C0QAX9 (FTHS_DESAH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate--tetrahydrofolate ligase
EC=6.3.4.3

Alternative name(s):
Formyltetrahydrofolate synthetase
Short name=FHS
Short name=FTHFS

Gene names

Name:	fhs
Ordered Locus Names:	HRM2_16700

Organism

Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) [Complete proteome] [HAMAP]

Taxonomic identifier

177437 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › delta/epsilon subdivisions › Deltaproteobacteria › Desulfobacterales › Desulfobacteraceae › Desulfobacterium › Desulfobacterium autotrophicum

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + formate + tetrahydrofolate = ADP + phosphate + 10-formyltetrahydrofolate. HAMAP-Rule MF_01543
Pathway	One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01543
Sequence similarities	Belongs to the formate--tetrahydrofolate ligase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	folic acid-containing compound biosynthetic process Inferred from electronic annotation. Source: InterPro tetrahydrofolate interconversion Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP formate-tetrahydrofolate ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 591	591	Formate--tetrahydrofolate ligase HAMAP-Rule MF_01543		PRO_1000215434
Regions
	Nucleotide binding	74 – 81	8	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0QAX9 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 136606B37A755835	FASTA	591	63,927
	10 20 30 40 50 60 MALDPTKHAD WEIAQDAEKD MLTIYEIGEK LGLTKEELLP QGHYIAKIDF RAVLARLKDK 70 80 90 100 110 120 PDGKYIDVTA ITPTPLGEGK STSSMGLVQG LGKLGKSVCA AIRQPSGGPT MNIKGSAAGG 130 140 150 160 170 180 GLAQCIPLTP FSLGFTGDIN AIMNAHNLAM VALTSRMQHE RNYTDEQLER LSGMKRIDID 190 200 210 220 230 240 PTRVEMGWIM DFCCQSLRNI IIGIDGVNGK SDGYMMKSKF GIAVSSEVMA ILAVAKDLKD 250 260 270 280 290 300 MRERMGKIVV AYTKKGKPVT TEDLQVAGAM TAWMVDALNP SLIQTLEGQP VLVHAGPFAN 310 320 330 340 350 360 IAIGQSSIIA DRVGLKLADY HVTESGFGAD IGFEKFWNLK CRFSGLKPDC AVIVATIRAL 370 380 390 400 410 420 KCHGGAPVPV PGKPMPEEYN TESVEWVEKG CANLLHHIRN VRKAGISPVV CINAFYSDTD 430 440 450 460 470 480 AEIAKVRELS EAEGARVALS RHWEKGGDGA IEFAETVIEA CEEETEFKFL YELDMPLKER 490 500 510 520 530 540 IELIAKEVYG ADGVDYSNEA NASLARIQAD PELAKLGMCM VKTHLSLSDN PSLKGVPTGW 550 560 570 580 590 RLMIREVLTY GGAGFIVPVA GTISLMPGTG SNPAFKRVDV DCETGKVEGV F

References

[1]

"Genome sequence of Desulfobacterium autotrophicum HRM2, a marine sulfate reducer oxidizing organic carbon completely to carbon dioxide." Strittmatter A.W., Liesegang H., Rabus R., Decker I., Amann J., Andres S., Henne A., Fricke W.F., Martinez-Arias R., Bartels D., Goesmann A., Krause L., Puehler A., Klenk H.P., Richter M., Schuler M., Gloeckner F.O., Meyerdierks A., Gottschalk G., Amann R. Environ. Microbiol. 11:1038-1055(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 43914 / DSM 3382 / HRM2.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP001087 Genomic DNA. Translation: ACN14778.1.
RefSeq	YP_002602940.1. NC_012108.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	177437.HRM2_16700.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACN14778; ACN14778; HRM2_16700.
GeneID	7499119.
KEGG	dat:HRM2_16700.
PATRIC	21683007. VBIDesAut25181_1780.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG2759.
HOGENOM	HOG000040280.
KO	K01938.
OMA	EIMAVLC.
ProtClustDB	PRK13507.
Enzyme and pathway databases
BioCyc	DAUT177437:GHLR-1746-MONOMER.
UniPathway	UPA00193.
Family and domain databases
HAMAP	MF_01543. FTHFS.
InterPro	IPR000559. Formate_THF_ligase. IPR020628. Formate_THF_ligase_CS. [Graphical view]
Pfam	PF01268. FTHFS. 1 hit. [Graphical view]
PROSITE	PS00721. FTHFS_1. 1 hit. PS00722. FTHFS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FTHS_DESAH

Accession

Primary (citable) accession number: C0QAX9

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 22, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program