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Protein

Probable leucine-rich repeat receptor-like protein kinase IMK3

Gene

IMK3

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Can phosphorylate AGL24.1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei516ATPPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi494 – 502ATPPROSITE-ProRule annotation9

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Probable leucine-rich repeat receptor-like protein kinase IMK3 (EC:2.7.11.1)
Alternative name(s):
Protein INFLORESCENCE MERISTEM RECEPTOR-LIKE KINASE 3
Protein MERISTEMATIC RECEPTOR-LIKE KINASE
Gene namesi
Name:IMK3
Synonyms:MRLK
Ordered Locus Names:At3g56100
ORF Names:F18O21.60
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 3

Organism-specific databases

TAIRiAT3G56100.

Subcellular locationi

  • Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini49 – 409ExtracellularSequence analysisAdd BLAST361
Transmembranei410 – 430HelicalSequence analysisAdd BLAST21
Topological domaini431 – 784CytoplasmicSequence analysisAdd BLAST354

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 48Sequence analysisAdd BLAST48
ChainiPRO_000038945849 – 784Probable leucine-rich repeat receptor-like protein kinase IMK3Add BLAST736

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi82N-linked (GlcNAc...)Sequence analysis1
Glycosylationi203N-linked (GlcNAc...)Sequence analysis1
Glycosylationi232N-linked (GlcNAc...)Sequence analysis1
Glycosylationi268N-linked (GlcNAc...)Sequence analysis1
Glycosylationi316N-linked (GlcNAc...)Sequence analysis1
Glycosylationi348N-linked (GlcNAc...)Sequence analysis1
Glycosylationi353N-linked (GlcNAc...)Sequence analysis1
Glycosylationi367N-linked (GlcNAc...)Sequence analysis1
Glycosylationi404N-linked (GlcNAc...)Sequence analysis1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiC0LGP9.

Expressioni

Tissue specificityi

Expressed in meristems, including roots, vegetative, inflorescence and floral meristems, and in embryos.2 Publications

Interactioni

Subunit structurei

Interacts with AGL24.1 Publication

Protein-protein interaction databases

BioGridi10092. 3 interactors.

Structurei

3D structure databases

ProteinModelPortaliC0LGP9.
SMRiC0LGP9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati126 – 148LRR 1Add BLAST23
Repeati150 – 172LRR 2Add BLAST23
Repeati174 – 197LRR 3Add BLAST24
Repeati198 – 220LRR 4Add BLAST23
Repeati222 – 242LRR 5Add BLAST21
Repeati247 – 268LRR 6Add BLAST22
Repeati271 – 294LRR 7Add BLAST24
Repeati295 – 317LRR 8Add BLAST23
Repeati319 – 342LRR 9Add BLAST24
Repeati343 – 365LRR 10Add BLAST23
Domaini488 – 773Protein kinasePROSITE-ProRule annotationAdd BLAST286

Domaini

The protein kinase domain is predicted to be catalytically inactive. Lacks the conserved Asp active site at position 612, which is replaced by an Asn residue. However, according to Ref. 9, the kinase activity is conserved.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 10 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

InParanoidiC0LGP9.
PhylomeDBiC0LGP9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 9 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0LGP9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEFITQNQAI TSLSMINTDI DQPKASLRSR FLLHLIICLL FFVPPCSSQA
60 70 80 90 100
WDGVVITQAD YQGLQAVKQE LIDPRGFLRS WNGSGFSACS GGWAGIKCAQ
110 120 130 140 150
GQVIVIQLPW KSLGGRISEK IGQLQALRKL SLHDNNLGGS IPMSLGLIPN
160 170 180 190 200
LRGVQLFNNR LTGSIPASLG VSHFLQTLDL SNNLLSEIIP PNLADSSKLL
210 220 230 240 250
RLNLSFNSLS GQIPVSLSRS SSLQFLALDH NNLSGPILDT WGSKSLNLRV
260 270 280 290 300
LSLDHNSLSG PFPFSLCNLT QLQDFSFSHN RIRGTLPSEL SKLTKLRKMD
310 320 330 340 350
ISGNSVSGHI PETLGNISSL IHLDLSQNKL TGEIPISISD LESLNFFNVS
360 370 380 390 400
YNNLSGPVPT LLSQKFNSSS FVGNSLLCGY SVSTPCPTLP SPSPEKERKP
410 420 430 440 450
SHRNLSTKDI ILIASGALLI VMLILVCVLC CLLRKKANET KAKGGEAGPG
460 470 480 490 500
AVAAKTEKGG EAEAGGETGG KLVHFDGPMA FTADDLLCAT AEIMGKSTYG
510 520 530 540 550
TVYKATLEDG SQVAVKRLRE KITKSQKEFE NEINVLGRIR HPNLLALRAY
560 570 580 590 600
YLGPKGEKLV VFDYMSRGSL ATFLHARGPD VHINWPTRMS LIKGMARGLF
610 620 630 640 650
YLHTHANIIH GNLTSSNVLL DENITAKISD YGLSRLMTAA AGSSVIATAG
660 670 680 690 700
ALGYRAPELS KLKKANTKTD VYSLGVIILE LLTGKSPSEA LNGVDLPQWV
710 720 730 740 750
ATAVKEEWTN EVFDLELLND VNTMGDEILN TLKLALHCVD ATPSTRPEAQ
760 770 780
QVMTQLGEIR PEETTATTSE PLIDVPEASA STSQ
Length:784
Mass (Da):84,687
Last modified:May 5, 2009 - v1
Checksum:i6F9B3F69661F2CFF
GO

Sequence cautioni

The sequence AEE79477 differs from that shown. Reason: Frameshift at positions 275 and 520.Curated
The sequence AEE79477 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence BAD94529 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAB87409 differs from that shown. Reason: Frameshift at positions 275 and 520.Curated
The sequence CAB87409 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163763 Genomic DNA. Translation: CAB87409.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79477.1. Sequence problems.
FJ708740 mRNA. Translation: ACN59334.1.
AK118661 mRNA. Translation: BAC43256.1.
AK221981 mRNA. Translation: BAD94529.1. Different initiation.
BT005241 mRNA. Translation: AAO63305.1.
PIRiT47727.
UniGeneiAt.34964.

Genome annotation databases

KEGGiath:AT3G56100.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL163763 Genomic DNA. Translation: CAB87409.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79477.1. Sequence problems.
FJ708740 mRNA. Translation: ACN59334.1.
AK118661 mRNA. Translation: BAC43256.1.
AK221981 mRNA. Translation: BAD94529.1. Different initiation.
BT005241 mRNA. Translation: AAO63305.1.
PIRiT47727.
UniGeneiAt.34964.

3D structure databases

ProteinModelPortaliC0LGP9.
SMRiC0LGP9.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi10092. 3 interactors.

Proteomic databases

PRIDEiC0LGP9.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiath:AT3G56100.

Organism-specific databases

TAIRiAT3G56100.

Phylogenomic databases

InParanoidiC0LGP9.
PhylomeDBiC0LGP9.

Miscellaneous databases

PROiC0LGP9.

Family and domain databases

Gene3Di2.60.120.200. 1 hit.
3.80.10.10. 1 hit.
InterProiIPR013320. ConA-like_dom.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR001611. Leu-rich_rpt.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamiPF13855. LRR_8. 2 hits.
PF08263. LRRNT_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS51450. LRR. 9 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMK3_ARATH
AccessioniPrimary (citable) accession number: C0LGP9
Secondary accession number(s): F4IY70
, Q56WQ4, Q8GWS6, Q9LYN1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 5, 2009
Last modified: November 30, 2016
This is version 52 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.