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C0LGL9

- FEI2_ARATH

UniProt

C0LGL9 - FEI2_ARATH

Protein

LRR receptor-like serine/threonine-protein kinase FEI 2

Gene

FEI2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 44 (01 Oct 2014)
      Sequence version 1 (05 May 2009)
      Previous versions | rss
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    Functioni

    Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei332 – 3321ATPPROSITE-ProRule annotation
    Active sitei427 – 4271Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi310 – 3189ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. kinase activity Source: TAIR
    3. protein serine/threonine kinase activity Source: UniProtKB-KW

    GO - Biological processi

    1. plant-type cell wall organization Source: TAIR
    2. unidimensional cell growth Source: TAIR

    Keywords - Molecular functioni

    Kinase, Receptor, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT2G35620-MONOMER.
    ARA:GQT-1659-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    LRR receptor-like serine/threonine-protein kinase FEI 2 (EC:2.7.11.1)
    Gene namesi
    Name:FEI2
    Ordered Locus Names:At2g35620
    ORF Names:T20F21.18
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 2

    Organism-specific databases

    TAIRiAT2G35620.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: TAIR

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi336 – 3361K → R: Normal interaction with ACC synthases. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2828Sequence AnalysisAdd
    BLAST
    Chaini29 – 589561LRR receptor-like serine/threonine-protein kinase FEI 2PRO_0000387512Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi119 – 1191N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi143 – 1431N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi175 – 1751N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi215 – 2151N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi219 – 2191N-linked (GlcNAc...)Sequence Analysis
    Modified residuei384 – 3841PhosphoserineBy similarity
    Modified residuei460 – 4601PhosphothreonineBy similarity
    Modified residuei461 – 4611PhosphothreonineBy similarity
    Modified residuei466 – 4661PhosphothreonineBy similarity
    Modified residuei474 – 4741PhosphotyrosineBy similarity

    Post-translational modificationi

    Autophosphorylated.By similarity

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PaxDbiC0LGL9.
    PRIDEiC0LGL9.

    Expressioni

    Tissue specificityi

    Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

    Interactioni

    Subunit structurei

    Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT2G35620.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliC0LGL9.
    SMRiC0LGL9. Positions 32-207, 260-575.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini29 – 236208ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini258 – 589332CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei237 – 25721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati72 – 9625LRR 1Add
    BLAST
    Repeati97 – 12024LRR 2Add
    BLAST
    Repeati122 – 14423LRR 3Add
    BLAST
    Repeati145 – 16824LRR 4Add
    BLAST
    Repeati170 – 19324LRR 5Add
    BLAST
    Domaini304 – 576273Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 5 LRR (leucine-rich) repeats.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000116554.
    InParanoidiQ9ZQN5.
    OMAiFNIVGWL.
    PhylomeDBiC0LGL9.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR001611. Leu-rich_rpt.
    IPR013210. LRR-contain_N2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF13855. LRR_8. 1 hit.
    PF08263. LRRNT_2. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    C0LGL9-1 [UniParc]FASTAAdd to Basket

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    MGICLMKRCC SWFLLISFLS ALTNENEAIS PDGEALLSFR NGVLASDGVI    50
    GLWRPEDPDP CNWKGVTCDA KTKRVIALSL TYHKLRGPLP PELGKLDQLR 100
    LLMLHNNALY QSIPASLGNC TALEGIYLQN NYITGTIPSE IGNLSGLKNL 150
    DLSNNNLNGA IPASLGQLKR LTKFNVSNNF LVGKIPSDGL LARLSRDSFN 200
    GNRNLCGKQI DIVCNDSGNS TASGSPTGQG GNNPKRLLIS ASATVGGLLL 250
    VALMCFWGCF LYKKLGRVES KSLVIDVGGG ASIVMFHGDL PYASKDIIKK 300
    LESLNEEHII GCGGFGTVYK LSMDDGNVFA LKRIVKLNEG FDRFFERELE 350
    ILGSIKHRYL VNLRGYCNSP TSKLLLYDYL PGGSLDEALH KRGEQLDWDS 400
    RVNIIIGAAK GLAYLHHDCS PRIIHRDIKS SNILLDGNLE ARVSDFGLAK 450
    LLEDEESHIT TIVAGTFGYL APEYMQSGRA TEKTDVYSFG VLVLEVLSGK 500
    LPTDASFIEK GFNIVGWLNF LISENRAKEI VDLSCEGVER ESLDALLSIA 550
    TKCVSSSPDE RPTMHRVVQL LESEVMTPCP SDFYDSSSD 589
    Length:589
    Mass (Da):64,355
    Last modified:May 5, 2009 - v1
    Checksum:iA3A78CA54E1E1400
    GO

