Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

LRR receptor-like serine/threonine-protein kinase EFR

Gene

EFR

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Constitutes the pattern-recognition receptor (PPR) that determines the specific perception of elongation factor Tu (EF-Tu), a potent elicitor of the defense response to pathogen-associated molecular patterns (PAMPs). Reduces transformation by Rhizobium radiobacter probably by inducing plant defense during the interaction. Binding to the effector AvrPto1 from P.syringae blocks the downstream plant immune response while interaction with hopD2 decreases the phosphorylation level of EFR upon elf18 treatment. Specific endoplasmic reticulum quality control components (ERD2B, CRT3, UGGT and STT3A) are required for the biogenesis of EFR.6 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei741ATPPROSITE-ProRule annotation1
Active sitei849Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi718 – 726ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

  • defense response signaling pathway, resistance gene-independent Source: TAIR
  • detection of bacterium Source: TAIR
  • immune response-regulating signaling pathway Source: TAIR
  • plant-type hypersensitive response Source: TAIR
  • regulation of anion channel activity Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Plant defense

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase EFR (EC:2.7.11.1)
Alternative name(s):
Elongation factor Tu receptor
Short name:
EF-Tu receptor
Gene namesi
Name:EFR
Ordered Locus Names:At5g20480
ORF Names:F7C8.70
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

TAIRiAT5G20480.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 653ExtracellularSequence analysisAdd BLAST629
Transmembranei654 – 674HelicalSequence analysisAdd BLAST21
Topological domaini675 – 1031CytoplasmicSequence analysisAdd BLAST357

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Enhanced susceptibility to R.radiobacter.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi76 – 77IS → AA: No effect on elf18 sensitivity. 1 Publication2
Mutagenesisi81 – 82GG → AA: No effect on elf18 sensitivity. 1 Publication2
Mutagenesisi103 – 106NLAD → ALAA: Insensitive to elf18. 1 Publication4
Mutagenesisi274 – 275GT → AA: No effect on elf18 sensitivity. 1 Publication2
Mutagenesisi293 – 294ER → AA: No effect on elf18 sensitivity. 1 Publication2
Mutagenesisi298 – 299SS → AA: No effect on elf18 sensitivity. 1 Publication2
Mutagenesisi317 – 318WW → AA: Decreased elf18 sensitivity. 1 Publication2
Mutagenesisi347 – 348EY → AA: Decreased elf18 sensitivity. 1 Publication2
Mutagenesisi373 – 375SLF → ALA: No effect on elf18 sensitivity. 1 Publication3
Mutagenesisi397 – 399ELS → ALA: Decreased elf18 sensitivity. 1 Publication3
Mutagenesisi447 – 449HLN → ALA: Insensitive to elf18. 1 Publication3
Mutagenesisi469 – 473DLWMD → ALWMA: Insensitive to elf18. 1 Publication5
Mutagenesisi564 – 568NVDFS → AVDFA: Decreased elf18 sensitivity. 1 Publication5
Mutagenesisi588 – 590NLN → ALA: Insensitive to elf18. 1 Publication3
Mutagenesisi702Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi791Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi836Y → F: Loss of elf18-triggered immunity, but no effect on the kinase activity. 1 Publication1
Mutagenesisi849D → N: Loss of kinase activity. 1 Publication1
Mutagenesisi875Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi877Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi897Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi902Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi915Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi939Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi944Y → F: No effect on elf18-triggered immunity. 1 Publication1
Mutagenesisi979Y → F: No effect on elf18-triggered immunity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000038750825 – 1031LRR receptor-like serine/threonine-protein kinase EFRAdd BLAST1007

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi28N-linked (GlcNAc...)Sequence analysis1
Glycosylationi55N-linked (GlcNAc...)Sequence analysis1
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1
Glycosylationi127N-linked (GlcNAc...)Sequence analysis1
Glycosylationi143N-linked (GlcNAc...)Sequence analysis1
Glycosylationi180N-linked (GlcNAc...)Sequence analysis1
Glycosylationi191N-linked (GlcNAc...)Sequence analysis1
Glycosylationi239N-linked (GlcNAc...)Sequence analysis1
Glycosylationi288N-linked (GlcNAc...)Sequence analysis1
Glycosylationi323N-linked (GlcNAc...)Sequence analysis1
Glycosylationi329N-linked (GlcNAc...)Sequence analysis1
Glycosylationi342N-linked (GlcNAc...)Sequence analysis1
Glycosylationi366N-linked (GlcNAc...)Sequence analysis1
Glycosylationi439N-linked (GlcNAc...)Sequence analysis1
Glycosylationi478N-linked (GlcNAc...)Sequence analysis1
Glycosylationi571N-linked (GlcNAc...)Sequence analysis1
Glycosylationi590N-linked (GlcNAc...)Sequence analysis1
Glycosylationi608N-linked (GlcNAc...)Sequence analysis1
Modified residuei709PhosphothreonineBy similarity1
Modified residuei791PhosphotyrosineBy similarity1
Modified residuei836Phosphotyrosine1 Publication1
Modified residuei897Phosphotyrosine1 Publication1

