdTMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-HAMAP

dTTP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionthymidylate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 266266Thymidylate synthase HAMAP-Rule MF_00008
PRO_1000197233

Sites

Active site1471 By similarity

Sequences

Sequence LengthMass (Da)Tools
C0QWM9 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 73D9BCC21F8D76C5

FASTA26630,799
        10         20         30         40         50         60 
MQNYLDLLKK VLEEGEETKD RTGVGTRRIF GPQLRFKFEG NKIPIITTKR VFMKGVIIEL 

        70         80         90        100        110        120 
LWFLQGSTNI KFLLENNVHI WDEWADDMGE LGPVYGKQWR AWETKEGNKI DQISNIVNTL 

       130        140        150        160        170        180 
RNNPASRRII LNAWNVGEID QMHLPPCHMM CQFSVNSKGG IITHLYQRSA DLFLGVPFNI 

       190        200        210        220        230        240 
SSYAILTRLL AMHSGLHASE LIMTFGDAHI YNNHIEQVKL QLSREPLEQT TELFIDNRPN 

       250        260 
IFSHVYEDFK LEGYKYYPTI KAEVAV 

« Hide

References

[1]"Genome sequence of the pathogenic intestinal spirochete Brachyspira hyodysenteriae reveals adaptations to its lifestyle in the porcine large intestine."
Bellgard M.I., Wanchanthuek P., La T., Ryan K., Moolhuijzen P., Albertyn Z., Shaban B., Motro Y., Dunn D.S., Schibeci D., Hunter A., Barrero R., Phillips N.D., Hampson D.J.
PLoS ONE 4:E4641-E4641(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 49526 / WA1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001357 Genomic DNA. Translation: ACN82671.1.
RefSeqYP_002720344.1. NC_012225.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING565034.BHWA1_00171.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACN82671; ACN82671; BHWA1_00171.
GeneID7665278.
KEGGbhy:BHWA1_00171.
PATRIC21178433. VBIBraHyo62857_0165.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0207.
HOGENOMHOG000257899.
KOK00560.
OMAYESHEGI.
OrthoDBEOG6K6V53.

Enzyme and pathway databases

BioCycBHYO565034:GJI7-171-MONOMER.
UniPathwayUPA00575.

Family and domain databases

Gene3D3.30.572.10. 1 hit.
HAMAPMF_00008. Thymidy_synth_bact.
InterProIPR023451. Thymidate_synth/dCMP_Mease.
IPR000398. Thymidylate_synthase.
IPR020940. Thymidylate_synthase_AS.
[Graphical view]
PfamPF00303. Thymidylat_synt. 1 hit.
[Graphical view]
PRINTSPR00108. THYMDSNTHASE.
SUPFAMSSF55831. SSF55831. 1 hit.
TIGRFAMsTIGR03284. thym_sym. 1 hit.
PROSITEPS00091. THYMIDYLATE_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameTYSY_BRAHW
AccessionPrimary (citable) accession number: C0QWM9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: May 14, 2014
This is version 36 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways