Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

C0QJ86

- DNLJ_DESAH

UniProt

C0QJ86 - DNLJ_DESAH

Protein

DNA ligase

Gene

ligA

Organism
Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 43 (01 Oct 2014)
      Sequence version 1 (05 May 2009)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

    Catalytic activityi

    NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

    Cofactori

    Magnesium or manganese.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei119 – 1191NADUniRule annotation
    Active sitei121 – 1211N6-AMP-lysine intermediateUniRule annotation
    Binding sitei142 – 1421NADUniRule annotation
    Binding sitei182 – 1821NADUniRule annotation
    Binding sitei302 – 3021NADUniRule annotation
    Binding sitei326 – 3261NADUniRule annotation
    Metal bindingi420 – 4201ZincUniRule annotation
    Metal bindingi423 – 4231ZincUniRule annotation
    Metal bindingi438 – 4381ZincUniRule annotation
    Metal bindingi443 – 4431ZincUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi39 – 435NADUniRule annotation
    Nucleotide bindingi88 – 892NADUniRule annotation

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA ligase (NAD+) activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. DNA repair Source: UniProtKB-KW
    2. DNA replication Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    DNA damage, DNA repair, DNA replication

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, NAD, Zinc

    Enzyme and pathway databases

    BioCyciDAUT177437:GHLR-2809-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
    Alternative name(s):
    Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
    Gene namesi
    Name:ligAUniRule annotation
    Ordered Locus Names:HRM2_28100
    OrganismiDesulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
    Taxonomic identifieri177437 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfobacterium
    ProteomesiUP000000442: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 685685DNA ligasePRO_0000380361Add
    BLAST

    Proteomic databases

    PRIDEiC0QJ86.

    Interactioni

    Protein-protein interaction databases

    STRINGi177437.HRM2_28100.

    Structurei

    3D structure databases

    ProteinModelPortaliC0QJ86.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini606 – 68580BRCTUniRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation
    Contains 1 BRCT domain.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0272.
    HOGENOMiHOG000218459.
    KOiK01972.
    OMAiKEYYVDS.
    OrthoDBiEOG6TTVM9.

    Family and domain databases

    Gene3Di2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    HAMAPiMF_01588. DNA_ligase_A.
    InterProiIPR001357. BRCT_dom.
    IPR018239. DNA_ligase_AS.
    IPR001679. DNAligase.
    IPR013839. DNAligase_adenylation.
    IPR013840. DNAligase_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR012340. NA-bd_OB-fold.
    IPR004150. NAD_DNA_ligase_OB.
    IPR010994. RuvA_2-like.
    IPR004149. Znf_DNAligase_C4.
    [Graphical view]
    PfamiPF00533. BRCT. 1 hit.
    PF01653. DNA_ligase_aden. 1 hit.
    PF03120. DNA_ligase_OB. 1 hit.
    PF03119. DNA_ligase_ZBD. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001604. LigA. 1 hit.
    SMARTiSM00292. BRCT. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00532. LIGANc. 1 hit.
    [Graphical view]
    SUPFAMiSSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsiTIGR00575. dnlj. 1 hit.
    PROSITEiPS50172. BRCT. 1 hit.
    PS01056. DNA_LIGASE_N2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    C0QJ86-1 [UniParc]FASTAAdd to Basket

