C0LGR3 (Y4265_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable LRR receptor-like serine/threonine-protein kinase At4g26540
EC=2.7.11.1

Gene names

Ordered Locus Names:	At4g26540
ORF Names:	M3E9.30

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 27 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence AAL32011.1 differs from that shown. Reason: Frameshift at position 1084. The sequence CAA18216.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAA18216.1 differs from that shown. Reason: Frameshift at position 931. The sequence CAB79509.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB79509.1 differs from that shown. Reason: Frameshift at position 931.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay Ref.5. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24	Potential
	Chain	25 – 1091	1067	Probable LRR receptor-like serine/threonine-protein kinase At4g26540		PRO_0000387553
Regions
	Topological domain	25 – 703	679	Extracellular Potential
	Transmembrane	704 – 724	21	Helical; Potential
	Topological domain	725 – 1091	367	Cytoplasmic Potential
	Repeat	33 – 56	24	LRR 1
	Repeat	67 – 91	25	LRR 2
	Repeat	92 – 115	24	LRR 3
	Repeat	116 – 140	25	LRR 4
	Repeat	142 – 166	25	LRR 5
	Repeat	168 – 188	21	LRR 6
	Repeat	190 – 213	24	LRR 7
	Repeat	214 – 237	24	LRR 8
	Repeat	239 – 261	23	LRR 9
	Repeat	262 – 285	24	LRR 10
	Repeat	287 – 309	23	LRR 11
	Repeat	311 – 332	22	LRR 12
	Repeat	333 – 357	25	LRR 13
	Repeat	359 – 383	25	LRR 14
	Repeat	385 – 405	21	LRR 15
	Repeat	406 – 429	24	LRR 16
	Repeat	431 – 453	23	LRR 17
	Repeat	454 – 477	24	LRR 18
	Repeat	478 – 501	24	LRR 19
	Repeat	503 – 524	22	LRR 20
	Repeat	525 – 548	24	LRR 21
	Repeat	549 – 572	24	LRR 22
	Repeat	574 – 596	23	LRR 23
	Repeat	598 – 620	23	LRR 24
	Repeat	621 – 644	24	LRR 25
	Repeat	645 – 668	24	LRR 26
	Repeat	669 – 690	22	LRR 27
	Domain	760 – 1046	287	Protein kinase
	Nucleotide binding	766 – 774	9	ATP By similarity
Sites
	Active site	883	1	Proton acceptor By similarity
	Binding site	788	1	ATP By similarity
Amino acid modifications
	Modified residue	762	1	Phosphoserine Ref.5
	Glycosylation	40	1	N-linked (GlcNAc...) Potential
	Glycosylation	104	1	N-linked (GlcNAc...) Potential
	Glycosylation	163	1	N-linked (GlcNAc...) Potential
	Glycosylation	356	1	N-linked (GlcNAc...) Potential
	Glycosylation	431	1	N-linked (GlcNAc...) Potential
	Glycosylation	452	1	N-linked (GlcNAc...) Potential
	Glycosylation	604	1	N-linked (GlcNAc...) Potential
	Glycosylation	651	1	N-linked (GlcNAc...) Potential
	Glycosylation	697	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	221	1	L → P in AAL32011. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	C0LGR3 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 061EFB0EDF281988	FASTA	1,091	119,529
	10 20 30 40 50 60 MPPNIYRLSF FSSLLCFFFI PCFSLDQQGQ ALLSWKSQLN ISGDAFSSWH VADTSPCNWV 70 80 90 100 110 120 GVKCNRRGEV SEIQLKGMDL QGSLPVTSLR SLKSLTSLTL SSLNLTGVIP KEIGDFTELE 130 140 150 160 170 180 LLDLSDNSLS GDIPVEIFRL KKLKTLSLNT NNLEGHIPME IGNLSGLVEL MLFDNKLSGE 190 200 210 220 230 240 IPRSIGELKN LQVLRAGGNK NLRGELPWEI GNCENLVMLG LAETSLSGKL PASIGNLKRV 250 260 270 280 290 300 QTIAIYTSLL SGPIPDEIGY CTELQNLYLY QNSISGSIPT TIGGLKKLQS LLLWQNNLVG 310 320 330 340 350 360 KIPTELGNCP ELWLIDFSEN LLTGTIPRSF GKLENLQELQ LSVNQISGTI PEELTNCTKL 370 380 390 400 410 420 THLEIDNNLI TGEIPSLMSN LRSLTMFFAW QNKLTGNIPQ SLSQCRELQA IDLSYNSLSG 430 440 450 460 470 480 SIPKEIFGLR NLTKLLLLSN DLSGFIPPDI GNCTNLYRLR LNGNRLAGSI PSEIGNLKNL 490 500 510 520 530 540 NFVDISENRL VGSIPPAISG CESLEFLDLH TNSLSGSLLG TTLPKSLKFI DFSDNALSST 550 560 570 580 590 600 LPPGIGLLTE LTKLNLAKNR LSGEIPREIS TCRSLQLLNL GENDFSGEIP DELGQIPSLA 610 620 630 640 650 660 ISLNLSCNRF VGEIPSRFSD LKNLGVLDVS HNQLTGNLNV LTDLQNLVSL NISYNDFSGD 670 680 690 700 710 720 LPNTPFFRRL PLSDLASNRG LYISNAISTR PDPTTRNSSV VRLTILILVV VTAVLVLMAV 730 740 750 760 770 780 YTLVRARAAG KQLLGEEIDS WEVTLYQKLD FSIDDIVKNL TSANVIGTGS SGVVYRITIP 790 800 810 820 830 840 SGESLAVKKM WSKEESGAFN SEIKTLGSIR HRNIVRLLGW CSNRNLKLLF YDYLPNGSLS 850 860 870 880 890 900 SRLHGAGKGG CVDWEARYDV VLGVAHALAY LHHDCLPTII HGDVKAMNVL LGPHFEPYLA 910 920 930 940 950 960 DFGLARTISG YPNTGIDLAK PTNRPPMAGS YGYMAPEHAS MQRITEKSDV YSYGVVLLEV 970 980 990 1000 1010 1020 LTGKHPLDPD LPGGAHLVKW VRDHLAEKKD PSRLLDPRLD GRTDSIMHEM LQTLAVAFLC 1030 1040 1050 1060 1070 1080 VSNKANERPL MKDVVAMLTE IRHIDVGRSE TEKIKAGGCG SKEPQQFMSN EKIINSHGSS 1090 NCSFAFSDDS V

