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C0PN26 (LISC2_MAIZE) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 33. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Lipoyl synthase 2, chloroplastic

EC=2.8.1.8
Alternative name(s):
Lipoate synthase 2
Short name=LS 2
Short name=Lip-syn 2
Lipoate synthase, plastidial 2
Short name=LIP1p 2
Lipoic acid synthase 2
Gene names
Name:LIP1P-2
OrganismZea mays (Maize)
Taxonomic identifier4577 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea

Protein attributes

Sequence length367 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03129

Catalytic activity

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03129

Cofactor

Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.

Pathway

Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03129

Subcellular location

Plastidchloroplast HAMAP-Rule MF_03129.

Sequence similarities

Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
   Cellular componentChloroplast
Plastid
   DomainTransit peptide
   Ligand4Fe-4S
Iron
Iron-sulfur
Metal-binding
S-adenosyl-L-methionine
   Molecular functionTransferase
Gene Ontology (GO)
   Biological_processprotein lipoylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function4 iron, 4 sulfur cluster binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

lipoate synthase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 367Lipoyl synthase 2, chloroplastic HAMAP-Rule MF_03129PRO_0000398861

Sites

Metal binding841Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding891Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding951Iron-sulfur 1 (4Fe-4S) By similarity
Metal binding1211Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1251Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
Metal binding1281Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

Sequence LengthMass (Da)Tools
C0PN26 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 1D6C0E80C60C5A41

FASTA36739,767
        10         20         30         40         50         60 
MQSSLARPPV LAGCGERRGL AAVARCRAEA AAPVWTASRA SAGPYTGRDP EVKKPAWLRQ 

        70         80         90        100        110        120 
RAAQGEKYAR LRESIGELKL NTVCVEAQCP NIGECWNGGG GAGGEGDGIA TATIMVLGDT 

       130        140        150        160        170        180 
CTRGCRFCAV KTSNKPPPPD PLEPLNTALA VASWGVDYVV LTSVDRDDLP DGGSSHFAQT 

       190        200        210        220        230        240 
VKALKELKPG ILVECLTSDF RGDLEAISSL ANSGLDVYAH NIETVRSLQR VVRDPRAGYD 

       250        260        270        280        290        300 
QSLAVLKHAK GSREDMITKS SIMLGLGETD EEVKQAMMDL RAIGVDILTL GQYLQPSERH 

       310        320        330        340        350        360 
LTVREYVTPQ KFQFWKEYGE SVGFRYVASG PLVRSSYRAG ELFIQNLVRN NKTESSMDPY 


AEITKSN 

« Hide

References

[1]"Sequencing, mapping, and analysis of 27,455 maize full-length cDNAs."
Soderlund C., Descour A., Kudrna D., Bomhoff M., Boyd L., Currie J., Angelova A., Collura K., Wissotski M., Ashley E., Morrow D., Fernandes J., Walbot V., Yu Y.
PLoS Genet. 5:E1000740-E1000740(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: B73.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BT069695 mRNA. Translation: ACN36592.1.
RefSeqNP_001170321.1. NM_001176850.1.
UniGeneZm.155719.

3D structure databases

ProteinModelPortalC0PN26.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID100384288.
KEGGzma:100384288.

Organism-specific databases

GrameneC0PN26.

Phylogenomic databases

HOGENOMHOG000235998.
KOK03644.
OMAAHHLPVR.

Enzyme and pathway databases

UniPathwayUPA00538; UER00593.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERPTHR10949. PTHR10949. 1 hit.
PfamPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFPIRSF005963. Lipoyl_synth. 1 hit.
SMARTSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsTIGR00510. lipA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameLISC2_MAIZE
AccessionPrimary (citable) accession number: C0PN26
Entry history
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 5, 2009
Last modified: February 19, 2014
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways