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C0QR28

- BIOB_PERMH

UniProt

C0QR28 - BIOB_PERMH

Protein

Biotin synthase

Gene

bioB

Organism
Persephonella marina (strain DSM 14350 / EX-H1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 40 (01 Oct 2014)
      Sequence version 1 (05 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

    Catalytic activityi

    Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
    Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi66 – 661Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi70 – 701Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi110 – 1101Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi142 – 1421Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi202 – 2021Iron-sulfur 2 (2Fe-2S)UniRule annotation
    Metal bindingi272 – 2721Iron-sulfur 2 (2Fe-2S)UniRule annotation

    GO - Molecular functioni

    1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
    2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    3. biotin synthase activity Source: UniProtKB-HAMAP
    4. iron ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. biotin biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Biotin biosynthesis

    Keywords - Ligandi

    2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    BioCyciPMAR123214:GIZP-1354-MONOMER.
    UniPathwayiUPA00078; UER00162.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
    Gene namesi
    Name:bioBUniRule annotation
    Ordered Locus Names:PERMA_1355
    OrganismiPersephonella marina (strain DSM 14350 / EX-H1)
    Taxonomic identifieri123214 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaePersephonella
    ProteomesiUP000001366: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 336336Biotin synthasePRO_0000381524Add
    BLAST

    Proteomic databases

    PRIDEiC0QR28.

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi123214.PERMA_1355.

    Structurei

    3D structure databases

    ProteinModelPortaliC0QR28.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0502.
    HOGENOMiHOG000239958.
    KOiK01012.
    OMAiCKEDCIF.
    OrthoDBiEOG622PMP.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_01694. BioB.
    InterProiIPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view]
    PfamiPF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001619. Biotin_synth. 1 hit.
    SMARTiSM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00433. bioB. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    C0QR28-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEDFIKGLAE RVLEGEKLSK EDGLKILSIP DEYLDLLVQE ASKVREAVFG    50
    DEVEFCSLIN AKNGACSEDC SFCAQSSKYP TPINAYGLVP KKELVEGAQK 100
    AVSIKANRYC IVTSGKRATK EEVEQIADAV REITENLPVR VCVSIGTVDE 150
    EDLKLLKDSG VDRVNHNLET SERHFKNIVT THSYKERLET IKTVQKVGLS 200
    TCTGGIFGIG ETDEDIVDLA SVYRELNVNS IPMNFLIPIP GTPLEGNKQL 250
    TPQRCLKIIS LFKLFNPQAE LRLCGGREIN LREYHDTAME IANCVMAGGY 300
    LTRAGRAPGK DEEMVRRLGR KLITGRDLFS KYKTAV 336
    Length:336
    Mass (Da):37,308
    Last modified:May 5, 2009 - v1
    Checksum:i8ADF65010B4CE95A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001230 Genomic DNA. Translation: ACO03951.1.
    RefSeqiYP_002731124.1. NC_012440.1.

    Genome annotation databases

    EnsemblBacteriaiACO03951; ACO03951; PERMA_1355.
    GeneIDi7675419.
    KEGGipmx:PERMA_1355.
    PATRICi22916167. VBIPerMar119911_1373.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001230 Genomic DNA. Translation: ACO03951.1 .
    RefSeqi YP_002731124.1. NC_012440.1.

    3D structure databases

    ProteinModelPortali C0QR28.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 123214.PERMA_1355.

    Proteomic databases

    PRIDEi C0QR28.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACO03951 ; ACO03951 ; PERMA_1355 .
    GeneIDi 7675419.
    KEGGi pmx:PERMA_1355.
    PATRICi 22916167. VBIPerMar119911_1373.

    Phylogenomic databases

    eggNOGi COG0502.
    HOGENOMi HOG000239958.
    KOi K01012.
    OMAi CKEDCIF.
    OrthoDBi EOG622PMP.

    Enzyme and pathway databases

    UniPathwayi UPA00078 ; UER00162 .
    BioCyci PMAR123214:GIZP-1354-MONOMER.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_01694. BioB.
    InterProi IPR013785. Aldolase_TIM.
    IPR010722. BATS_dom.
    IPR002684. Biotin_synth/BioAB.
    IPR024177. Biotin_synthase.
    IPR006638. Elp3/MiaB/NifB.
    IPR007197. rSAM.
    [Graphical view ]
    Pfami PF06968. BATS. 1 hit.
    PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001619. Biotin_synth. 1 hit.
    SMARTi SM00876. BATS. 1 hit.
    SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00433. bioB. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14350 / EX-H1.

    Entry informationi

    Entry nameiBIOB_PERMH
    AccessioniPrimary (citable) accession number: C0QR28
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 40 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3