Post-translational modificationi

Autophosphorylated after elicitation with elfl18. Autophosphorylation is inhibited by the binding with avrPto1. Phosphorylation at T-836 is required for immune signaling.1 Publication
Polyubiquitinated at the kinase domain mediated by P.syringae AvrPtoB.1 Publication

Keywords - PTMi

Glycoprotein, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiC0LGT6.

PTM databases

iPTMnetiC0LGT6.

Expressioni

Gene expression databases

GenevisibleiC0LGT6. AT.

Interactioni

Subunit structurei

Binds to Pseudomonas syringae AvrPto1 and (via the kinase and cytoplasmic domains) to hopD2. Interacts with SERK3/BAK1, SERK4/BKK1, SERK1 and SERK2 in a specific ligand-induced manner.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
RBG7Q032504EBI-8801168,EBI-1393626

Protein-protein interaction databases

BioGridi17446. 4 interactors.
DIPiDIP-61674N.
IntActiC0LGT6. 1 interactor.
STRINGi3702.AT5G20480.1.

Structurei

3D structure databases

ProteinModelPortaliC0LGT6.
SMRiC0LGT6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati98 – 120LRR 1Add BLAST23
Repeati122 – 144LRR 2Add BLAST23
Repeati146 – 168LRR 3Add BLAST23
Repeati170 – 193LRR 4Add BLAST24
Repeati194 – 216LRR 5Add BLAST23
Repeati218 – 240LRR 6Add BLAST23
Repeati242 – 264LRR 7Add BLAST23
Repeati267 – 289LRR 8Add BLAST23
Repeati291 – 312LRR 9Add BLAST22
Repeati315 – 335LRR 10Add BLAST21
Repeati345 – 368LRR 11Add BLAST24
Repeati370 – 392LRR 12Add BLAST23
Repeati394 – 416LRR 13Add BLAST23
Repeati418 – 440LRR 14Add BLAST23
Repeati442 – 464LRR 15Add BLAST23
Repeati466 – 487LRR 16Add BLAST22
Repeati490 – 512LRR 17Add BLAST23
Repeati514 – 536LRR 18Add BLAST23
Repeati538 – 560LRR 19Add BLAST23
Repeati561 – 584LRR 20Add BLAST24
Repeati585 – 597LRR 21Add BLAST13
Domaini712 – 1001Protein kinasePROSITE-ProRule annotationAdd BLAST290

Domaini

The last two LRR (561-597) are necessary for elf18 binding and functionality.1 Publication

