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C0QPU7 (C0QPU7_PERMH) Unreviewed, UniProtKB/TrEMBL

Last modified May 1, 2013. Version 30. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Lipoyl synthase HAMAP-Rule MF_00206
EC=2.8.1.8 HAMAP-Rule MF_00206
Alternative name(s):
Lip-syn HAMAP-Rule MF_00206
Lipoate synthase HAMAP-Rule MF_00206
Lipoic acid synthase HAMAP-Rule MF_00206
Sulfur insertion protein LipA HAMAP-Rule MF_00206
Gene names
Name:lipA HAMAP-Rule MF_00206 EMBL ACO04008.1
Ordered Locus Names:PERMA_0907 EMBL ACO04008.1
OrganismPersephonella marina (strain DSM 14350 / EX-H1) [Complete proteome] [HAMAP] EMBL ACO04008.1
Taxonomic identifier123214 [NCBI]
Taxonomic lineageBacteria › Aquificae › Aquificales › Hydrogenothermaceae › Persephonella ›

Protein attributes

Sequence length280 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_00206

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_00206

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity. HAMAP-Rule MF_00206

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_00206

Subcellular location

Cytoplasm By similarity SAAS SAAS003698 HAMAP-Rule MF_00206.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family. HAMAP-Rule MF_00206

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Sites

Metal binding291Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding341Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding401Iron-sulfur 1 (4Fe-4S) By similarity HAMAP-Rule MF_00206
Metal binding551Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding591Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206
Metal binding621Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity HAMAP-Rule MF_00206

Sequences

Sequence LengthMass (Da)Tools
C0QPU7 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: DE6C426B91A47B4C

FASTA28031,840
        10         20         30         40         50         60 
MRPKVISPLI PEVTEMKKML RRLSLNTVCE EASCPNIGEC FKKKTATFMI LGNICTRACP 

        70         80         90        100        110        120 
YCDVDYGKPE PPDPEEPQNI ATAVKRLKLR HVVITSVTRD DLPDGGASHF AEVVKAVRRT 

       130        140        150        160        170        180 
APECRIEVLI PDFSGDRKSL EKVVEVKPDI INHNIETVPQ LYKKIRHKGD YKRSLEILRW 

       190        200        210        220        230        240 
VKEMDSSVIT KSGIMVGLGE SVEQVLSVMR DLVSVGCDIF TVGQYLQPSI HNYPVKKYYT 

       250        260        270        280 
EDEFRMFETE GYRIGFKEVY SGILVRSSYN ASEVFERLIS

« Hide

References

[1]"Complete and draft genome sequences of six members of the Aquificales."
Reysenbach A.L., Hamamura N., Podar M., Griffiths E., Ferreira S., Hochstein R., Heidelberg J., Johnson J., Mead D., Pohorille A., Sarmiento M., Schweighofer K., Seshadri R., Voytek M.A.
J. Bacteriol. 191:1992-1993(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 14350 / EX-H1.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		CP001230 Genomic DNA. Translation: ACO04008.1.
RefSeqYP_002730693.1. NC_012440.1.

3D structure databases

ProteinModelPortalC0QPU7.
ModBaseSearch...

Protein-protein interaction databases

STRING123214.PERMA_0907.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACO04008; ACO04008; PERMA_0907.
GeneID7675879.
KEGGpmx:PERMA_0907.
PATRIC22915279. VBIPerMar119911_0947.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0320.
HOGENOMHOG000235997.
KOK03644.
OMAEEYVTPE.
ProtClustDBPRK05481.

Enzyme and pathway databases

BioCycPMAR123214:GIZP-905-MONOMER.
UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameC0QPU7_PERMH
AccessionPrimary (citable) accession number: C0QPU7
Entry history
Integrated into UniProtKB/TrEMBL: May 5, 2009
Last sequence update: May 5, 2009
Last modified: May 1, 2013
This is version 30 of the entry and version 1 of the sequence. [Complete history]
Entry statusUnreviewed (UniProtKB/TrEMBL)
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info