C0LGP9 (IMK3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable leucine-rich repeat receptor-like protein kinase IMK3
EC=2.7.11.1

Alternative name(s):
Protein INFLORESCENCE MERISTEM RECEPTOR-LIKE KINASE 3
Protein MERISTEMATIC RECEPTOR-LIKE KINASE

Gene names

Name:	IMK3
Synonyms:	MRLK
Ordered Locus Names:	At3g56100
ORF Names:	F18O21.60

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	784 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Can phosphorylate AGL24. Ref.9
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with AGL24. Ref.9
Subcellular location	Cell membrane; Single-pass type I membrane protein Ref.8.
Tissue specificity	Expressed in meristems, including roots, vegetative, inflorescence and floral meristems, and in embryos. Ref.7 Ref.8
Domain	The protein kinase domain is predicted to be catalytically inactive. Lacks the conserved Asp active site at position 612, which is replaced by an Asn residue. However, according to Ref.9, the kinase activity is conserved.
Post-translational modification	Autophosphorylated. Ref.9
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 10 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence AEE79477.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AEE79477.1 differs from that shown. Reason: Frameshift at positions 275 and 520. The sequence BAD94529.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB87409.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence CAB87409.1 differs from that shown. Reason: Frameshift at positions 275 and 520.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 48	48	Potential
	Chain	49 – 784	736	Probable leucine-rich repeat receptor-like protein kinase IMK3		PRO_0000389458
Regions
	Topological domain	49 – 409	361	Extracellular Potential
	Transmembrane	410 – 430	21	Helical; Potential
	Topological domain	431 – 784	354	Cytoplasmic Potential
	Repeat	126 – 148	23	LRR 1
	Repeat	150 – 172	23	LRR 2
	Repeat	174 – 197	24	LRR 3
	Repeat	198 – 220	23	LRR 4
	Repeat	222 – 242	21	LRR 5
	Repeat	247 – 268	22	LRR 6
	Repeat	271 – 294	24	LRR 7
	Repeat	295 – 317	23	LRR 8
	Repeat	319 – 342	24	LRR 9
	Repeat	343 – 365	23	LRR 10
	Domain	488 – 773	286	Protein kinase
	Nucleotide binding	494 – 502	9	ATP By similarity
Sites
	Binding site	516	1	ATP By similarity
Amino acid modifications
	Glycosylation	82	1	N-linked (GlcNAc...) Potential
	Glycosylation	203	1	N-linked (GlcNAc...) Potential
	Glycosylation	232	1	N-linked (GlcNAc...) Potential
	Glycosylation	268	1	N-linked (GlcNAc...) Potential
	Glycosylation	316	1	N-linked (GlcNAc...) Potential
	Glycosylation	348	1	N-linked (GlcNAc...) Potential
	Glycosylation	353	1	N-linked (GlcNAc...) Potential
	Glycosylation	367	1	N-linked (GlcNAc...) Potential
	Glycosylation	404	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	C0LGP9 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 6F9B3F69661F2CFF	FASTA	784	84,687
	10 20 30 40 50 60 MEFITQNQAI TSLSMINTDI DQPKASLRSR FLLHLIICLL FFVPPCSSQA WDGVVITQAD 70 80 90 100 110 120 YQGLQAVKQE LIDPRGFLRS WNGSGFSACS GGWAGIKCAQ GQVIVIQLPW KSLGGRISEK 130 140 150 160 170 180 IGQLQALRKL SLHDNNLGGS IPMSLGLIPN LRGVQLFNNR LTGSIPASLG VSHFLQTLDL 190 200 210 220 230 240 SNNLLSEIIP PNLADSSKLL RLNLSFNSLS GQIPVSLSRS SSLQFLALDH NNLSGPILDT 250 260 270 280 290 300 WGSKSLNLRV LSLDHNSLSG PFPFSLCNLT QLQDFSFSHN RIRGTLPSEL SKLTKLRKMD 310 320 330 340 350 360 ISGNSVSGHI PETLGNISSL IHLDLSQNKL TGEIPISISD