Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

C0QJ86

- DNLJ_DESAH

UniProt

C0QJ86 - DNLJ_DESAH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

DNA ligase

Gene

ligA

Organism
Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA.UniRule annotation

Catalytic activityi

NAD+ + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + beta-nicotinamide D-ribonucleotide + (deoxyribonucleotide)(n+m).UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei119 – 1191NADUniRule annotation
Active sitei121 – 1211N6-AMP-lysine intermediateUniRule annotation
Binding sitei142 – 1421NADUniRule annotation
Binding sitei182 – 1821NADUniRule annotation
Binding sitei302 – 3021NADUniRule annotation
Binding sitei326 – 3261NADUniRule annotation
Metal bindingi420 – 4201ZincUniRule annotation
Metal bindingi423 – 4231ZincUniRule annotation
Metal bindingi438 – 4381ZincUniRule annotation
Metal bindingi443 – 4431ZincUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi39 – 435NADUniRule annotation
Nucleotide bindingi88 – 892NADUniRule annotation

GO - Molecular functioni

  1. DNA binding Source: InterPro
  2. DNA ligase (NAD+) activity Source: UniProtKB-HAMAP
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. DNA repair Source: UniProtKB-KW
  2. DNA replication Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

DNA damage, DNA repair, DNA replication

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, NAD, Zinc

Enzyme and pathway databases

BioCyciDAUT177437:GHLR-2809-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA ligaseUniRule annotation (EC:6.5.1.2UniRule annotation)
Alternative name(s):
Polydeoxyribonucleotide synthase [NAD(+)]UniRule annotation
Gene namesi
Name:ligAUniRule annotation
Ordered Locus Names:HRM2_28100
OrganismiDesulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
Taxonomic identifieri177437 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfobacterium
ProteomesiUP000000442: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 685685DNA ligasePRO_0000380361Add
BLAST

Proteomic databases

PRIDEiC0QJ86.

Interactioni

Protein-protein interaction databases

STRINGi177437.HRM2_28100.

Structurei

3D structure databases

ProteinModelPortaliC0QJ86.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini606 – 68580BRCTUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the NAD-dependent DNA ligase family. LigA subfamily.UniRule annotation
Contains 1 BRCT domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG0272.
HOGENOMiHOG000218459.
KOiK01972.
OMAiKEYYVDS.
OrthoDBiEOG6TTVM9.

Family and domain databases

Gene3Di2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
HAMAPiMF_01588. DNA_ligase_A.
InterProiIPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR004150. NAD_DNA_ligase_OB.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view]
PfamiPF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view]
PIRSFiPIRSF001604. LigA. 1 hit.
SMARTiSM00292. BRCT. 1 hit.
SM00278. HhH1. 2 hits.
SM00532. LIGANc. 1 hit.
[Graphical view]
SUPFAMiSSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsiTIGR00575. dnlj. 1 hit.
PROSITEiPS50172. BRCT. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0QJ86-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MIDPSSSFDR IKKEARLLRE QLHVHNINYH VKDDPVISDG EYDRMMQRLI
60 70 80 90 100
AIETQFPELS TPDSPTRRIG AKALTAFETA EHAIPMQSLD NAFSDQDVID
110 120 130 140 150
FHNRTAKILN TSDIRYTVEP KLDGVAVELR YEQGSLTLAL TRGDGTMGEV
160 170 180 190 200
ITDNARTIPS VPLKLAPAGN GTIPDVLEVR GEVIINSKDF EGLNKKRLAT
210 220 230 240 250
GEPLFANPRN AAAGSLRQLD SRVTAKRPLE IFVYGVGRAQ ELMANFDIGS
260 270 280 290 300
HSALLESLKR LGFRINPLIR SGLSLTEVLD RFKAFETMRQ DLDYEIDGMV
310 320 330 340 350
IKVDDIVFQE RLGTKARSPR WAIAYKFPAM EETTVINDII VQVGRTGTLT
360 370 380 390 400
PVAILEPVNI GGVMVARASL HNQDEIQNKD IRINDTVLVK RAGDVIPKVV
410 420 430 440 450
KPVTALRTGS ERVFVMPTHC PVCHSPVRRL DNEAAVKCIN ASCKAQLKQR
460 470 480 490 500
LKHFVSKGGF DMEGLGTKLI DQLVDRTLVG SFADLFTLDR ETLAAMDRMG
510 520 530 540 550
EKSATNIVQA IERSKRIPLK RFLFALGMAH TGESAAQLLS STFFTLEALL
560 570 580 590 600
KASAEALGAI EGVGPKTADS VVAFFANPDN QETIARMMEN GVVIENHTVV
610 620 630 640 650
ESAALDNATF FSEKRVVLTG TLGTLTRSEA KQRLEEQGAR VVSSVSKNTD
660 670 680
ILVAGEASGS KLVKARSLGV TIMDEQTFLE HLDRG
Length:685
Mass (Da):75,284
Last modified:May 5, 2009 - v1
Checksum:i3B0C000032505C3C
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001087 Genomic DNA. Translation: ACN15899.1.
RefSeqiWP_015904662.1. NC_012108.1.
YP_002604063.1. NC_012108.1.