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana." Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T., Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B., Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M., de Simone V., Obermaier B. , Mache R., Mueller M., Kreis M., Delseny M., Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D., Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J., Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B., Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J., Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R., Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M., Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P., Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S., Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C., Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J., Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S., Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A., Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M., Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D., Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E., Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S., Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R., Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M., Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E., Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P., Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K., Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K., de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K., Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M., Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G., Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K., Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K., Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W., Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H., Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B., Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J., Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K., O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N., Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A., Martienssen R., McCombie W.R. Nature 402:769-777(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], PHOSPHORYLATION AT SER-762, SUBCELLULAR LOCATION. Strain: cv. Columbia.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL022223 Genomic DNA. Translation: CAA18216.1. Sequence problems. AL161565 Genomic DNA. Translation: CAB79509.1. Sequence problems. CP002687 Genomic DNA. Translation: AEE85216.1. AF436829 mRNA. Translation: AAL32011.1. Frameshift. FJ708754 mRNA. Translation: ACN59348.1.
IPI	IPI00519446.
PIR	T05050.
RefSeq	NP_567748.5. NM_118787.5.
UniGene	At.20959.
3D structure databases
ProteinModelPortal	C0LGR3.
SMR	C0LGR3. Positions 27-1038.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT4G26540.1; AT4G26540.1; AT4G26540.
GeneID	828760.
KEGG	ath:AT4G26540.
Organism-specific databases
GeneFarm	624. 55.
TAIR	At4g26540.
Phylogenomic databases
eggNOG	COG4886.
HOGENOM	HOG000116551.
InParanoid	Q8W556.
OMA	KANERPL.
PhylomeDB	C0LGR3.
ProtClustDB	CLSN2927436.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. PF08263. LRRNT_2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y4265_ARATH

Accession

Primary (citable) accession number: C0LGR3
Secondary accession number(s): O65580, Q8W556

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program