C0QGT5 (LSPA_DESAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoprotein signal peptidase
EC=3.4.23.36

Alternative name(s):
Prolipoprotein signal peptidase
Signal peptidase II
Short name=SPase II

Gene names

Name:	lspA
Ordered Locus Names:	HRM2_24900

Organism

Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) [Complete proteome] [HAMAP]

Taxonomic identifier

177437 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfobacterales › Desulfobacteraceae › Desulfobacterium › Desulfobacterium autotrophicum

Protein attributes

Sequence length	162 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This protein specifically catalyzes the removal of signal peptides from prolipoproteins By similarity. HAMAP-Rule MF_00161
Catalytic activity	Release of signal peptides from bacterial membrane prolipoproteins. Hydrolyzes -Xaa-Yaa-Zaa-|-(S,diacylglyceryl)Cys-, in which Xaa is hydrophobic (preferably Leu), and Yaa (Ala or Ser) and Zaa (Gly or Ala) have small, neutral side chains. HAMAP-Rule MF_00161
Pathway	Protein modification; lipoprotein biosynthesis (signal peptide cleavage). HAMAP-Rule MF_00161
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity HAMAP-Rule MF_00161.
Sequence similarities	Belongs to the peptidase A8 family.

Ontologies

Keywords
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Aspartyl protease Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aspartic-type endopeptidase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 162	162	Lipoprotein signal peptidase HAMAP-Rule MF_00161		PRO_1000203592
Regions
	Transmembrane	9 – 29	21	Helical; Potential
	Transmembrane	39 – 59	21	Helical; Potential
	Transmembrane	66 – 86	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	136 – 156	21	Helical; Potential
Sites
	Active site	113	1	By similarity
	Active site	140	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0QGT5 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 32DB5EA008CA620E	FASTA	162	18,006
	10 20 30 40 50 60 MNTRALVRLC LVSTIIIALD QATKALVATT LVLHESIPVI HGFFNLTHIM NPGGAFGLFA 70 80 90 100 110 120 GHSPEVRKFF FLFVSSLVAL MILWLYQRTA QTHRVLSFGL AAIFAGAVGN LIDRFRFGMV 130 140 150 160 VDFLDFYLGA YHWPAFNVAD SAITIGMVVF VYHVIFNKVP DL

References

[1]

"Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide." Strittmatter A.W., Liesegang H., Rabus R., Decker I., Amann J., Andres S., Henne A., Fricke W.F., Martinez-Arias R., Bartels D., Goesmann A., Krause L., Puehler A., Klenk H.P., Richter M., Schuler M., Gloeckner F.O., Meyerdierks A., Gottschalk G., Amann R. Environ. Microbiol. 11:1038-1055(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43914 / DSM 3382 / HRM2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001087 Genomic DNA. Translation: ACN15584.1.
RefSeq	YP_002603748.1. NC_012108.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	177437.HRM2_24900.
Protein family/group databases
MEROPS	A08.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACN15584; ACN15584; HRM2_24900.
GeneID	7499993.
KEGG	dat:HRM2_24900.
PATRIC	21684681. VBIDesAut25181_2604.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0597.
HOGENOM	HOG000096992.
KO	K03101.
OMA	HFATFNV.
ProtClustDB	CLSK2402036.
Enzyme and pathway databases
BioCyc	DAUT177437:GHLR-2565-MONOMER.
UniPathway	UPA00665.
Family and domain databases
HAMAP	MF_00161. LspA.
InterPro	IPR001872. Peptidase_A8. [Graphical view]
Pfam	PF01252. Peptidase_A8. 1 hit. [Graphical view]
PRINTS	PR00781. LIPOSIGPTASE.
TIGRFAMs	TIGR00077. lspA. 1 hit.
PROSITE	PS00855. SPASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LSPA_DESAH

Accession

Primary (citable) accession number: C0QGT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program