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Protein

Pollen receptor-like kinase 1

Gene

PRK1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Receptor-like kinase involved in the control of pollen germination and pollen tube polar growth (PubMed:23024212).1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.Curated

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei385 – 3851ATPPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi363 – 3719ATPPROSITE-ProRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT5G35390-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Pollen receptor-like kinase 11 Publication (EC:2.7.11.1Curated)
Short name:
AtPRK11 Publication
Alternative name(s):
LRR receptor-like serine/threonine-protein kinase RLK
Gene namesi
Name:PRK11 Publication
Synonyms:PRKD1 Publication, RLK
Ordered Locus Names:At5g35390Imported
ORF Names:T26D22.9Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G35390.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini32 – 256225ExtracellularSequence AnalysisAdd
BLAST
Transmembranei257 – 27721HelicalSequence AnalysisAdd
BLAST
Topological domaini278 – 662385CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No effect on pollen germination and growth. Prk1 and prk2 double mutant has no effect on pollen germination and growth. Prk1, prk2 and prk5 triple mutant shows reduced pollen tube elongation.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 3131Sequence AnalysisAdd
BLAST
Chaini32 – 662631Pollen receptor-like kinase 1PRO_0000387521Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi197 – 1971N-linked (GlcNAc...)Sequence Analysis
Modified residuei359 – 3591PhosphoserineBy similarity
Modified residuei437 – 4371PhosphoserineBy similarity
Modified residuei457 – 4571PhosphothreonineBy similarity
Modified residuei527 – 5271PhosphotyrosineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiC0LGU0.
PRIDEiC0LGU0.

Expressioni

Tissue specificityi

Expressed in pollen and/or in flowers, but not in leaves.1 Publication

Interactioni

Subunit structurei

Interacts in vitro with ROPGEF1 (via PRONE domain).1 Publication

Protein-protein interaction databases

STRINGi3702.AT5G35390.1.

Structurei

3D structure databases

ProteinModelPortaliC0LGU0.
SMRiC0LGU0. Positions 33-242, 322-638.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati79 – 9820LRR 1Add
BLAST
Repeati99 – 12123LRR 2Add
BLAST
Repeati122 – 14423LRR 3Add
BLAST
Repeati147 – 16923LRR 4Add
BLAST
Repeati171 – 19121LRR 5Add
BLAST
Repeati192 – 21423LRR 6Add
BLAST
Domaini357 – 639283Protein kinasePROSITE-ProRule annotationAdd
BLAST

Domaini

The protein kinase domain may be catalytically impaired due to the lack of the conserved Asp active site at position 485, which is replaced by a His residue.PROSITE-ProRule annotation

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 6 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0619.
HOGENOMiHOG000116554.
InParanoidiC0LGU0.
OMAiKNSEGEM.
PhylomeDBiC0LGU0.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0LGU0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPMQARTLS VYNVMVPLVC LLLFFSTPTH GLSDSEAILK FKESLVVGQE
60 70 80 90 100
NALASWNAKS PPCTWSGVLC NGGSVWRLQM ENLELSGSID IEALSGLTSL
110 120 130 140 150
RTLSFMNNKF EGPFPDFKKL AALKSLYLSN NQFGGDIPGD AFEGMGWLKK
160 170 180 190 200
VHLAQNKFTG QIPSSVAKLP KLLELRLDGN QFTGEIPEFE HQLHLLNLSN
210 220 230 240 250
NALTGPIPES LSMTDPKVFE GNKGLYGKPL ETECDSPYIE HPPQSEARPK
260 270 280 290 300
SSSRGPLVIT AIVAALTILI ILGVIFLLNR SYKNKKPRLA VETGPSSLQK
310 320 330 340 350
KTGIREADQS RRDRKKADHR KGSGTTKRMG AAAGVENTKL SFLREDREKF
360 370 380 390 400
DLQDLLKASA EILGSGCFGA SYKAVLSSGQ MMVVKRFKQM NNAGRDEFQE
410 420 430 440 450
HMKRLGRLMH HNLLSIVAYY YRKEEKLLVC DFAERGSLAI NLHSNQSLGK
460 470 480 490 500
PSLDWPTRLK IVKGVAKGLF YLHQDLPSLM APHGHLKSSN VLLTKTFEPL
510 520 530 540 550
LTDYGLIPLI NQEKAQMHMA AYRSPEYLQH RRITKKTDVW GLGILILEIL
560 570 580 590 600
TGKFPANFSQ SSEEDLASWV NSGFHGVWAP SLFDKGMGKT SHCEGQILKL
610 620 630 640 650
LTIGLNCCEP DVEKRLDIGQ AVEKIEELKE REGDDDDFYS TYVSETDGRS
660
SKGESCESIS FA
Length:662
Mass (Da):73,483
Last modified:May 5, 2009 - v1
Checksum:i1F441F0B84D9CE8C
GO

