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C0LGL9

- FEI2_ARATH

UniProt

C0LGL9 - FEI2_ARATH

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Protein
LRR receptor-like serine/threonine-protein kinase FEI 2
Gene
FEI2, At2g35620, T20F21.18
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei332 – 3321ATP By similarity
Active sitei427 – 4271Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi310 – 3189ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. kinase activity Source: TAIR
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. plant-type cell wall organization Source: TAIR
  2. unidimensional cell growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT2G35620-MONOMER.
ARA:GQT-1659-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase FEI 2 (EC:2.7.11.1)
Gene namesi
Name:FEI2
Ordered Locus Names:At2g35620
ORF Names:T20F21.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 2

Organism-specific databases

TAIRiAT2G35620.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini29 – 236208Extracellular Reviewed prediction
Add
BLAST
Transmembranei237 – 25721Helical; Reviewed prediction
Add
BLAST
Topological domaini258 – 589332Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi336 – 3361K → R: Normal interaction with ACC synthases. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2828 Reviewed prediction
Add
BLAST
Chaini29 – 589561LRR receptor-like serine/threonine-protein kinase FEI 2
PRO_0000387512Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi119 – 1191N-linked (GlcNAc...) Reviewed prediction
Glycosylationi143 – 1431N-linked (GlcNAc...) Reviewed prediction
Glycosylationi175 – 1751N-linked (GlcNAc...) Reviewed prediction
Glycosylationi215 – 2151N-linked (GlcNAc...) Reviewed prediction
Glycosylationi219 – 2191N-linked (GlcNAc...) Reviewed prediction
Modified residuei384 – 3841Phosphoserine By similarity
Modified residuei460 – 4601Phosphothreonine By similarity
Modified residuei461 – 4611Phosphothreonine By similarity
Modified residuei466 – 4661Phosphothreonine By similarity
Modified residuei474 – 4741Phosphotyrosine By similarity

Post-translational modificationi

Autophosphorylated By similarity.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiC0LGL9.
PRIDEiC0LGL9.

Expressioni

Tissue specificityi

Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

Interactioni

Subunit structurei

Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

Protein-protein interaction databases

STRINGi3702.AT2G35620.1-P.

Structurei

3D structure databases

ProteinModelPortaliC0LGL9.
SMRiC0LGL9. Positions 32-207, 260-575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati72 – 9625LRR 1
Add
BLAST
Repeati97 – 12024LRR 2
Add
BLAST
Repeati122 – 14423LRR 3
Add
BLAST
Repeati145 – 16824LRR 4
Add
BLAST
Repeati170 – 19324LRR 5
Add
BLAST
Domaini304 – 576273Protein kinase
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000116554.
InParanoidiQ9ZQN5.
OMAiFNIVGWL.
PhylomeDBiC0LGL9.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0LGL9-1 [UniParc]FASTAAdd to Basket

« Hide

MGICLMKRCC SWFLLISFLS ALTNENEAIS PDGEALLSFR NGVLASDGVI    50
GLWRPEDPDP CNWKGVTCDA KTKRVIALSL TYHKLRGPLP PELGKLDQLR 100
LLMLHNNALY QSIPASLGNC TALEGIYLQN NYITGTIPSE IGNLSGLKNL 150
DLSNNNLNGA IPASLGQLKR LTKFNVSNNF LVGKIPSDGL LARLSRDSFN 200
GNRNLCGKQI DIVCNDSGNS TASGSPTGQG GNNPKRLLIS ASATVGGLLL 250
VALMCFWGCF LYKKLGRVES KSLVIDVGGG ASIVMFHGDL PYASKDIIKK 300
LESLNEEHII GCGGFGTVYK LSMDDGNVFA LKRIVKLNEG FDRFFERELE 350
ILGSIKHRYL VNLRGYCNSP TSKLLLYDYL PGGSLDEALH KRGEQLDWDS 400
RVNIIIGAAK GLAYLHHDCS PRIIHRDIKS SNILLDGNLE ARVSDFGLAK 450
LLEDEESHIT TIVAGTFGYL APEYMQSGRA TEKTDVYSFG VLVLEVLSGK 500
LPTDASFIEK GFNIVGWLNF LISENRAKEI VDLSCEGVER ESLDALLSIA 550
TKCVSSSPDE RPTMHRVVQL LESEVMTPCP SDFYDSSSD 589
Length:589
Mass (Da):64,355
Last modified:May 5, 2009 - v1
Checksum:iA3A78CA54E1E1400
GO

Sequence cautioni

The sequence AAD15451.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006068 Genomic DNA. Translation: AAD15451.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09131.1.
CP002685 Genomic DNA. Translation: AEC09132.1.
FJ708709 mRNA. Translation: ACN59304.1.
AK230094 mRNA. Translation: BAF01913.1.
PIRiH84770.
RefSeqiNP_001189684.1. NM_001202755.1.
NP_181105.2. NM_129116.4.
UniGeneiAt.37644.

Genome annotation databases

EnsemblPlantsiAT2G35620.1; AT2G35620.1; AT2G35620.
AT2G35620.2; AT2G35620.2; AT2G35620.
GeneIDi818130.
KEGGiath:AT2G35620.

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC006068 Genomic DNA. Translation: AAD15451.1 . Sequence problems.
CP002685 Genomic DNA. Translation: AEC09131.1 .
CP002685 Genomic DNA. Translation: AEC09132.1 .
FJ708709 mRNA. Translation: ACN59304.1 .
AK230094 mRNA. Translation: BAF01913.1 .
PIRi H84770.
RefSeqi NP_001189684.1. NM_001202755.1.
NP_181105.2. NM_129116.4.
UniGenei At.37644.

3D structure databases

ProteinModelPortali C0LGL9.
SMRi C0LGL9. Positions 32-207, 260-575.
ModBasei Search...

Protein-protein interaction databases

STRINGi 3702.AT2G35620.1-P.

Proteomic databases

PaxDbi C0LGL9.
PRIDEi C0LGL9.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT2G35620.1 ; AT2G35620.1 ; AT2G35620 .
AT2G35620.2 ; AT2G35620.2 ; AT2G35620 .
GeneIDi 818130.
KEGGi ath:AT2G35620.

Organism-specific databases

GeneFarmi 2548. 221.
TAIRi AT2G35620.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000116554.
InParanoidi Q9ZQN5.
OMAi FNIVGWL.
PhylomeDBi C0LGL9.

Enzyme and pathway databases

BioCyci ARA:AT2G35620-MONOMER.
ARA:GQT-1659-MONOMER.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
    Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
    BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
    Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
    , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
    Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-589.
    Strain: cv. Columbia.
  5. "Two leucine-rich repeat receptor kinases mediate signaling, linking cell wall biosynthesis and ACC synthase in Arabidopsis."
    Xu S.-L., Rahman A., Baskin T.I., Kieber J.J.
    Plant Cell 20:3065-3079(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-336, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH ACS5 AND ACS9.

Entry informationi

Entry nameiFEI2_ARATH
AccessioniPrimary (citable) accession number: C0LGL9
Secondary accession number(s): Q0WLU4, Q9ZQN5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'Fei' means fat in Chinese.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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