    « Hide

    MIDPSSSFDR IKKEARLLRE QLHVHNINYH VKDDPVISDG EYDRMMQRLI    50
    AIETQFPELS TPDSPTRRIG AKALTAFETA EHAIPMQSLD NAFSDQDVID 100
    FHNRTAKILN TSDIRYTVEP KLDGVAVELR YEQGSLTLAL TRGDGTMGEV 150
    ITDNARTIPS VPLKLAPAGN GTIPDVLEVR GEVIINSKDF EGLNKKRLAT 200
    GEPLFANPRN AAAGSLRQLD SRVTAKRPLE IFVYGVGRAQ ELMANFDIGS 250
    HSALLESLKR LGFRINPLIR SGLSLTEVLD RFKAFETMRQ DLDYEIDGMV 300
    IKVDDIVFQE RLGTKARSPR WAIAYKFPAM EETTVINDII VQVGRTGTLT 350
    PVAILEPVNI GGVMVARASL HNQDEIQNKD IRINDTVLVK RAGDVIPKVV 400
    KPVTALRTGS ERVFVMPTHC PVCHSPVRRL DNEAAVKCIN ASCKAQLKQR 450
    LKHFVSKGGF DMEGLGTKLI DQLVDRTLVG SFADLFTLDR ETLAAMDRMG 500
    EKSATNIVQA IERSKRIPLK RFLFALGMAH TGESAAQLLS STFFTLEALL 550
    KASAEALGAI EGVGPKTADS VVAFFANPDN QETIARMMEN GVVIENHTVV 600
    ESAALDNATF FSEKRVVLTG TLGTLTRSEA KQRLEEQGAR VVSSVSKNTD 650
    ILVAGEASGS KLVKARSLGV TIMDEQTFLE HLDRG 685
    Length:685
    Mass (Da):75,284
    Last modified:May 5, 2009 - v1
    Checksum:i3B0C000032505C3C
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001087 Genomic DNA. Translation: ACN15899.1.
    RefSeqiWP_015904662.1. NC_012108.1.
    YP_002604063.1. NC_012108.1.

    Genome annotation databases

    EnsemblBacteriaiACN15899; ACN15899; HRM2_28100.
    GeneIDi7501340.
    KEGGidat:HRM2_28100.
    PATRICi21685323. VBIDesAut25181_2920.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP001087 Genomic DNA. Translation: ACN15899.1 .
    RefSeqi WP_015904662.1. NC_012108.1.
    YP_002604063.1. NC_012108.1.

    3D structure databases

    ProteinModelPortali C0QJ86.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 177437.HRM2_28100.

    Proteomic databases

    PRIDEi C0QJ86.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ACN15899 ; ACN15899 ; HRM2_28100 .
    GeneIDi 7501340.
    KEGGi dat:HRM2_28100.
    PATRICi 21685323. VBIDesAut25181_2920.

    Phylogenomic databases

    eggNOGi COG0272.
    HOGENOMi HOG000218459.
    KOi K01972.
    OMAi KEYYVDS.
    OrthoDBi EOG6TTVM9.

    Enzyme and pathway databases

    BioCyci DAUT177437:GHLR-2809-MONOMER.

    Family and domain databases

    Gene3Di 2.40.50.140. 1 hit.
    3.40.50.10190. 1 hit.
    HAMAPi MF_01588. DNA_ligase_A.
    InterProi IPR001357. BRCT_dom.
    IPR018239. DNA_ligase_AS.
    IPR001679. DNAligase.
    IPR013839. DNAligase_adenylation.
    IPR013840. DNAligase_N.
    IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
    IPR012340. NA-bd_OB-fold.
    IPR004150. NAD_DNA_ligase_OB.
    IPR010994. RuvA_2-like.
    IPR004149. Znf_DNAligase_C4.
    [Graphical view ]
    Pfami PF00533. BRCT. 1 hit.
    PF01653. DNA_ligase_aden. 1 hit.
    PF03120. DNA_ligase_OB. 1 hit.
    PF03119. DNA_ligase_ZBD. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001604. LigA. 1 hit.
    SMARTi SM00292. BRCT. 1 hit.
    SM00278. HhH1. 2 hits.
    SM00532. LIGANc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF47781. SSF47781. 1 hit.
    SSF50249. SSF50249. 1 hit.
    SSF52113. SSF52113. 1 hit.
    TIGRFAMsi TIGR00575. dnlj. 1 hit.
    PROSITEi PS50172. BRCT. 1 hit.
    PS01056. DNA_LIGASE_N2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 43914 / DSM 3382 / HRM2.

    Entry informationi

    Entry nameiDNLJ_DESAH
    AccessioniPrimary (citable) accession number: C0QJ86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 28, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3