Sequence cautioni

The sequence AAC13607.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence BAB11489.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058826 Genomic DNA. Translation: AAC13607.1. Sequence problems.
AB025636 Genomic DNA. Translation: BAB11489.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93962.1.
FJ708784 mRNA. Translation: ACN59375.1.
PIRiT01183.
RefSeqiNP_198389.2. NM_122930.2.
UniGeneiAt.55132.

Genome annotation databases

EnsemblPlantsiAT5G35390.1; AT5G35390.1; AT5G35390.
GeneIDi833500.
KEGGiath:AT5G35390.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058826 Genomic DNA. Translation: AAC13607.1. Sequence problems.
AB025636 Genomic DNA. Translation: BAB11489.1. Sequence problems.
CP002688 Genomic DNA. Translation: AED93962.1.
FJ708784 mRNA. Translation: ACN59375.1.
PIRiT01183.
RefSeqiNP_198389.2. NM_122930.2.
UniGeneiAt.55132.

3D structure databases

ProteinModelPortaliC0LGU0.
SMRiC0LGU0. Positions 33-242, 322-638.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT5G35390.1.

Proteomic databases

PaxDbiC0LGU0.
PRIDEiC0LGU0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G35390.1; AT5G35390.1; AT5G35390.
GeneIDi833500.
KEGGiath:AT5G35390.

Organism-specific databases

GeneFarmi550. 47.
TAIRiAT5G35390.

Phylogenomic databases

eggNOGiKOG0619.
HOGENOMiHOG000116554.
InParanoidiC0LGU0.
OMAiKNSEGEM.
PhylomeDBiC0LGU0.

Enzyme and pathway databases

BioCyciARA:AT5G35390-MONOMER.

Miscellaneous databases

PROiC0LGU0.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana."
    Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E., Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.
    , Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L., O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D., Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M., Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L., Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B., Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P., Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M., Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D., Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A., Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I., Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T., Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A., McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U., Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M., Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G., Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W., Bevan M., Fransz P.F.
    Nature 408:823-826(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. XI."
    Kaneko T., Katoh T., Asamizu E., Sato S., Nakamura Y., Kotani H., Tabata S.
    Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
    Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
    BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  5. "New pollen-specific receptor kinases identified in tomato, maize and Arabidopsis: the tomato kinases show overlapping but distinct localization patterns on pollen tubes."
    Kim H.U., Cotter R., Johnson S., Senda M., Dodds P., Kulikauska R., Tang W., Ezcura I., Herzmark P., McCormick S.
    Plant Mol. Biol. 50:1-16(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, GENE FAMILY, NOMENCLATURE.
  6. "Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth."
    Lee Y.J., Szumlanski A., Nielsen E., Yang Z.
    J. Cell Biol. 181:1155-1168(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  7. "AtPRK2 Promotes ROP1 activation via RopGEFs in the control of polarized pollen tube growth."
    Chang F., Gu Y., Ma H., Yang Z.
    Mol. Plant 6:1187-1201(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DISRUPTION PHENOTYPE, GENE FAMILY, NOMENCLATURE, INTERACTION WITH ROPGEF1.

Entry informationi

Entry nameiPRK1_ARATH
AccessioniPrimary (citable) accession number: C0LGU0
Secondary accession number(s): O65240
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: July 22, 2015
This is version 51 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.