C0PZV1 (RSMB_SALPC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase B
EC=2.1.1.176

Alternative name(s):
16S rRNA m5C967 methyltransferase
rRNA (cytosine-C(5)-)-methyltransferase RsmB

Gene names

Name:	rsmB
Synonyms:	sun
Ordered Locus Names:	SPC_3477

Organism

Salmonella paratyphi C (strain RKS4594) [Complete proteome] [HAMAP]

Taxonomic identifier

476213 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Salmonella › Salmonella choleraesuis › Salmonella enterica I › Salmonella enterica subsp. enterica serovar Paratyphi C

Protein attributes

Sequence length	429 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA By similarity. HAMAP-Rule MF_01856
Catalytic activity	S-adenosyl-L-methionine + cytosine(967) in 16S rRNA = S-adenosyl-L-homocysteine + 5-methylcytosine(967) in 16S rRNA. HAMAP-Rule MF_01856
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01856.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmB/NOP family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	RNA-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	RNA binding Inferred from electronic annotation. Source: UniProtKB-KW rRNA (cytosine) methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 429	429	Ribosomal RNA small subunit methyltransferase B HAMAP-Rule MF_01856		PRO_1000188697
Regions
	Region	254 – 260	7	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	375	1	Nucleophile Potential
	Binding site	277	1	S-adenosyl-L-methionine By similarity
	Binding site	303	1	S-adenosyl-L-methionine By similarity
	Binding site	322	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0PZV1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 7659006A4A90E8F7	FASTA	429	48,100
	10 20 30 40 50 60 MKKQNNLRSL AAQAVEQVVE QGQSLSNVLP PLQQKVADKD KALLQELCFG VLRTLSQLEW 70 80 90 100 110 120 LINKLMSRPM TGKQRTVHYL IMVGFYQLLY TRVPPHAALA ETVEGAVSIK RPQLKGLING 130 140 150 160 170 180 VLRQFQRQQE TLLNEFATSD ARFLHPGWLV KRLQNAYPTQ WQRIIDANNQ RPPMWLRVNR 190 200 210 220 230 240 THHTRDGWLG LLEDAGMKGY PHPDYPDAVR LETPAPVHAL PGFAEGWVTV QDASAQGCAV 250 260 270 280 290 300 FLAPQNGEHI LDLCAAPGGK TTHILEVAPE ADVLAVDIDE QRLSRVYDNL KRLGMKATVK 310 320 330 340 350 360 QGDGRYPAQW CGEQQFDRIL LDAPCSATGV IRRHPDIKWL RRDRDIAELA QLQAEILDAV 370 380 390 400 410 420 WPRLKPGGTL VYATCSVLPE ENRDQIKTFL QRTPDAALSE TGTPDQPGQQ NLPGGEEGDG FFYAKLIKK

References

[1]

"Salmonella paratyphi C: genetic divergence from Salmonella choleraesuis and pathogenic convergence with Salmonella typhi." Liu W.-Q., Feng Y., Wang Y., Zou Q.-H., Chen F., Guo J.-T., Peng Y.-H., Jin Y., Li Y.-G., Hu S.-N., Johnston R.N., Liu G.-R., Liu S.-L. PLoS ONE 4:E4510-E4510(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: RKS4594.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000857 Genomic DNA. Translation: ACN47561.1.
RefSeq	YP_002639002.1. NC_012125.1.
3D structure databases
ProteinModelPortal	C0PZV1.
SMR	C0PZV1. Positions 6-428.
ModBase	Search...
Protein-protein interaction databases
STRING	476213.SPC_3477.
Proteomic databases
PRIDE	C0PZV1.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	ACN47561; ACN47561; SPC_3477.
GeneID	7556022.
KEGG	sei:SPC_3477.
PATRIC	32365899. VBISalEnt12305_3533.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0144.
HOGENOM	HOG000037300.
KO	K03500.
OMA	KPPMWLR.
ProtClustDB	PRK10901.
Enzyme and pathway databases
BioCyc	SENT476213:GH8J-3539-MONOMER.
Family and domain databases
Gene3D	1.10.940.10. 1 hit.
HAMAP	MF_01856. 16SrRNA_methyltr_B.
InterPro	IPR001678. Fmu/NOL1/Nop2p. IPR018314. Fmu/NOL1/Nop2p_CS. IPR006027. NusB_RsmB_TIM44. IPR023267. RCMT. IPR004573. rRNA_ssu_MeTfrase_B. IPR023541. rRNA_ssu_MeTfrase_B_ent. [Graphical view]
Pfam	PF01189. Nol1_Nop2_Fmu. 1 hit. PF01029. NusB. 1 hit. [Graphical view]
PRINTS	PR02008. RCMTFAMILY.
SUPFAM	SSF48013. NusB_RsmB_TIM44. 1 hit.
TIGRFAMs	TIGR00563. rsmB. 1 hit.
PROSITE	PS01153. NOL1_NOP2_SUN. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RSMB_SALPC

Accession

Primary (citable) accession number: C0PZV1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 36 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program