Genome annotation databases

EnsemblBacteriaiACN15899; ACN15899; HRM2_28100.
GeneIDi7501340.
KEGGidat:HRM2_28100.
PATRICi21685323. VBIDesAut25181_2920.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP001087 Genomic DNA. Translation: ACN15899.1 .
RefSeqi WP_015904662.1. NC_012108.1.
YP_002604063.1. NC_012108.1.

3D structure databases

ProteinModelPortali C0QJ86.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 177437.HRM2_28100.

Proteomic databases

PRIDEi C0QJ86.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ACN15899 ; ACN15899 ; HRM2_28100 .
GeneIDi 7501340.
KEGGi dat:HRM2_28100.
PATRICi 21685323. VBIDesAut25181_2920.

Phylogenomic databases

eggNOGi COG0272.
HOGENOMi HOG000218459.
KOi K01972.
OMAi KEYYVDS.
OrthoDBi EOG6TTVM9.

Enzyme and pathway databases

BioCyci DAUT177437:GHLR-2809-MONOMER.

Family and domain databases

Gene3Di 2.40.50.140. 1 hit.
3.40.50.10190. 1 hit.
HAMAPi MF_01588. DNA_ligase_A.
InterProi IPR001357. BRCT_dom.
IPR018239. DNA_ligase_AS.
IPR001679. DNAligase.
IPR013839. DNAligase_adenylation.
IPR013840. DNAligase_N.
IPR003583. Hlx-hairpin-Hlx_DNA-bd_motif.
IPR012340. NA-bd_OB-fold.
IPR004150. NAD_DNA_ligase_OB.
IPR010994. RuvA_2-like.
IPR004149. Znf_DNAligase_C4.
[Graphical view ]
Pfami PF00533. BRCT. 1 hit.
PF01653. DNA_ligase_aden. 1 hit.
PF03120. DNA_ligase_OB. 1 hit.
PF03119. DNA_ligase_ZBD. 1 hit.
[Graphical view ]
PIRSFi PIRSF001604. LigA. 1 hit.
SMARTi SM00292. BRCT. 1 hit.
SM00278. HhH1. 2 hits.
SM00532. LIGANc. 1 hit.
[Graphical view ]
SUPFAMi SSF47781. SSF47781. 1 hit.
SSF50249. SSF50249. 1 hit.
SSF52113. SSF52113. 1 hit.
TIGRFAMsi TIGR00575. dnlj. 1 hit.
PROSITEi PS50172. BRCT. 1 hit.
PS01056. DNA_LIGASE_N2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43914 / DSM 3382 / HRM2.

Entry informationi

Entry nameiDNLJ_DESAH
AccessioniPrimary (citable) accession number: C0QJ86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: May 5, 2009
Last modified: October 1, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3