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Protein

Lipoyl synthase 2, chloroplastic

Gene

LIP1P-2

Organism
Zea mays (Maize)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

Catalytic activityi

Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

[4Fe-4S] clusterUniRule annotationNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

Pathway:iprotein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, mitochondrial (LIP1), Lipoyl synthase, chloroplastic (LOC100283909), Lipoyl synthase 2, chloroplastic (LIP1P-2), Lipoyl synthase 1, chloroplastic (LIP1P-1)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi84 – 841Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi89 – 891Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S)UniRule annotation
Metal bindingi121 – 1211Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi125 – 1251Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
Metal bindingi128 – 1281Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase 2, chloroplastic (EC:2.8.1.8UniRule annotation)
Alternative name(s):
Lipoate synthase 2UniRule annotation
Short name:
LS 2UniRule annotation
Short name:
Lip-syn 2UniRule annotation
Lipoate synthase, plastidial 2UniRule annotation
Short name:
LIP1p 2UniRule annotation
Lipoic acid synthase 2UniRule annotation
Gene namesi
Name:LIP1P-2UniRule annotation
OrganismiZea mays (Maize)
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogoneaeZea
ProteomesiUP000007305 Componenti: Chromosome 3

Organism-specific databases

GrameneiC0PN26.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 367Lipoyl synthase 2, chloroplasticPRO_0000398861
Transit peptidei1 – ?ChloroplastUniRule annotation

Expressioni

Gene expression databases

ExpressionAtlasiC0PN26. baseline.

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G101698_P02.

Structurei

3D structure databases

ProteinModelPortaliC0PN26.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.
OMAiPRLYSIR.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0PN26-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQSSLARPPV LAGCGERRGL AAVARCRAEA AAPVWTASRA SAGPYTGRDP
60 70 80 90 100
EVKKPAWLRQ RAAQGEKYAR LRESIGELKL NTVCVEAQCP NIGECWNGGG
110 120 130 140 150
GAGGEGDGIA TATIMVLGDT CTRGCRFCAV KTSNKPPPPD PLEPLNTALA
160 170 180 190 200
VASWGVDYVV LTSVDRDDLP DGGSSHFAQT VKALKELKPG ILVECLTSDF
210 220 230 240 250
RGDLEAISSL ANSGLDVYAH NIETVRSLQR VVRDPRAGYD QSLAVLKHAK
260 270 280 290 300
GSREDMITKS SIMLGLGETD EEVKQAMMDL RAIGVDILTL GQYLQPSERH
310 320 330 340 350
LTVREYVTPQ KFQFWKEYGE SVGFRYVASG PLVRSSYRAG ELFIQNLVRN
360
NKTESSMDPY AEITKSN
Length:367
Mass (Da):39,767
Last modified:May 5, 2009 - v1
Checksum:i1D6C0E80C60C5A41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT069695 mRNA. Translation: ACN36592.1.
RefSeqiNP_001170321.1. NM_001176850.1.
UniGeneiZm.155719.

Genome annotation databases

EnsemblPlantsiGRMZM2G101698_T02; GRMZM2G101698_P02; GRMZM2G101698.
GeneIDi100384288.
KEGGizma:100384288.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT069695 mRNA. Translation: ACN36592.1.
RefSeqiNP_001170321.1. NM_001176850.1.
UniGeneiZm.155719.

3D structure databases

ProteinModelPortaliC0PN26.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi4577.GRMZM2G101698_P02.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiGRMZM2G101698_T02; GRMZM2G101698_P02; GRMZM2G101698.
GeneIDi100384288.
KEGGizma:100384288.

Organism-specific databases

GrameneiC0PN26.

Phylogenomic databases

HOGENOMiHOG000235998.
KOiK03644.
OMAiPRLYSIR.

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593.

Gene expression databases

ExpressionAtlasiC0PN26. baseline.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00206. Lipoyl_synth.
MF_03129. Lipoyl_synth_plantC.
InterProiIPR013785. Aldolase_TIM.
IPR006638. Elp3/MiaB/NifB.
IPR003698. Lipoyl_synth.
IPR027526. Lipoyl_synth_chlpt.
IPR007197. rSAM.
[Graphical view]
PANTHERiPTHR10949. PTHR10949. 1 hit.
PfamiPF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
SMARTiSM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00510. lipA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: B73.

Entry informationi

Entry nameiLISC2_MAIZE
AccessioniPrimary (citable) accession number: C0PN26
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: May 5, 2009
Last modified: July 22, 2015
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.