C0M795 (NADE_STRE4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	SEQ_1792

Organism

Streptococcus equi subsp. equi (strain 4047) [Complete proteome] [HAMAP]

Taxonomic identifier

553482 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Streptococcaceae › Streptococcus

Protein attributes

Sequence length	274 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 274	274	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000191507
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	C0M795 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: CBC0EAD0C5A8461A	FASTA	274	30,380
	10 20 30 40 50 60 MTLQEDIIRQ LEVKAVIDPK QEIRQSVDFL KAYLLKHPFL KTYVLGISGG QDSSLAGKLA 70 80 90 100 110 120 QMAIEELRAE TGDEQYQFIA VRLPYGVQAD EADAQKALAF IQPDQALTVN IKEAVDGQLR 130 140 150 160 170 180 ALEAAGLEIS DFNKGNIKAR QRMISQYAIA GQTAGAVIGT DHAAENVTGF FTKFGDGGAD 190 200 210 220 230 240 ILPLFRLTKR QGKALLKVLK ADPSLYEKVP TADLEDKKPG LADEVALGVT YQEIDDYLEG 250 260 270 HTISAEAQAR IEDWWHKGQH KRHLPITIFD DFWK

References

[1]

"Genomic evidence for the evolution of Streptococcus equi: host restriction, increased virulence, and genetic exchange with human pathogens." Holden M.T.G., Heather Z., Paillot R., Steward K.F., Webb K., Ainslie F., Jourdan T., Bason N.C., Holroyd N.E., Mungall K., Quail M.A., Sanders M., Simmonds M., Willey D., Brooks K., Aanensen D.M., Spratt B.G., Jolley K.A. , Maiden M.C.J., Kehoe M., Chanter N., Bentley S.D., Robinson C., Maskell D.J., Parkhill J., Waller A.S. PLoS Pathog. 5:E1000346-E1000346(2009) [PubMed: 19325880] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 4047.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM204883 Genomic DNA. Translation: CAW94917.1.
RefSeq	YP_002747027.1. NC_012471.1.
3D structure databases
ProteinModelPortal	C0M795.
ModBase	Search...
Protein-protein interaction databases
STRING	C0M795.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	EBSTRT00000067746; EBSTRP00000062253; EBSTRG00000068511.
GeneID	7695716.
GenomeReviews	Gene locus SEQ_1792 in contig FM204883_GR.
KEGG	seu:SEQ_1792.
PATRIC	19648465. VBIStrEqu13040_1835.
Organism-specific databases
CMR	Search...
Phylogenomic databases
GeneTree	EBGT00050000027750.
OMA	VNIGPAV.
ProtClustDB	PRK00768.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_STRE4

Accession

Primary (citable) accession number: C0M795

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 28, 2009
Last sequence update:	May 5, 2009
Last modified:	January 25, 2012
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program