C0Q872 (GHRA_SALPC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glyoxylate/hydroxypyruvate reductase A
EC=1.1.1.79
EC=1.1.1.81

Alternative name(s):
2-ketoacid reductase

Gene names

Name:	ghrA
Ordered Locus Names:	SPC_2615

Organism

Salmonella paratyphi C (strain RKS4594) [Complete proteome] [HAMAP]

Taxonomic identifier

476213 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Paratyphi C

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively By similarity. HAMAP-Rule MF_01666
Catalytic activity	Glycolate + NADP+ = glyoxylate + NADPH. HAMAP-Rule MF_01666 D-glycerate + NAD(P)+ = hydroxypyruvate + NAD(P)H. HAMAP-Rule MF_01666
Subcellular location	Cytoplasm By similarity HAMAP-Rule MF_01666.
Sequence similarities	Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	NAD NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from electronic annotation. Source: HAMAP
Molecular_function	cofactor binding Inferred from electronic annotation. Source: InterPro glyoxylate reductase (NADP) activity Inferred from electronic annotation. Source: HAMAP hydroxypyruvate reductase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 312	312	Glyoxylate/hydroxypyruvate reductase A HAMAP-Rule MF_01666		PRO_1000187279
Sites
	Active site	227	1	By similarity
	Active site	275	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0Q872 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: A46D708D25A7AD11	FASTA	312	35,034
	10 20 30 40 50 60 MEIIFYHPTF NAAWWVNALE KALPHARVRE WKVGDNNPAD YALVWQPPVE MLAGRRLKAV 70 80 90 100 110 120 FVLGAGVDAI LSKLNAHPEM LDASIPLFRL EDTGMGLQMQ EYAVSQVLHW FRRFDDYQAL 130 140 150 160 170 180 KNQALWKPLP EYTREEFSVG IMGAGVLGAK VAESLQAWGF PLRCWSRSRK SWPGVESYVG 190 200 210 220 230 240 REELRAFLNQ TRVLINLLPN TAQTVGIINS ELLDQLPDGA YVLNLARGVH VQEADLLAAL 250 260 270 280 290 300 DSGKLKGAML DVFSQEPLPQ ESPLWRHPRV AMTPHIAAVT RPAEAIDYIS RTITQLEKGE 310 PVTGQVDRAR GY

References

[1]

"Salmonella paratyphi C: genetic divergence from Salmonella choleraesuis and pathogenic convergence with Salmonella typhi." Liu W.-Q., Feng Y., Wang Y., Zou Q.-H., Chen F., Guo J.-T., Peng Y.-H., Jin Y., Li Y.-G., Hu S.-N., Johnston R.N., Liu G.-R., Liu S.-L. PLoS ONE 4:E4510-E4510(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RKS4594.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000857 Genomic DNA. Translation: ACN46717.1.
RefSeq	YP_002638158.1. NC_012125.1.
3D structure databases
ProteinModelPortal	C0Q872.
ModBase	Search...
Protein-protein interaction databases
STRING	476213.SPC_2615.
Proteomic databases
PRIDE	C0Q872.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACN46717; ACN46717; SPC_2615.
GeneID	7555137.
KEGG	sei:SPC_2615.
PATRIC	32364153. VBISalEnt12305_2687.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0111.
HOGENOM	HOG000136694.
KO	K12972.
OMA	HAVLRYL.
ProtClustDB	PRK15469.
Enzyme and pathway databases
BioCyc	SENT476213:GH8J-2655-MONOMER.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_01666. 2-Hacid_dh_C_GhrA.
InterPro	IPR006140. D-isomer_2_OHA_DH_NAD-bd. IPR023514. GhrA. IPR016040. NAD(P)-bd_dom. [Graphical view]
Pfam	PF02826. 2-Hacid_dh_C. 1 hit. [Graphical view]
PROSITE	PS00065. D_2_HYDROXYACID_DH_1. False negative. PS00670. D_2_HYDROXYACID_DH_2. False negative. PS00671. D_2_HYDROXYACID_DH_3. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GHRA_SALPC

Accession

Primary (citable) accession number: C0Q872

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 5, 2009
Last modified:	May 29, 2013
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program