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C0QP76

- SYI_PERMH

UniProt

C0QP76 - SYI_PERMH

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Persephonella marina (strain DSM 14350 / EX-H1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 41 (01 Oct 2014)
      Sequence version 1 (05 May 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
    Binding sitei612 – 6121ATPUniRule annotation
    Metal bindingi908 – 9081ZincUniRule annotation
    Metal bindingi911 – 9111ZincUniRule annotation
    Metal bindingi926 – 9261ZincUniRule annotation
    Metal bindingi929 – 9291ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciPMAR123214:GIZP-683-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:PERMA_0684
    OrganismiPersephonella marina (strain DSM 14350 / EX-H1)
    Taxonomic identifieri123214 [NCBI]
    Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaePersephonella
    ProteomesiUP000001366: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 947947Isoleucine--tRNA ligasePRO_1000216243Add
    BLAST

    Proteomic databases

    PRIDEiC0QP76.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi123214.PERMA_0684.

    Structurei

    3D structure databases

    ProteinModelPortaliC0QP76.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi609 – 6135"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiKPVHWCL.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C0QP76-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEWKDTLNLP KTAFPMKGNL PNKEPEIIKK WEEIDLYKRL REERKGKEKY    50
    ILHDGPPYAN GNIHLGHALN KILKDILVKY ESMKGKDAPF VPGWDCHGLP 100
    IEQQVEKLLK KEKKRKEDLS KSEFRKLCRE YALKYVNIQR EEFKRLGIIG 150
    NWEKPYLTMR PSYQAQEIRE LGKIFRKGIA YRGKKPVYWC IYDKTAEAEA 200
    EVEYKEKKDP SIYVAFELVE SPFDIKEKVY AVIWTTTPWT LPANLGIMVN 250
    PDFDYLFLRS EDKVYIVAKD LLESFSEKTG IEGEVIKEVK GRELEFLEYR 300
    HPFIDRVSKI YLSEFVELGT GTGLVHMAPG HGQEDYIIGQ RYGVEAFAPV 350
    DDEGRFTDEA PEFIQGLRVF EANERIVEKL RENGVLLHHE TVKHSYPHCW 400
    RCKNPVIFRA TPQWFISMDG ITEKGETLRG EALKEIERVK WIPHWGENRI 450
    KSMIENRPDW CISRQRSWGV PIAVFYCKRC GNIIDDEKVF EHIADLVEKD 500
    EFGADIWFER EAEELLPEGY RCPKCDGEEF KKEEDILDVW FDSGVSHASV 550
    LKSGFWDELK WPADMYLEGS DQHRGWFQSS LLEGVASYGR APYDAVLTHG 600
    FILDEKGNKM SKSLGNVIPP EKIIKMYGAD ILRLWVVSED YTEDIKIGMN 650
    LLKSIADDYR KIRNTFRYFL GNLYDFDANK DRVPYENLLE IDRWMLSKLQ 700
    RLIDRSHSAY SNYRFHKIYH EIKRFVIVDL SAVYLDILKD RLYVYAPDSL 750
    ERRSAQTVLY ELLDSLTKLL APILSFTTEE IWGYVREINP SVKESIHLEE 800
    MPVVNQDYID PELEETYEKL MKVRDDILKA LEEARRSDII RHPYEAKVVL 850
    SLPDEYRSVV EKRIDWIKFF FTVSQVELSD QAEGDVIIGG EKVEGGKIAV 900
    KKASGEKCPR CWIYDESVGK DGQPVCDRCM EQLERMEIKI SDIEEAK 947
    Length:947
    Mass (Da):110,771
    Last modified:May 5, 2009 - v1
    Checksum:iDE47D4F7B6D5933A
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001230 Genomic DNA. Translation: ACO03207.1.
    RefSeqiYP_002730472.1. NC_012440.1.

    Genome annotation databases

    EnsemblBacteriaiACO03207; ACO03207; PERMA_0684.
    GeneIDi7674544.
    KEGGipmx:PERMA_0684.
    PATRICi22914835. VBIPerMar119911_0726.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001230 Genomic DNA. Translation: ACO03207.1 .
    RefSeqi YP_002730472.1. NC_012440.1.

    3D structure databases

    ProteinModelPortali C0QP76.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 123214.PERMA_0684.

    Proteomic databases

    PRIDEi C0QP76.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACO03207 ; ACO03207 ; PERMA_0684 .
    GeneIDi 7674544.
    KEGGi pmx:PERMA_0684.
    PATRICi 22914835. VBIPerMar119911_0726.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi KPVHWCL.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci PMAR123214:GIZP-683-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 14350 / EX-H1.

    Entry informationi

    Entry nameiSYI_PERMH
    AccessioniPrimary (citable) accession number: C0QP76
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 22, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 41 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3