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C0LGL9 (FEI2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
LRR receptor-like serine/threonine-protein kinase FEI 2

EC=2.7.11.1
Gene names
Name:FEI2
Ordered Locus Names:At2g35620
ORF Names:T20F21.18
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length589 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene). Ref.5

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Subunit structure

Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain. Ref.5

Subcellular location

Cell membrane; Single-pass type I membrane protein Ref.5.

Tissue specificity

Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings. Ref.5

Post-translational modification

Autophosphorylated By similarity.

Disruption phenotype

No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis. Ref.5

Miscellaneous

'Fei' means fat in Chinese.

Sequence similarities

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.

Contains 5 LRR (leucine-rich) repeats.

Contains 1 protein kinase domain.

Sequence caution

The sequence AAD15451.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2828 Potential
Chain29 – 589561LRR receptor-like serine/threonine-protein kinase FEI 2
PRO_0000387512

Regions

Topological domain29 – 236208Extracellular Potential
Transmembrane237 – 25721Helical; Potential
Topological domain258 – 589332Cytoplasmic Potential
Repeat72 – 9625LRR 1
Repeat97 – 12024LRR 2
Repeat122 – 14423LRR 3
Repeat145 – 16824LRR 4
Repeat170 – 19324LRR 5
Domain304 – 576273Protein kinase
Nucleotide binding310 – 3189ATP By similarity

Sites

Active site4271Proton acceptor By similarity
Binding site3321ATP By similarity

Amino acid modifications

Modified residue3841Phosphoserine By similarity
Modified residue4601Phosphothreonine By similarity
Modified residue4611Phosphothreonine By similarity
Modified residue4661Phosphothreonine By similarity
Modified residue4741Phosphotyrosine By similarity
Glycosylation1191N-linked (GlcNAc...) Potential
Glycosylation1431N-linked (GlcNAc...) Potential
Glycosylation1751N-linked (GlcNAc...) Potential
Glycosylation2151N-linked (GlcNAc...) Potential
Glycosylation2191N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis3361K → R: Normal interaction with ACC synthases. Ref.5

Sequences

Sequence LengthMass (Da)Tools
C0LGL9 [UniParc].

Last modified May 5, 2009. Version 1.
Checksum: A3A78CA54E1E1400

FASTA58964,355
        10         20         30         40         50         60 
MGICLMKRCC SWFLLISFLS ALTNENEAIS PDGEALLSFR NGVLASDGVI GLWRPEDPDP 

        70         80         90        100        110        120 
CNWKGVTCDA KTKRVIALSL TYHKLRGPLP PELGKLDQLR LLMLHNNALY QSIPASLGNC 

       130        140        150        160        170        180 
TALEGIYLQN NYITGTIPSE IGNLSGLKNL DLSNNNLNGA IPASLGQLKR LTKFNVSNNF 

       190        200        210        220        230        240 
LVGKIPSDGL LARLSRDSFN GNRNLCGKQI DIVCNDSGNS TASGSPTGQG GNNPKRLLIS 

       250        260        270        280        290        300 
ASATVGGLLL VALMCFWGCF LYKKLGRVES KSLVIDVGGG ASIVMFHGDL PYASKDIIKK 

       310        320        330        340        350        360 
LESLNEEHII GCGGFGTVYK LSMDDGNVFA LKRIVKLNEG FDRFFERELE ILGSIKHRYL 

       370        380        390        400        410        420 
VNLRGYCNSP TSKLLLYDYL PGGSLDEALH KRGEQLDWDS RVNIIIGAAK GLAYLHHDCS 

       430        440        450        460        470        480 
PRIIHRDIKS SNILLDGNLE ARVSDFGLAK LLEDEESHIT TIVAGTFGYL APEYMQSGRA 

       490        500        510        520        530        540 
TEKTDVYSFG VLVLEVLSGK LPTDASFIEK GFNIVGWLNF LISENRAKEI VDLSCEGVER 

       550        560        570        580 
ESLDALLSIA TKCVSSSPDE RPTMHRVVQL LESEVMTPCP SDFYDSSSD 

« Hide

References

« Hide 'large scale' references
[1]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[2]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[3]"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
[4]"Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K. expand/collapse author list , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-589.
Strain: cv. Columbia.
[5]"Two leucine-rich repeat receptor kinases mediate signaling, linking cell wall biosynthesis and ACC synthase in Arabidopsis."
Xu S.-L., Rahman A., Baskin T.I., Kieber J.J.
Plant Cell 20:3065-3079(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LYS-336, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH ACS5 AND ACS9.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AC006068 Genomic DNA. Translation: AAD15451.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC09131.1.
CP002685 Genomic DNA. Translation: AEC09132.1.
FJ708709 mRNA. Translation: ACN59304.1.
AK230094 mRNA. Translation: BAF01913.1.
PIRH84770.
RefSeqNP_001189684.1. NM_001202755.1.
NP_181105.2. NM_129116.4.
UniGeneAt.37644.

3D structure databases

ProteinModelPortalC0LGL9.
SMRC0LGL9. Positions 32-207, 260-575.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING3702.AT2G35620.1-P.

Proteomic databases

PaxDbC0LGL9.
PRIDEC0LGL9.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G35620.1; AT2G35620.1; AT2G35620.
AT2G35620.2; AT2G35620.2; AT2G35620.
GeneID818130.
KEGGath:AT2G35620.

Organism-specific databases

GeneFarm2548. 221.
TAIRAT2G35620.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000116554.
InParanoidQ9ZQN5.
OMAFNIVGWL.
PhylomeDBC0LGL9.

Enzyme and pathway databases

BioCycARA:AT2G35620-MONOMER.
ARA:GQT-1659-MONOMER.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF13855. LRR_8. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFEI2_ARATH
AccessionPrimary (citable) accession number: C0LGL9
Secondary accession number(s): Q0WLU4, Q9ZQN5
Entry history
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: May 14, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names