    Sequence cautioni

    The sequence AAD15451.1 differs from that shown. Reason: Erroneous gene model prediction.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006068 Genomic DNA. Translation: AAD15451.1. Sequence problems.
    CP002685 Genomic DNA. Translation: AEC09131.1.
    CP002685 Genomic DNA. Translation: AEC09132.1.
    FJ708709 mRNA. Translation: ACN59304.1.
    AK230094 mRNA. Translation: BAF01913.1.
    PIRiH84770.
    RefSeqiNP_001189684.1. NM_001202755.1.
    NP_181105.2. NM_129116.4.
    UniGeneiAt.37644.

    Genome annotation databases

    EnsemblPlantsiAT2G35620.1; AT2G35620.1; AT2G35620.
    AT2G35620.2; AT2G35620.2; AT2G35620.
    GeneIDi818130.
    KEGGiath:AT2G35620.

    Cross-referencesi

    Web resourcesi

    PlantP kinase Classification PPC

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC006068 Genomic DNA. Translation: AAD15451.1 . Sequence problems.
    CP002685 Genomic DNA. Translation: AEC09131.1 .
    CP002685 Genomic DNA. Translation: AEC09132.1 .
    FJ708709 mRNA. Translation: ACN59304.1 .
    AK230094 mRNA. Translation: BAF01913.1 .
    PIRi H84770.
    RefSeqi NP_001189684.1. NM_001202755.1.
    NP_181105.2. NM_129116.4.
    UniGenei At.37644.

    3D structure databases

    ProteinModelPortali C0LGL9.
    SMRi C0LGL9. Positions 32-207, 260-575.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT2G35620.1-P.

    Proteomic databases

    PaxDbi C0LGL9.
    PRIDEi C0LGL9.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT2G35620.1 ; AT2G35620.1 ; AT2G35620 .
    AT2G35620.2 ; AT2G35620.2 ; AT2G35620 .
    GeneIDi 818130.
    KEGGi ath:AT2G35620.

    Organism-specific databases

    GeneFarmi 2548. 221.
    TAIRi AT2G35620.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000116554.
    InParanoidi Q9ZQN5.
    OMAi FNIVGWL.
    PhylomeDBi C0LGL9.

    Enzyme and pathway databases

    BioCyci ARA:AT2G35620-MONOMER.
    ARA:GQT-1659-MONOMER.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR001611. Leu-rich_rpt.
    IPR013210. LRR-contain_N2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF13855. LRR_8. 1 hit.
    PF08263. LRRNT_2. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
      Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
      BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs."
      Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A., Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y., Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.
      , Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y., Shinozaki K.
      Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 294-589.
      Strain: cv. Columbia.
    5. "Two leucine-rich repeat receptor kinases mediate signaling, linking cell wall biosynthesis and ACC synthase in Arabidopsis."
      Xu S.-L., Rahman A., Baskin T.I., Kieber J.J.
      Plant Cell 20:3065-3079(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LYS-336, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH ACS5 AND ACS9.

    Entry informationi

    Entry nameiFEI2_ARATH
    AccessioniPrimary (citable) accession number: C0LGL9
    Secondary accession number(s): Q0WLU4, Q9ZQN5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 44 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    'Fei' means fat in Chinese.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3