C0LGR9 (Y4312_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable LRR receptor-like serine/threonine-protein kinase At4g31250
EC=2.7.11.1

Gene names

Ordered Locus Names:	At4g31250
ORF Names:	F8F16.70

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	676 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Membrane; Single-pass type I membrane protein Potential.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 5 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence CAA16528.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79843.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: C0LGR9-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: C0LGR9-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-88: Missing.
     89-98: ELDVQALGSI → MERSDVFKRL

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 676	650	Probable LRR receptor-like serine/threonine-protein kinase At4g31250		PRO_0000387556
Regions
	Topological domain	27 – 242	216	Extracellular Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Topological domain	264 – 676	413	Cytoplasmic Potential
	Repeat	98 – 122	25	LRR 1
	Repeat	123 – 146	24	LRR 2
	Repeat	148 – 171	24	LRR 3
	Repeat	172 – 195	24	LRR 4
	Repeat	197 – 218	22	LRR 5
	Domain	366 – 640	275	Protein kinase
	Nucleotide binding	372 – 380	9	ATP By similarity
Sites
	Binding site	394	1	ATP By similarity
Amino acid modifications
	Glycosylation	42	1	N-linked (GlcNAc...) Potential
	Glycosylation	73	1	N-linked (GlcNAc...) Potential
	Glycosylation	83	1	N-linked (GlcNAc...) Potential
	Glycosylation	218	1	N-linked (GlcNAc...) Potential
Natural variations
	Alternative sequence	1 – 88	88	Missing in isoform 2.		VSP_038293
	Alternative sequence	89 – 98	10	ELDVQALGSI → MERSDVFKRL in isoform 2.		VSP_038294
Experimental info
	Sequence conflict	487 – 488	2	DL → RI in BAF00232. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 65162EF1D0782579	FASTA	676	75,235
	10 20 30 40 50 60 MTRDDKFPIV YSLLLIVLLF VSPIYGDGDA DALLKFKSSL VNASSLGGWD SGEPPCSGDK 70 80 90 100 110 120 GSDSKWKGVM CSNGSVFALR LENMSLSGEL DVQALGSIRG LKSISFMRNH FEGKIPRGID 130 140 150 160 170 180 GLVSLAHLYL AHNQFTGEID GDLFSGMKAL LKVHLEGNRF SGEIPESLGK LPKLTELNLE 190 200 210 220 230 240 DNMFTGKIPA FKQKNLVTVN VANNQLEGRI PLTLGLMNIT FFSGNKGLCG APLLPCRYTR 250 260 270 280 290 300 PPFFTVFLLA LTILAVVVLI TVFLSVCILS RRQGKGQDQI QNHGVGHFHG QVYGQPEQQQ 310 320 330 340 350 360 HSEKSSQDSK VYRKLANETV QRDSTATSGA ISVGGLSPDE DKRGDQRKLH FVRNDQERFT 370 380 390 400 410 420 LQDMLRASAE VLGSGGFGSS YKAALSSGRA VVVKRFRFMS NIGREEFYDH MKKIGRLSHP 430 440 450 460 470 480 NLLPLIAFYY RKEEKLLVTN YISNGSLANL LHANRTPGQV VLDWPIRLKI VRGVTRGLAY 490 500 510 520 530 540 LYRVFPDLNL PHGHLKSSNV LLDPNFEPLL TDYALVPVVN RDQSQQFMVA YKAPEFTQQD 550 560 570 580 590 600 RTSRRSDVWS LGILILEILT GKFPANYLRQ GKGADDELAA WVESVARTEW TADVFDKEMK 610 620 630 640 650 660 AGKEHEAQML KLLKIGLRCC DWDIEKRIEL HEAVDRIEEV DRDAGGGQES VRSSYVTASD 670 GDHRSSRAMT EEFSLM
	Isoform 2 [UniParc]. Checksum: A2CA3D49FE525576	FASTA	588	66,126
	10 20 30 40 50 60 MERSDVFKRL RGLKSISFMR NHFEGKIPRG IDGLVSLAHL YLAHNQFTGE IDGDLFSGMK 70 80 90 100 110 120 ALLKVHLEGN RFSGEIPESL GKLPKLTELN LEDNMFTGKI PAFKQKNLVT VNVANNQLEG 130 140 150 160 170 180 RIPLTLGLMN ITFFSGNKGL CGAPLLPCRY TRPPFFTVFL LALTILAVVV LITVFLSVCI 190 200 210 220 230 240 LSRRQGKGQD QIQNHGVGHF HGQVYGQPEQ QQHSEKSSQD SKVYRKLANE TVQRDSTATS 250 260 270 280 290 300 GAISVGGLSP DEDKRGDQRK LHFVRNDQER FTLQDMLRAS AEVLGSGGFG SSYKAALSSG 310 320 330 340 350 360 RAVVVKRFRF MSNIGREEFY DHMKKIGRLS HPNLLPLIAF YYRKEEKLLV TNYISNGSLA 370 380 390 400 410 420 NLLHANRTPG QVVLDWPIRL KIVRGVTRGL AYLYRVFPDL NLPHGHLKSS NVLLDPNFEP 430 440 450 460 470 480 LLTDYALVPV VNRDQSQQFM VAYKAPEFTQ QDRTSRRSDV WSLGILILEI LTGKFPANYL 490 500 510 520 530 540 RQGKGADDEL AAWVESVART EWTADVFDKE MKAGKEHEAQ MLKLLKIGLR CCDWDIEKRI 550 560 570 580 ELHEAVDRIE EVDRDAGGGQ ESVRSSYVTA SDGDHRSSRA MTEEFSLM

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[4]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Strain: cv. Columbia.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL021633 Genomic DNA. Translation: CAA16528.1. Sequence problems. AL161578 Genomic DNA. Translation: CAB79843.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE85882.1. AK176198 mRNA. Translation: BAD43961.1. AK176233 mRNA. Translation: BAD43996.1. AK176245 mRNA. Translation: BAD44008.1. AK176283 mRNA. Translation: BAD44046.1. AK176290 mRNA. Translation: BAD44053.1. AK176304 mRNA. Translation: BAD44067.1. AK176329 mRNA. Translation: BAD44092.1. AK221228 mRNA. Translation: BAD93819.1. AK228286 mRNA. Translation: BAF00232.1. FJ708761 mRNA. Translation: ACN59354.1.
IPI	IPI00526638. IPI00953812.
PIR	T04492.
RefSeq	NP_567870.1. NM_119274.4.
UniGene	At.31759.
3D structure databases
ProteinModelPortal	C0LGR9.
SMR	C0LGR9. Positions 29-221, 288-638.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G31250.1; AT4G31250.1; AT4G31250.
GeneID	829252.
KEGG	ath:AT4G31250.
Organism-specific databases
GeneFarm	2307. 47.
TAIR	At4g31250.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000116554.
InParanoid	O49575.
OMA	LEVECDS.
PhylomeDB	C0LGR9.
ProtClustDB	CLSN2917641.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. [Graphical view]
Pfam	PF08263. LRRNT_2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y4312_ARATH

Accession

Primary (citable) accession number: C0LGR9
Secondary accession number(s): O49575, Q0WRL7, Q67Z85

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 33 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program