C0QLF1 (PROA_DESAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Gamma-glutamyl phosphate reductase
Short name=GPR
EC=1.2.1.41

Alternative name(s):
Glutamate-5-semialdehyde dehydrogenase
Glutamyl-gamma-semialdehyde dehydrogenase
Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	HRM2_31740

Organism

Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) [Complete proteome] [HAMAP]

Taxonomic identifier

177437 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfobacterales › Desulfobacteraceae › Desulfobacterium › Desulfobacterium autotrophicum

Protein attributes

Sequence length	418 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate By similarity. HAMAP-Rule MF_00412
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP+ = L-glutamyl 5-phosphate + NADPH. HAMAP-Rule MF_00412
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2. HAMAP-Rule MF_00412
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_00412.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	L-proline biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 418	418	Gamma-glutamyl phosphate reductase HAMAP-Rule MF_00412		PRO_1000205992

Sequences

	Sequence		Length	Mass (Da)
	C0QLF1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 51CDA5FDD7AC0651	FASTA	418	45,025
	10 20 30 40 50 60 MTLASMIEDI ARRARTAARP LATASADTKN TVLAEIARGL AHGKREIEAE NQKDLKAARD 70 80 90 100 110 120 SGMSAAMIDR LTISDQTLES MIKGLNQVIA LPDPVGRITG AWTRPNGLEI SKRRIPLGVI 130 140 150 160 170 180 AMIYESRPNV TVDAAALCLK AGNAAILRGG SEAFFSNTIL ARIIGNALET VGISRDSVQV 190 200 210 220 230 240 LPVKDRQAIT ELLQQEAYID LVIPRGGESL IRFVVKHSTI PVLKHYKGVC HVYVDETCNM 250 260 270 280 290 300 DEAVDICINA KTQRPGVCNA METLLVHERI APLFLPRMAQ AFSAAKVEMR GCPATLAMVP 310 320 330 340 350 360 QALPADETDW STEYLDLIVS VKIVKDLAQA MAHIAVFGSD HTDVIVTDIP ENAETFINTV 370 380 390 400 410 SSSMVGVNVS TRFNDGGELG LGAEIGISTS RLHAFGPMGL EELTSTKFVV VGKGQTRQ

References

[1]

"Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide." Strittmatter A.W., Liesegang H., Rabus R., Decker I., Amann J., Andres S., Henne A., Fricke W.F., Martinez-Arias R., Bartels D., Goesmann A., Krause L., Puehler A., Klenk H.P., Richter M., Schuler M., Gloeckner F.O., Meyerdierks A., Gottschalk G., Amann R. Environ. Microbiol. 11:1038-1055(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43914 / DSM 3382 / HRM2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001087 Genomic DNA. Translation: ACN16255.1.
RefSeq	YP_002604419.1. NC_012108.1.
3D structure databases
ProteinModelPortal	C0QLF1.
ModBase	Search...
Protein-protein interaction databases
STRING	177437.HRM2_31740.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACN16255; ACN16255; HRM2_31740.
GeneID	7502638.
KEGG	dat:HRM2_31740.
PATRIC	21686057. VBIDesAut25181_3280.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0014.
HOGENOM	HOG000246356.
KO	K00147.
OMA	QRPNGMT.
Enzyme and pathway databases
BioCyc	DAUT177437:GHLR-3172-MONOMER.
UniPathway	UPA00098; UER00360.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 2 hits.
HAMAP	MF_00412. ProA.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. IPR000965. G-glutamylP_reductase. IPR020593. G-glutamylP_reductase_CS. IPR012134. Glu-5-SA_DH. [Graphical view]
PANTHER	PTHR11063:SF1. PTHR11063:SF1. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
SUPFAM	SSF53720. Aldehyde_DH/Histidinol_DH. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_DESAH

Accession

Primary (citable) accession number: C0QLF1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program