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 21 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IH5R. Eukaryota.
COG0515. LUCA.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiC0LGT6.
KOiK13428.
OMAiSGRIPRY.
OrthoDBiEOG093601N6.
PhylomeDBiC0LGT6.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 8 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0LGT6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLSFSLVFN ALTLLLQVCI FAQARFSNET DMQALLEFKS QVSENNKREV
60 70 80 90 100
LASWNHSSPF CNWIGVTCGR RRERVISLNL GGFKLTGVIS PSIGNLSFLR
110 120 130 140 150
LLNLADNSFG STIPQKVGRL FRLQYLNMSY NLLEGRIPSS LSNCSRLSTV
160 170 180 190 200
DLSSNHLGHG VPSELGSLSK LAILDLSKNN LTGNFPASLG NLTSLQKLDF
210 220 230 240 250
AYNQMRGEIP DEVARLTQMV FFQIALNSFS GGFPPALYNI SSLESLSLAD
260 270 280 290 300
NSFSGNLRAD FGYLLPNLRR LLLGTNQFTG AIPKTLANIS SLERFDISSN
310 320 330 340 350
YLSGSIPLSF GKLRNLWWLG IRNNSLGNNS SSGLEFIGAV ANCTQLEYLD
360 370 380 390 400
VGYNRLGGEL PASIANLSTT LTSLFLGQNL ISGTIPHDIG NLVSLQELSL
410 420 430 440 450
ETNMLSGELP VSFGKLLNLQ VVDLYSNAIS GEIPSYFGNM TRLQKLHLNS
460 470 480 490 500
NSFHGRIPQS LGRCRYLLDL WMDTNRLNGT IPQEILQIPS LAYIDLSNNF
510 520 530 540 550
LTGHFPEEVG KLELLVGLGA SYNKLSGKMP QAIGGCLSME FLFMQGNSFD
560 570 580 590 600
GAIPDISRLV SLKNVDFSNN NLSGRIPRYL ASLPSLRNLN LSMNKFEGRV
610 620 630 640 650
PTTGVFRNAT AVSVFGNTNI CGGVREMQLK PCIVQASPRK RKPLSVRKKV
660 670 680 690 700
VSGICIGIAS LLLIIIVASL CWFMKRKKKN NASDGNPSDS TTLGMFHEKV
710 720 730 740 750
SYEELHSATS RFSSTNLIGS GNFGNVFKGL LGPENKLVAV KVLNLLKHGA
760 770 780 790 800
TKSFMAECET FKGIRHRNLV KLITVCSSLD SEGNDFRALV YEFMPKGSLD
810 820 830 840 850
MWLQLEDLER VNDHSRSLTP AEKLNIAIDV ASALEYLHVH CHDPVAHCDI
860 870 880 890 900
KPSNILLDDD LTAHVSDFGL AQLLYKYDRE SFLNQFSSAG VRGTIGYAAP
910 920 930 940 950
EYGMGGQPSI QGDVYSFGIL LLEMFSGKKP TDESFAGDYN LHSYTKSILS
960 970 980 990 1000
GCTSSGGSNA IDEGLRLVLQ VGIKCSEEYP RDRMRTDEAV RELISIRSKF
1010 1020 1030
FSSKTTITES PRDAPQSSPQ EWMLNTDMHT M
Length:1,031
Mass (Da):113,353
Last modified:May 5, 2009 - v1
Checksum:iE0E0AE671DA9124D
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti893G → L in BAE98400 (Ref. 5) Curated1
Sequence conflicti929K → E in AAL77697 (PubMed:14593172).Curated1
Sequence conflicti929K → E in AAO64755 (PubMed:14593172).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296833 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92850.1.
AY075690 mRNA. Translation: AAL77697.1.
BT005820 mRNA. Translation: AAO64755.1.
FJ708780 mRNA. Translation: ACN59371.1.
AK226237 mRNA. Translation: BAE98400.1.
RefSeqiNP_197548.1. NM_122055.5.
UniGeneiAt.31176.

Genome annotation databases

EnsemblPlantsiAT5G20480.1; AT5G20480.1; AT5G20480.
GeneIDi832170.
GrameneiAT5G20480.1; AT5G20480.1; AT5G20480.
KEGGiath:AT5G20480.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF296833 Genomic DNA. No translation available.
CP002688 Genomic DNA. Translation: AED92850.1.
AY075690 mRNA. Translation: AAL77697.1.
BT005820 mRNA. Translation: AAO64755.1.
FJ708780 mRNA. Translation: ACN59371.1.
AK226237 mRNA. Translation: BAE98400.1.
RefSeqiNP_197548.1. NM_122055.5.
UniGeneiAt.31176.

3D structure databases

ProteinModelPortaliC0LGT6.
SMRiC0LGT6.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi17446. 4 interactors.
DIPiDIP-61674N.
IntActiC0LGT6. 1 interactor.
STRINGi3702.AT5G20480.1.

PTM databases

iPTMnetiC0LGT6.

Proteomic databases

PaxDbiC0LGT6.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT5G20480.1; AT5G20480.1; AT5G20480.
GeneIDi832170.
GrameneiAT5G20480.1; AT5G20480.1; AT5G20480.
KEGGiath:AT5G20480.

Organism-specific databases

TAIRiAT5G20480.

Phylogenomic databases

eggNOGiENOG410IH5R. Eukaryota.
COG0515. LUCA.
COG4886. LUCA.
HOGENOMiHOG000116551.
InParanoidiC0LGT6.
KOiK13428.
OMAiSGRIPRY.
OrthoDBiEOG093601N6.
PhylomeDBiC0LGT6.

Miscellaneous databases

PROiC0LGT6.

Gene expression databases

GenevisibleiC0LGT6. AT.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 3 hits.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR003591. Leu-rich_rpt_typical-subtyp.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00369. LRR_TYP. 8 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 2 hits.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEFR_ARATH
AccessioniPrimary (citable) accession number: C0LGT6
Secondary accession number(s): Q0WWU8, Q8S9I3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.