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Protein

Polyribonucleotide nucleotidyltransferase

Gene

pnp

Organism
Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in mRNA degradation. Catalyzes the phosphorolysis of single-stranded polyribonucleotides processively in the 3'- to 5'-direction.UniRule annotation

Catalytic activityi

RNA(n+1) + phosphate = RNA(n) + a nucleoside diphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi484 – 4841MagnesiumUniRule annotation
Metal bindingi490 – 4901MagnesiumUniRule annotation

GO - Molecular functioni

  1. 3'-5'-exoribonuclease activity Source: InterPro
  2. magnesium ion binding Source: UniProtKB-HAMAP
  3. polyribonucleotide nucleotidyltransferase activity Source: UniProtKB-HAMAP
  4. RNA binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. mRNA catabolic process Source: UniProtKB-HAMAP
  2. RNA processing Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Nucleotidyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding, RNA-binding

Enzyme and pathway databases

BioCyciDAUT177437:GHLR-470-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Polyribonucleotide nucleotidyltransferaseUniRule annotation (EC:2.7.7.8UniRule annotation)
Alternative name(s):
Polynucleotide phosphorylaseUniRule annotation
Short name:
PNPaseUniRule annotation
Gene namesi
Name:pnpUniRule annotation
Ordered Locus Names:HRM2_04700
OrganismiDesulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)
Taxonomic identifieri177437 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaDeltaproteobacteriaDesulfobacteralesDesulfobacteraceaeDesulfobacterium
ProteomesiUP000000442: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 695695Polyribonucleotide nucleotidyltransferasePRO_0000381883Add
BLAST

Proteomic databases

PRIDEiC0QHM6.

Interactioni

Protein-protein interaction databases

STRINGi177437.HRM2_04700.

Structurei

3D structure databases

ProteinModelPortaliC0QHM6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini551 – 61060KHUniRule annotationAdd
BLAST
Domaini620 – 68869S1 motifUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the polyribonucleotide nucleotidyltransferase family.UniRule annotation
Contains 1 KH domain.UniRule annotation
Contains 1 S1 motif domain.UniRule annotation

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0QHM6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEQVFKTELN GKEFSLRAGK IAKQASGSVI VQYGETIVLV TAVAAKEARD
60 70 80 90 100
SVSFLPLTVE YQEKIYAAGR IPGNYFRREI GRPSEKETLT ARLIDRPIRP
110 120 130 140 150
LFEKGWASEI QVIATVISTD KENDPDVLAL VGASAALQMS DIPFAGPIAG
160 170 180 190 200
LRVGRIDGKF IANPTVQEME NSDIELVVAG SKTGVVMVEG GADVVSEADM
210 220 230 240 250
LEAIFFGHQA MQPLIQLQED LKKALGKEKR AYTPPLVDEE LMAKVVELAQ
260 270 280 290 300
DKIHTAVQTQ GKFERVKALD DVRTEVIETL GEAFADRINE IKSMFGDLVK
310 320 330 340 350
KVSRKIVLTE NQRIDGRAFD EVREITCEVG VLPRPHGSAL FTRGETQVLG
360 370 380 390 400
VLTLGSGPDE QRIETLNGNE TRNFMLHYNF PPFSVGECRR AGGPSRRDVG
410 420 430 440 450
HGNLAHRALA RVLPDQADFE YTIRLVGEVM ESNGSSSMGT VCAGTLALMD
460 470 480 490 500
GGVPIAAPVS GIAMGLVSDA DNTVVLTDIL GDEDHFGDMD FKVAGTEQGI
510 520 530 540 550
TALQMDIKIR ELPKAVLVKA LDQARIGRLH ILKRMLATLE IHRDKMSPYA
560 570 580 590 600
PKIITVKINP DKIREIIGPG GKMIRSIQAD TNTNIEVDDS GLIKIAAENQ
610 620 630 640 650
EDGEAAAKIV SDIGMDPEIG GIYEGEVVKT TDFGAFVRIK TGTDGLVHIS
660 670 680 690
ELANHRVKKV TDVVKEGEVI RVKVLEITRD GKIRLSCKAL EEEEK
Length:695
Mass (Da):75,737
Last modified:May 5, 2009 - v1
Checksum:i492B170347DA21A2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001087 Genomic DNA. Translation: ACN13584.1.
RefSeqiWP_012662833.1. NC_012108.1.
YP_002601748.1. NC_012108.1.

Genome annotation databases

EnsemblBacteriaiACN13584; ACN13584; HRM2_04700.
GeneIDi7503273.
KEGGidat:HRM2_04700.
PATRICi21680421. VBIDesAut25181_0493.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP001087 Genomic DNA. Translation: ACN13584.1.
RefSeqiWP_012662833.1. NC_012108.1.
YP_002601748.1. NC_012108.1.

3D structure databases

ProteinModelPortaliC0QHM6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi177437.HRM2_04700.

Proteomic databases

PRIDEiC0QHM6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiACN13584; ACN13584; HRM2_04700.
GeneIDi7503273.
KEGGidat:HRM2_04700.
PATRICi21680421. VBIDesAut25181_0493.

Phylogenomic databases

eggNOGiCOG1185.
HOGENOMiHOG000218327.
KOiK00962.
OMAiRFMFHYN.
OrthoDBiEOG6WT8CC.

Enzyme and pathway databases

BioCyciDAUT177437:GHLR-470-MONOMER.

Family and domain databases

Gene3Di1.10.10.400. 1 hit.
2.40.50.140. 1 hit.
3.30.1370.10. 1 hit.
3.30.230.70. 2 hits.
HAMAPiMF_01595. PNPase.
InterProiIPR001247. ExoRNase_PH_dom1.
IPR015847. ExoRNase_PH_dom2.
IPR004087. KH_dom.
IPR004088. KH_dom_type_1.
IPR012340. NA-bd_OB-fold.
IPR012162. PNPase.
IPR027408. PNPase/RNase_PH_dom.
IPR015848. PNPase_PH_RNA-bd_bac/org-type.
IPR003029. Rbsml_prot_S1_RNA-bd_dom.
IPR020568. Ribosomal_S5_D2-typ_fold.
IPR022967. S1_dom.
[Graphical view]
PANTHERiPTHR11252. PTHR11252. 1 hit.
PfamiPF00013. KH_1. 1 hit.
PF03726. PNPase. 1 hit.
PF01138. RNase_PH. 2 hits.
PF03725. RNase_PH_C. 2 hits.
PF00575. S1. 1 hit.
[Graphical view]
PIRSFiPIRSF005499. PNPase. 1 hit.
SMARTiSM00322. KH. 1 hit.
SM00316. S1. 1 hit.
[Graphical view]
SUPFAMiSSF50249. SSF50249. 1 hit.
SSF54211. SSF54211. 2 hits.
SSF54791. SSF54791. 1 hit.
SSF55666. SSF55666. 2 hits.
TIGRFAMsiTIGR03591. polynuc_phos. 1 hit.
PROSITEiPS50084. KH_TYPE_1. 1 hit.
PS50126. S1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 43914 / DSM 3382 / HRM2.

Entry informationi

Entry nameiPNP_DESAH
AccessioniPrimary (citable) accession number: C0QHM6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 1, 2009
Last sequence update: May 5, 2009
Last modified: January 7, 2015
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.