C0LGU0 (RLK_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable LRR receptor-like serine/threonine-protein kinase RLK
EC=2.7.11.1

Gene names

Name:	RLK
Ordered Locus Names:	At5g35390
ORF Names:	T26D22.9

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	662 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass type I membrane protein. Note: Preferentially localized to the apical region of the pollen tube plasma membrane. Ref.5
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 6 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence AAC13607.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence BAB11489.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 662	631	Probable LRR receptor-like serine/threonine-protein kinase RLK		PRO_0000387521
Regions
	Topological domain	32 – 256	225	Extracellular Potential
	Transmembrane	257 – 277	21	Helical; Potential
	Topological domain	278 – 662	385	Cytoplasmic Potential
	Repeat	79 – 98	20	LRR 1
	Repeat	99 – 121	23	LRR 2
	Repeat	122 – 144	23	LRR 3
	Repeat	147 – 169	23	LRR 4
	Repeat	171 – 191	21	LRR 5
	Repeat	192 – 214	23	LRR 6
	Domain	357 – 639	283	Protein kinase
	Nucleotide binding	363 – 371	9	ATP By similarity
Sites
	Binding site	385	1	ATP By similarity
Amino acid modifications
	Glycosylation	197	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	C0LGU0 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 1F441F0B84D9CE8C	FASTA	662	73,483
	10 20 30 40 50 60 MPPMQARTLS VYNVMVPLVC LLLFFSTPTH GLSDSEAILK FKESLVVGQE NALASWNAKS 70 80 90 100 110 120 PPCTWSGVLC NGGSVWRLQM ENLELSGSID IEALSGLTSL RTLSFMNNKF EGPFPDFKKL 130 140 150 160 170 180 AALKSLYLSN NQFGGDIPGD AFEGMGWLKK VHLAQNKFTG QIPSSVAKLP KLLELRLDGN 190 200 210 220 230 240 QFTGEIPEFE HQLHLLNLSN NALTGPIPES LSMTDPKVFE GNKGLYGKPL ETECDSPYIE 250 260 270 280 290 300 HPPQSEARPK SSSRGPLVIT AIVAALTILI ILGVIFLLNR SYKNKKPRLA VETGPSSLQK 310 320 330 340 350 360 KTGIREADQS RRDRKKADHR KGSGTTKRMG AAAGVENTKL SFLREDREKF DLQDLLKASA 370 380 390 400 410 420 EILGSGCFGA SYKAVLSSGQ MMVVKRFKQM NNAGRDEFQE HMKRLGRLMH HNLLSIVAYY 430 440 450 460 470 480 YRKEEKLLVC DFAERGSLAI NLHSNQSLGK PSLDWPTRLK IVKGVAKGLF YLHQDLPSLM 490 500 510 520 530 540 APHGHLKSSN VLLTKTFEPL LTDYGLIPLI NQEKAQMHMA AYRSPEYLQH RRITKKTDVW 550 560 570 580 590 600 GLGILILEIL TGKFPANFSQ SSEEDLASWV NSGFHGVWAP SLFDKGMGKT SHCEGQILKL 610 620 630 640 650 660 LTIGLNCCEP DVEKRLDIGQ AVEKIEELKE REGDDDDFYS TYVSETDGRS SKGESCESIS FA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana." Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K. , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F. Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Structural analysis of Arabidopsis thaliana chromosome 5. XI." Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth." Lee Y.J., Szumlanski A., Nielsen E., Yang Z. J. Cell Biol. 181:1155-1168(2008) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF058826 Genomic DNA. Translation: AAC13607.1. Sequence problems. AB025636 Genomic DNA. Translation: BAB11489.1. Sequence problems. CP002688 Genomic DNA. Translation: AED93962.1. FJ708784 mRNA. Translation: ACN59375.1.
IPI	IPI00539094.
PIR	T01183.
RefSeq	NP_198389.2. NM_122930.2.
UniGene	At.55132.
3D structure databases
ProteinModelPortal	C0LGU0.
SMR	C0LGU0. Positions 34-263, 348-626.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT5G35390.1-P.
Proteomic databases
PaxDb	C0LGU0.
PRIDE	C0LGU0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT5G35390.1; AT5G35390.1; AT5G35390.
GeneID	833500.
KEGG	ath:AT5G35390.
Organism-specific databases
GeneFarm	550. 47.
TAIR	At5g35390.
Phylogenomic databases
eggNOG	KOG0619.
HOGENOM	HOG000116554.
InParanoid	O65240.
OMA	TDYGLIP.
PhylomeDB	C0LGU0.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. PF08263. LRRNT_2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RLK_ARATH

Accession

Primary (citable) accession number: C0LGU0
Secondary accession number(s): O65240

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program