C0LGS3 (Y4372_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable LRR receptor-like serine/threonine-protein kinase At4g37250
EC=2.7.11.1

Gene names

Ordered Locus Names:	At4g37250
ORF Names:	AP22.22, C7A10.110

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	768 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 8 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence CAB16774.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB80391.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Membrane
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21	Potential
	Chain	22 – 768	747	Probable LRR receptor-like serine/threonine-protein kinase At4g37250		PRO_0000387558
Regions
	Topological domain	22 – 328	307	Extracellular Potential
	Transmembrane	329 – 349	21	Helical; Potential
	Topological domain	350 – 768	419	Cytoplasmic Potential
	Repeat	67 – 90	24	LRR 1
	Repeat	91 – 112	22	LRR 2
	Repeat	115 – 137	23	LRR 3
	Repeat	139 – 162	24	LRR 4
	Repeat	163 – 183	21	LRR 5
	Repeat	184 – 206	23	LRR 6
	Repeat	207 – 229	23	LRR 7
	Repeat	232 – 254	23	LRR 8
	Domain	449 – 756	308	Protein kinase
Amino acid modifications
	Glycosylation	64	1	N-linked (GlcNAc...) Potential
	Glycosylation	99	1	N-linked (GlcNAc...) Potential
	Glycosylation	144	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	196	1	N-linked (GlcNAc...) Potential
	Glycosylation	212	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Glycosylation	242	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	361	1	D → Y in AAN72248. Ref.4
	Sequence conflict	361	1	D → Y in AAL57701. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	C0LGS3 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 03057CD65F6A364B	FASTA	768	84,063
	10 20 30 40 50 60 MRMELISVIF FFFCSVLSSS ALNSDGLVLM KFKSSVLVDP LSLLQTWNYK HESPCSWRGI 70 80 90 100 110 120 SCNNDSKVLT LSLPNSQLLG SIPSDLGSLL TLQSLDLSNN SFNGPLPVSF FNARELRFLD 130 140 150 160 170 180 LSSNMISGEI PSAIGDLHNL LTLNLSDNAL AGKLPTNLAS LRNLTVVSLE NNYFSGEIPG 190 200 210 220 230 240 GWRVVEFLDL SSNLINGSLP PDFGGYSLQY LNVSFNQISG EIPPEIGVNF PRNVTVDLSF 250 260 270 280 290 300 NNLTGPIPDS PVFLNQESNF FSGNPGLCGE PTRNPCLIPS SPSIVSEADV PTSTPAIAAI 310 320 330 340 350 360 PNTIGSNPVT DPNSQQTDPN PRTGLRPGVI IGIVVGDIAG IGILAVIFLY IYRCKKNKIV 370 380 390 400 410 420 DNNNNDKQRT ETDTITLSTF SSSSSSPEES RRFRKWSCLR KDPETTPSEE EDEDDEDEES 430 440 450 460 470 480 GYNANQRSGD NKLVTVDGEK EMEIETLLKA SAYILGATGS SIMYKAVLED GRVFAVRRLG 490 500 510 520 530 540 ENGLSQRRFK DFEPHIRAIG KLVHPNLVRL CGFYWGTDEK LVIYDFVPNG SLVNPRYRKG 550 560 570 580 590 600 GGSSSPYHLP WETRLKIAKG IARGLAYLHE KKHVHGNLKP SNILLGHDME PKIGDFGLER 610 620 630 640 650 660 LLTGETSYIR AGGSSRIFSS KRYTTSSREF SSIGPTPSPS PSSVGAMSPY CAPESFRSLK 670 680 690 700 710 720 PSPKWDVYGF GVILLELLTG KIVSVEEIVL GNGLTVEDGH RAVRMADVAI RGELDGKQEF 730 740 750 760 LLDCFKLGYS CASPVPQKRP TMKESLAVLE RFHPNSSVIK SSSFHYGH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of 1.9 Mb of contiguous sequence from chromosome 4 of Arabidopsis thaliana." Bevan M., Bancroft I., Bent E., Love K., Goodman H.M., Dean C., Bergkamp R., Dirkse W., van Staveren M., Stiekema W., Drost L., Ridley P., Hudson S.-A., Patel K., Murphy G., Piffanelli P., Wedler H., Wedler E. , Wambutt R., Weitzenegger T., Pohl T., Terryn N., Gielen J., Villarroel R., De Clercq R., van Montagu M., Lecharny A., Aubourg S., Gy I., Kreis M., Lao N., Kavanagh T., Hempel S., Kotter P., Entian K.-D., Rieger M., Schaefer M., Funk B., Mueller-Auer S., Silvey M., James R., Monfort A., Pons A., Puigdomenech P., Douka A., Voukelatou E., Milioni D., Hatzopoulos P., Piravandi E., Obermaier B., Hilbert H., Duesterhoeft A., Moores T., Jones J.D.G., Eneva T., Palme K., Benes V., Rechmann S., Ansorge W., Cooke R., Berger C., Delseny M., Voet M., Volckaert G., Mewes H.-W., Klosterman S., Schueller C., Chalwatzis N. Nature 391:485-488(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[6]	"Analysis of multiple occurrences of alternative splicing events in Arabidopsis thaliana using novel sequenced full-length cDNAs." Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M., Shinozaki K. DNA Res. 16:155-164(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 71-768. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z99707 Genomic DNA. Translation: CAB16774.1. Different initiation. AL161591 Genomic DNA. Translation: CAB80391.1. Different initiation. CP002687 Genomic DNA. Translation: AEE86773.1. AY065068 mRNA. Translation: AAL57701.1. BT002237 mRNA. Translation: AAN72248.1. FJ708765 mRNA. Translation: ACN59358.1. AK317633 mRNA. Translation: BAH20295.1.
IPI	IPI00530748.
PIR	B85440.
RefSeq	NP_195442.2. NM_119888.2.
UniGene	At.27846.
3D structure databases
ProteinModelPortal	C0LGS3.
SMR	C0LGS3. Positions 29-263, 440-750.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G37250.1; AT4G37250.1; AT4G37250.
GeneID	829879.
KEGG	ath:AT4G37250.
Organism-specific databases
GeneFarm	596. 47.
TAIR	At4g37250.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000116554.
InParanoid	Q8VZC5.
OMA	YSEVELC.
PhylomeDB	C0LGS3.
ProtClustDB	CLSN2683884.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF00560. LRR_1. 2 hits. PF08263. LRRNT_2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y4372_ARATH

Accession

Primary (citable) accession number: C0LGS3
Secondary accession number(s): B9DHS8, O23161, Q8VZC5

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program