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C0QP76

- SYI_PERMH

UniProt

C0QP76 - SYI_PERMH

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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Persephonella marina (strain DSM 14350 / EX-H1)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei568 – 5681Aminoacyl-adenylateUniRule annotation
Binding sitei612 – 6121ATPUniRule annotation
Metal bindingi908 – 9081ZincUniRule annotation
Metal bindingi911 – 9111ZincUniRule annotation
Metal bindingi926 – 9261ZincUniRule annotation
Metal bindingi929 – 9291ZincUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciPMAR123214:GIZP-683-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:PERMA_0684
OrganismiPersephonella marina (strain DSM 14350 / EX-H1)
Taxonomic identifieri123214 [NCBI]
Taxonomic lineageiBacteriaAquificaeAquificalesHydrogenothermaceaePersephonella
ProteomesiUP000001366: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 947947Isoleucine--tRNA ligasePRO_1000216243Add
BLAST

Proteomic databases

PRIDEiC0QP76.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi123214.PERMA_0684.

Structurei

3D structure databases

ProteinModelPortaliC0QP76.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi609 – 6135"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiKPVHWCL.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0QP76-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEWKDTLNLP KTAFPMKGNL PNKEPEIIKK WEEIDLYKRL REERKGKEKY
60 70 80 90 100
ILHDGPPYAN GNIHLGHALN KILKDILVKY ESMKGKDAPF VPGWDCHGLP
110 120 130 140 150
IEQQVEKLLK KEKKRKEDLS KSEFRKLCRE YALKYVNIQR EEFKRLGIIG
160 170 180 190 200
NWEKPYLTMR PSYQAQEIRE LGKIFRKGIA YRGKKPVYWC IYDKTAEAEA
210 220 230 240 250
EVEYKEKKDP SIYVAFELVE SPFDIKEKVY AVIWTTTPWT LPANLGIMVN
260 270 280 290 300
PDFDYLFLRS EDKVYIVAKD LLESFSEKTG IEGEVIKEVK GRELEFLEYR
310 320 330 340 350
HPFIDRVSKI YLSEFVELGT GTGLVHMAPG HGQEDYIIGQ RYGVEAFAPV
360 370 380 390 400
DDEGRFTDEA PEFIQGLRVF EANERIVEKL RENGVLLHHE TVKHSYPHCW
410 420 430 440 450
RCKNPVIFRA TPQWFISMDG ITEKGETLRG EALKEIERVK WIPHWGENRI
460 470 480 490 500
KSMIENRPDW CISRQRSWGV PIAVFYCKRC GNIIDDEKVF EHIADLVEKD
510 520 530 540 550
EFGADIWFER EAEELLPEGY RCPKCDGEEF KKEEDILDVW FDSGVSHASV
560 570 580 590 600
LKSGFWDELK WPADMYLEGS DQHRGWFQSS LLEGVASYGR APYDAVLTHG
610 620 630 640 650
FILDEKGNKM SKSLGNVIPP EKIIKMYGAD ILRLWVVSED YTEDIKIGMN
660 670 680 690 700
LLKSIADDYR KIRNTFRYFL GNLYDFDANK DRVPYENLLE IDRWMLSKLQ
710 720 730 740 750
RLIDRSHSAY SNYRFHKIYH EIKRFVIVDL SAVYLDILKD RLYVYAPDSL
760 770 780 790 800
ERRSAQTVLY ELLDSLTKLL APILSFTTEE IWGYVREINP SVKESIHLEE
810 820 830 840 850
MPVVNQDYID PELEETYEKL MKVRDDILKA LEEARRSDII RHPYEAKVVL
860 870 880 890 900
SLPDEYRSVV EKRIDWIKFF FTVSQVELSD QAEGDVIIGG EKVEGGKIAV
910 920 930 940
KKASGEKCPR CWIYDESVGK DGQPVCDRCM EQLERMEIKI SDIEEAK
Length:947
Mass (Da):110,771
Last modified:May 5, 2009 - v1
Checksum:iDE47D4F7B6D5933A
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001230 Genomic DNA. Translation: ACO03207.1.
RefSeqiYP_002730472.1. NC_012440.1.

Genome annotation databases

EnsemblBacteriaiACO03207; ACO03207; PERMA_0684.
GeneIDi7674544.
KEGGipmx:PERMA_0684.
PATRICi22914835. VBIPerMar119911_0726.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001230 Genomic DNA. Translation: ACO03207.1 .
RefSeqi YP_002730472.1. NC_012440.1.

3D structure databases

ProteinModelPortali C0QP76.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 123214.PERMA_0684.

Proteomic databases

PRIDEi C0QP76.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACO03207 ; ACO03207 ; PERMA_0684 .
GeneIDi 7674544.
KEGGi pmx:PERMA_0684.
PATRICi 22914835. VBIPerMar119911_0726.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi KPVHWCL.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci PMAR123214:GIZP-683-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 14350 / EX-H1.

Entry informationi

Entry nameiSYI_PERMH
AccessioniPrimary (citable) accession number: C0QP76
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 5, 2009
Last modified: October 29, 2014
This is version 42 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3