LESLNFFNVS YNNLSGPVPT 370 380 390 400 410 420 LLSQKFNSSS FVGNSLLCGY SVSTPCPTLP SPSPEKERKP SHRNLSTKDI ILIASGALLI 430 440 450 460 470 480 VMLILVCVLC CLLRKKANET KAKGGEAGPG AVAAKTEKGG EAEAGGETGG KLVHFDGPMA 490 500 510 520 530 540 FTADDLLCAT AEIMGKSTYG TVYKATLEDG SQVAVKRLRE KITKSQKEFE NEINVLGRIR 550 560 570 580 590 600 HPNLLALRAY YLGPKGEKLV VFDYMSRGSL ATFLHARGPD VHINWPTRMS LIKGMARGLF 610 620 630 640 650 660 YLHTHANIIH GNLTSSNVLL DENITAKISD YGLSRLMTAA AGSSVIATAG ALGYRAPELS 670 680 690 700 710 720 KLKKANTKTD VYSLGVIILE LLTGKSPSEA LNGVDLPQWV ATAVKEEWTN EVFDLELLND 730 740 750 760 770 780 VNTMGDEILN TLKLALHCVD ATPSTRPEAQ QVMTQLGEIR PEETTATTSE PLIDVPEASA STSQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana." Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 562-784. Strain: cv. Columbia.
[5]	"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs." Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K. Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 562-784. Strain: cv. Columbia.
[6]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 565-784. Strain: cv. Columbia.
[7]	"Structural and functional analysis of the receptor-like kinase genes expressed at Arabidopsis inflorescence." Takemura M., Kohchi T., Kanamoto H., Asai T., Nemoto K., Yokota A. (In) Proceedings of the 9th international conference on Arabidopsis research, abstract#501708527, Madison (1998) Cited for: TISSUE SPECIFICITY.
[8]	"Analysis of the receptor-like kinase gene expressed in the meristems." Takemura M., Kanamoto H., Hasegawa M., Yokota A., Kohchi T. (In) Proceedings of the 10th international conference on Arabidopsis research, abstract#12-15, Melbourne (1999) Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[9]	"Functional analysis of the receptor-like kinase gene expressed in both shoot and root apical meristems." Takemura M., Tani E., Takeda Y., Yokota A., Kohchi T. (In) Proceedings of the 11th international conference on Arabidopsis research, abstract#295, Madison (2000) Cited for: FUNCTION, AUTOPHOSPHORYLATION, INTERACTION WITH AGL24, ACTIVITY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL163763 Genomic DNA. Translation: CAB87409.1. Sequence problems. CP002686 Genomic DNA. Translation: AEE79477.1. Sequence problems. FJ708740 mRNA. Translation: ACN59334.1. AK118661 mRNA. Translation: BAC43256.1. AK221981 mRNA. Translation: BAD94529.1. Different initiation. BT005241 mRNA. Translation: AAO63305.1.
IPI	IPI00929972. IPI01020340.
PIR	T47727.
RefSeq	NP_191169.1. NM_115468.2.
UniGene	At.34964.
3D structure databases
ProteinModelPortal	C0LGP9.
SMR	C0LGP9. Positions 60-388, 458-756.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT3G56100.1-P.
Proteomic databases
PRIDE	C0LGP9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	824776.
KEGG	ath:AT3G56100.
Organism-specific databases
GeneFarm	2317. 47.
TAIR	At3g56100.
Phylogenomic databases
InParanoid	Q9LYN1.
PhylomeDB	C0LGP9.
ProtClustDB	CLSN2913434.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF00560. LRR_1. 3 hits. PF08263. LRRNT_2. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS51450. LRR. 9 hits. PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IMK3_ARATH

Accession

Primary (citable) accession number: C0LGP9
Secondary accession number(s): F4IY70 , Q56WQ4, Q8GWS6, Q9LYN1

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 24, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program