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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Persephonella marina (strain DSM 14350 / EX-H1)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei568Aminoacyl-adenylateUniRule annotation1
Binding sitei612ATPUniRule annotation1
Metal bindingi908ZincUniRule annotation1
Metal bindingi911ZincUniRule annotation1
Metal bindingi926ZincUniRule annotation1
Metal bindingi929ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PERMA_0684
OrganismiPersephonella marina (strain DSM 14350 / EX-H1)
Taxonomic identifieri123214 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaePersephonella
Proteomesi
  • UP000001366 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10002162431 – 947Isoleucine--tRNA ligaseAdd BLAST947

Proteomic databases

PRIDEiC0QP76

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi123214.PERMA_0684

Structurei

3D structure databases

ProteinModelPortaliC0QP76
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi609 – 613"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

C0QP76-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEWKDTLNLP KTAFPMKGNL PNKEPEIIKK WEEIDLYKRL REERKGKEKY
60 70 80 90 100
ILHDGPPYAN GNIHLGHALN KILKDILVKY ESMKGKDAPF VPGWDCHGLP
110 120 130 140 150
IEQQVEKLLK KEKKRKEDLS KSEFRKLCRE YALKYVNIQR EEFKRLGIIG
160 170 180 190 200
NWEKPYLTMR PSYQAQEIRE LGKIFRKGIA YRGKKPVYWC IYDKTAEAEA
210 220 230 240 250
EVEYKEKKDP SIYVAFELVE SPFDIKEKVY AVIWTTTPWT LPANLGIMVN
260 270 280 290 300
PDFDYLFLRS EDKVYIVAKD LLESFSEKTG IEGEVIKEVK GRELEFLEYR
310 320 330 340 350
HPFIDRVSKI YLSEFVELGT GTGLVHMAPG HGQEDYIIGQ RYGVEAFAPV
360 370 380 390 400
DDEGRFTDEA PEFIQGLRVF EANERIVEKL RENGVLLHHE TVKHSYPHCW
410 420 430 440 450
RCKNPVIFRA TPQWFISMDG ITEKGETLRG EALKEIERVK WIPHWGENRI
460 470 480 490 500
KSMIENRPDW CISRQRSWGV PIAVFYCKRC GNIIDDEKVF EHIADLVEKD
510 520 530 540 550
EFGADIWFER EAEELLPEGY RCPKCDGEEF KKEEDILDVW FDSGVSHASV
560 570 580 590 600
LKSGFWDELK WPADMYLEGS DQHRGWFQSS LLEGVASYGR APYDAVLTHG
610 620 630 640 650
FILDEKGNKM SKSLGNVIPP EKIIKMYGAD ILRLWVVSED YTEDIKIGMN
660 670 680 690 700
LLKSIADDYR KIRNTFRYFL GNLYDFDANK DRVPYENLLE IDRWMLSKLQ
710 720 730 740 750
RLIDRSHSAY SNYRFHKIYH EIKRFVIVDL SAVYLDILKD RLYVYAPDSL
760 770 780 790 800
ERRSAQTVLY ELLDSLTKLL APILSFTTEE IWGYVREINP SVKESIHLEE
810 820 830 840 850
MPVVNQDYID PELEETYEKL MKVRDDILKA LEEARRSDII RHPYEAKVVL
860 870 880 890 900
SLPDEYRSVV EKRIDWIKFF FTVSQVELSD QAEGDVIIGG EKVEGGKIAV
910 920 930 940
KKASGEKCPR CWIYDESVGK DGQPVCDRCM EQLERMEIKI SDIEEAK
Length:947
Mass (Da):110,771
Last modified:May 5, 2009 - v1
Checksum:iDE47D4F7B6D5933A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001230 Genomic DNA Translation: ACO03207.1

Genome annotation databases

EnsemblBacteriaiACO03207; ACO03207; PERMA_0684
KEGGipmx:PERMA_0684

Similar proteinsi

Entry informationi

Entry nameiSYI_PERMH
AccessioniPrimary (citable) accession number: C0QP76
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 5, 2009
Last modified: March 28, 2018
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

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