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C0QI75 (SYL_DESAH) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:HRM2_27190
OrganismDesulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) [Complete proteome] [HAMAP]
Taxonomic identifier177437 [NCBI]
Taxonomic lineageBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfobacterium

Protein attributes

Sequence length863 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 863863Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_1000202217

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif618 – 6225"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site6211ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
C0QI75 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 1908E67EA74F6894

FASTA86398,680
        10         20         30         40         50         60 
MEERYNPEKV EPLWQAYWNQ HQTFKATEDG SKPKYYLLEM FPYPSGKIHM GHVRNYTIGD 

        70         80         90        100        110        120 
VVVRYKRMKG FNVLHPMGWD AFGMPAENAA IDNNTHPAAW TYENIRTMRR QLKRMGFSYD 

       130        140        150        160        170        180 
WDREIATCRP EYYRWEQWLF LKMLDKDMVY RKESYVNWCD HCQTVLANEQ VEQDMCWRCG 

       190        200        210        220        230        240 
KPVQQKKLWQ WFFRITDFAE DLLVHCDKLP GWPDNVTLMQ KNWIGKSQGS EIRFPIQGID 

       250        260        270        280        290        300 
ENIPVFTTRP DTLFGSTFMC LAPEHPLVET LSRGTDQAAA VTEFTTRVLN QERSSIALEK 

       310        320        330        340        350        360 
YEKEGVFTGA WCINPVTREK MPIYTANFAL MEYGTGAVMS VPAHDQRDFD FARKYGLPIK 

       370        380        390        400        410        420 
VVIQPPGEPL DPATMIEAYT GQGTLADSGE FSGLGNLEAM GAITRWLENK GLGKTTVSFR 

       430        440        450        460        470        480 
LRDWGISRQR YWGTPIPVIH CPDCGIVPVK EETLPVVLPE DAALLDNGGS PLATLDGFAR 

       490        500        510        520        530        540 
VNCPVCNRAD ARRETDTMDT FVESSWYFAR YCSPRYDKGM FDPAAVAYWM PVDQYIGGVE 

       550        560        570        580        590        600 
HAILHLLYSR YFMRVLNTLG LIDFKEPFER LLTQGMVCKE TLTCPEHGFI YPDDAETVND 

       610        620        630        640        650        660 
KVICKRCNSD VEVGRVIKMS KSKKNVIDPN ALLDQYGADI TRLFCLFAAP PEKDLEWNDD 

       670        680        690        700        710        720 
GVEGCNRFIN RVWRLADRCK STYIDLLPYS GVVADLKGEP KKLFIKANQT IKKVTDDIEE 

       730        740        750        760        770        780 
SFHFNTAISA VMELVNAMYS ADLDPDQTDM GEVALFCIEN IVLLLSPIVP HFCEELWSIL 

       790        800        810        820        830        840 
GHGPSIVDQP WPDYQKDALL TDEILIVVQV NGKLRSKFSV DAAVSDDFIR KAALADEQVE 

       850        860 
KYIKGKTIKK VIVVKKKLVN IVV 

« Hide

References

[1]"Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide."
Strittmatter A.W., Liesegang H., Rabus R., Decker I., Amann J., Andres S., Henne A., Fricke W.F., Martinez-Arias R., Bartels D., Goesmann A., Krause L., Puehler A., Klenk H.P., Richter M., Schuler M., Gloeckner F.O., Meyerdierks A., Gottschalk G., Amann R.
Environ. Microbiol. 11:1038-1055(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 43914 / DSM 3382 / HRM2.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP001087 Genomic DNA. Translation: ACN15811.1.
RefSeqYP_002603975.1. NC_012108.1.

3D structure databases

ProteinModelPortalC0QI75.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING177437.HRM2_27190.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaACN15811; ACN15811; HRM2_27190.
GeneID7501492.
KEGGdat:HRM2_27190.
PATRIC21685143. VBIDesAut25181_2833.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycDAUT177437:GHLR-2718-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 3 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 3 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_DESAH
AccessionPrimary (citable) accession number: C0QI75
Entry history
Integrated into UniProtKB/Swiss-Prot: September 22, 2009
Last sequence update: May 5, 2009
Last modified: April 16, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries