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C0LGP9 (IMK3_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 40. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable leucine-rich repeat receptor-like protein kinase IMK3

EC=2.7.11.1
Alternative name(s):
Protein INFLORESCENCE MERISTEM RECEPTOR-LIKE KINASE 3
Protein MERISTEMATIC RECEPTOR-LIKE KINASE
Gene names
Name:IMK3
Synonyms:MRLK
Ordered Locus Names:At3g56100
ORF Names:F18O21.60
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length784 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Can phosphorylate AGL24. Ref.9

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with AGL24. Ref.9

Subcellular location

Cell membrane; Single-pass type I membrane protein Ref.8.

Tissue specificity

Expressed in meristems, including roots, vegetative, inflorescence and floral meristems, and in embryos. Ref.7 Ref.8

Domain

The protein kinase domain is predicted to be catalytically inactive. Lacks the conserved Asp active site at position 612, which is replaced by an Asn residue. However, according to Ref.9, the kinase activity is conserved.

Post-translational modification

Autophosphorylated. Ref.9

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 10 LRR (leucine-rich) repeats.

Contains 1 protein kinase domain.

Sequence caution

The sequence AEE79477.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AEE79477.1 differs from that shown. Reason: Frameshift at positions 275 and 520.

The sequence BAD94529.1 differs from that shown. Reason: Erroneous initiation.

The sequence CAB87409.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence CAB87409.1 differs from that shown. Reason: Frameshift at positions 275 and 520.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 4848 Potential
Chain49 – 784736Probable leucine-rich repeat receptor-like protein kinase IMK3
PRO_0000389458

Regions

Topological domain49 – 409361Extracellular Potential
Transmembrane410 – 43021Helical; Potential
Topological domain431 – 784354Cytoplasmic Potential
Repeat126 – 14823LRR 1
Repeat150 – 17223LRR 2
Repeat174 – 19724LRR 3
Repeat198 – 22023LRR 4
Repeat222 – 24221LRR 5
Repeat247 – 26822LRR 6
Repeat271 – 29424LRR 7
Repeat295 – 31723LRR 8
Repeat319 – 34224LRR 9
Repeat343 – 36523LRR 10
Domain488 – 773286Protein kinase
Nucleotide binding494 – 5029ATP By similarity

Sites

Binding site5161ATP By similarity

Amino acid modifications

Glycosylation821N-linked (GlcNAc...) Potential
Glycosylation2031N-linked (GlcNAc...) Potential
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2681N-linked (GlcNAc...) Potential
Glycosylation3161N-linked (GlcNAc...) Potential
Glycosylation3481N-linked (GlcNAc...) Potential
Glycosylation3531N-linked (GlcNAc...) Potential
Glycosylation3671N-linked (GlcNAc...) Potential
Glycosylation4041N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
C0LGP9 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: 6F9B3F69661F2CFF

FASTA78484,687
        10         20         30         40         50         60 
MEFITQNQAI TSLSMINTDI DQPKASLRSR FLLHLIICLL FFVPPCSSQA WDGVVITQAD 

        70         80         90        100        110        120 
YQGLQAVKQE LIDPRGFLRS WNGSGFSACS GGWAGIKCAQ GQVIVIQLPW KSLGGRISEK 

       130        140        150        160        170        180 
IGQLQALRKL SLHDNNLGGS IPMSLGLIPN LRGVQLFNNR LTGSIPASLG VSHFLQTLDL 

       190        200        210        220        230        240 
SNNLLSEIIP PNLADSSKLL RLNLSFNSLS GQIPVSLSRS SSLQFLALDH NNLSGPILDT 

       250        260        270        280        290        300 
WGSKSLNLRV LSLDHNSLSG PFPFSLCNLT QLQDFSFSHN RIRGTLPSEL SKLTKLRKMD 

       310        320        330        340        350        360 
ISGNSVSGHI PETLGNISSL IHLDLSQNKL TGEIPISISD LESLNFFNVS YNNLSGPVPT 

       370        380        390        400        410        420 
LLSQKFNSSS FVGNSLLCGY SVSTPCPTLP SPSPEKERKP SHRNLSTKDI ILIASGALLI 

       430        440        450        460        470        480 
VMLILVCVLC CLLRKKANET KAKGGEAGPG AVAAKTEKGG EAEAGGETGG KLVHFDGPMA 

       490        500        510        520        530        540 
FTADDLLCAT AEIMGKSTYG TVYKATLEDG SQVAVKRLRE KITKSQKEFE NEINVLGRIR 

       550        560        570        580        590        600 
HPNLLALRAY YLGPKGEKLV VFDYMSRGSL ATFLHARGPD VHINWPTRMS LIKGMARGLF 

       610        620        630        640        650        660 
YLHTHANIIH GNLTSSNVLL DENITAKISD YGLSRLMTAA AGSSVIATAG ALGYRAPELS 

       670        680        690        700        710        720 
KLKKANTKTD VYSLGVIILE LLTGKSPSEA LNGVDLPQWV ATAVKEEWTN EVFDLELLND 

       730        740        750        760        770        780 
VNTMGDEILN TLKLALHCVD ATPSTRPEAQ QVMTQLGEIR PEETTATTSE PLIDVPEASA 


STSQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana."
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F. expand/collapse author list , Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
Nature 408:820-822(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Functional annotation of a full-length Arabidopsis cDNA collection."
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.
Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 562-784.
Strain: cv. Columbia.
[5]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 562-784.
Strain: cv. Columbia.
[6]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 565-784.
Strain: cv. Columbia.
[7]"Structural and functional analysis of the receptor-like kinase genes expressed at Arabidopsis inflorescence."
Takemura M., Kohchi T., Kanamoto H., Asai T., Nemoto K., Yokota A.
(In) Proceedings of the 9th international conference on Arabidopsis research, abstract#501708527, Madison (1998)
Cited for: TISSUE SPECIFICITY.
[8]"Analysis of the receptor-like kinase gene expressed in the meristems."
Takemura M., Kanamoto H., Hasegawa M., Yokota A., Kohchi T.
(In) Proceedings of the 10th international conference on Arabidopsis research, abstract#12-15, Melbourne (1999)
Cited for: TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
[9]"Functional analysis of the receptor-like kinase gene expressed in both shoot and root apical meristems."
Takemura M., Tani E., Takeda Y., Yokota A., Kohchi T.
(In) Proceedings of the 11th international conference on Arabidopsis research, abstract#295, Madison (2000)
Cited for: FUNCTION, AUTOPHOSPHORYLATION, INTERACTION WITH AGL24, ACTIVITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL163763 Genomic DNA. Translation: CAB87409.1. Sequence problems.
CP002686 Genomic DNA. Translation: AEE79477.1. Sequence problems.
FJ708740 mRNA. Translation: ACN59334.1.
AK118661 mRNA. Translation: BAC43256.1.
AK221981 mRNA. Translation: BAD94529.1. Different initiation.
BT005241 mRNA. Translation: AAO63305.1.
PIRT47727.
RefSeqNP_191169.1. NM_115468.2.
UniGeneAt.34964.

3D structure databases

ProteinModelPortalC0LGP9.
SMRC0LGP9. Positions 59-375, 458-760.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid10092. 3 interactions.
STRING3702.AT3G56100.1-P.

Proteomic databases

PRIDEC0LGP9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID824776.
KEGGath:AT3G56100.

Organism-specific databases

GeneFarm2317. 47.
TAIRAT3G56100.

Phylogenomic databases

InParanoidQ9LYN1.
PhylomeDBC0LGP9.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
[Graphical view]
PfamPF00560. LRR_1. 3 hits.
PF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS51450. LRR. 9 hits.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameIMK3_ARATH
AccessionPrimary (citable) accession number: C0LGP9
Secondary accession number(s): F4IY70 expand/collapse secondary AC list , Q56WQ4, Q8GWS6, Q9LYN1
Entry history
Integrated into UniProtKB/Swiss-Prot: November 24, 2009
Last sequence update: May 5, 2009
Last modified: June 11, 2014
This is version 40 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names