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Protein

Alpha-amylase

Gene

100383492

Organism
Zea mays (Maize)
Status
Unreviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units.UniRule annotation

Cofactori

Ca2+UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei208NucleophileUniRule annotation1
Active sitei234Proton donorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionGlycosidaseUniRule annotation, Hydrolase
Biological processCarbohydrate metabolismUniRule annotation

Protein family/group databases

CAZyiGH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
Alpha-amylaseUniRule annotation (EC:3.2.1.1UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan glucanohydrolaseUniRule annotation
Gene namesi
Name:100383492Imported
ORF Names:ZEAMMB73_Zm00001d031794Imported
OrganismiZea mays (Maize)Imported
Taxonomic identifieri4577 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaePACMAD cladePanicoideaeAndropogonodaeAndropogoneaeTripsacinaeZea
Proteomesi
  • UP000007305 Componenti: Chromosome 1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28UniRule annotationAdd BLAST28
ChainiPRO_501060494429 – 441Alpha-amylaseUniRule annotationAdd BLAST413

Interactioni

Protein-protein interaction databases

STRINGi4577.GRMZM2G070172_P01.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini29 – 375AamyInterPro annotationAdd BLAST347
Domaini370 – 430Alpha-amyl_C2InterPro annotationAdd BLAST61

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family.UniRule annotation

Keywords - Domaini

SignalUniRule annotation

Phylogenomic databases

eggNOGiKOG0471. Eukaryota.
COG0366. LUCA.
HOGENOMiHOG000239525.
KOiK01176.
OMAiVLNHKMG.
OrthoDBiEOG093600FS.

Family and domain databases

InterProiView protein in InterPro
IPR012850. A-amylase_bs_C.
IPR013775. A-amylase_pln.
IPR006046. Alpha_amylase.
IPR006047. Glyco_hydro_13_cat_dom.
IPR017853. Glycoside_hydrolase_SF.
PfamiView protein in Pfam
PF07821. Alpha-amyl_C2. 1 hit.
PF00128. Alpha-amylase. 1 hit.
PIRSFiPIRSF001028. Alph-amls_plant. 1 hit.
PRINTSiPR00110. ALPHAAMYLASE.
SMARTiView protein in SMART
SM00642. Aamy. 1 hit.
SM00810. Alpha-amyl_C2. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.

Sequencei

Sequence statusi: Complete.

C0PGE4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMKHSSSLCL LFLLALCTTL LACGLVQAQV LFQGFNWESC KQQGGWYNRL
60 70 80 90 100
KAQVDDIAKA GVTHVWLPPP SHSVSPQGYM PGRLYDLDAS KYGTAAELKS
110 120 130 140 150
LIAAFHGRGV QCVADIVINH RCAEKKDARG VYCIFEGGTP DDRLDWGPGM
160 170 180 190 200
ICSDDTQYSD GTGHRDTGEG FAAAPDIDHL NPRVQRELSA WLNWLRSDAV
210 220 230 240 250
GFDGWRLDFA KGYSPAVARM YVESTGPPSF VVAEIWNSLS YSGDGKPAPN
260 270 280 290 300
QDQCRQELLD WTRAVGGPAM AFDFPTKGLL QAGVQGELWR LRDSSGNAAG
310 320 330 340 350
LIGWAPEKAV TFVDNHDTGS TQKLWPFPSD KVMQGYAYIL THPGVPCIFY
360 370 380 390 400
DHMFDWNLKQ EISTLSAIRA RNGIRAGSKL RILVADADAY VAVVDEKVMV
410 420 430 440
KIGTRYGVSS VVPSDFHPAA HGKDYCVWEK ASLRVPAGRH L
Length:441
Mass (Da):48,416
Last modified:May 5, 2009 - v1
Checksum:i3AC31F7E00097F23
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT067363 mRNA. Translation: ACN34260.1.
CM007647 Genomic DNA. Translation: ONM03671.1.
RefSeqiNP_001169611.1. NM_001176140.1.
UniGeneiZm.38505.

Genome annotation databases

EnsemblPlantsiZm00001d031794_T001; Zm00001d031794_P001; Zm00001d031794.
GeneIDi100383492.
GrameneiZm00001d031794_T001; Zm00001d031794_P001; Zm00001d031794.
KEGGizma:100383492.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiC0PGE4_MAIZE
AccessioniPrimary (citable) accession number: C0PGE4
Entry historyiIntegrated into UniProtKB/TrEMBL: May 5, 2009
Last sequence update: May 5, 2009
Last modified: July 5, 2017
This is version 66 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported