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Protein

LRR receptor-like serine/threonine-protein kinase FEI 2

Gene

FEI2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

Miscellaneous

'Fei' means fat in Chinese.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei332ATPPROSITE-ProRule annotation1
Active sitei427Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi310 – 318ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • kinase activity Source: TAIR
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • cellulose biosynthetic process Source: InterPro
  • mucilage biosynthetic process Source: TAIR
  • mucilage biosynthetic process involved in seed coat development Source: TAIR
  • plant-type cell wall organization Source: TAIR
  • unidimensional cell growth Source: TAIR

Keywordsi

Molecular functionKinase, Receptor, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase FEI 2 (EC:2.7.11.1)
Gene namesi
Name:FEI2
Ordered Locus Names:At2g35620
ORF Names:T20F21.18
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

AraportiAT2G35620
TAIRilocus:2058759 AT2G35620

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini29 – 236ExtracellularSequence analysisAdd BLAST208
Transmembranei237 – 257HelicalSequence analysisAdd BLAST21
Topological domaini258 – 589CytoplasmicSequence analysisAdd BLAST332

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi336K → R: Normal interaction with ACC synthases. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 28Sequence analysisAdd BLAST28
ChainiPRO_000038751229 – 589LRR receptor-like serine/threonine-protein kinase FEI 2Add BLAST561

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi119N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi143N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi175N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi215N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi219N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei384PhosphoserineBy similarity1
Modified residuei460PhosphothreonineBy similarity1
Modified residuei461PhosphothreonineBy similarity1
Modified residuei466PhosphothreonineBy similarity1
Modified residuei474PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiC0LGL9
PRIDEiC0LGL9

Expressioni

Tissue specificityi

Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

Gene expression databases

ExpressionAtlasiC0LGL9 baseline and differential
GenevisibleiC0LGL9 AT

Interactioni

Subunit structurei

Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

Protein-protein interaction databases

BioGridi3475, 24 interactors
STRINGi3702.AT2G35620.1

Structurei

3D structure databases

ProteinModelPortaliC0LGL9
SMRiC0LGL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati72 – 96LRR 1Add BLAST25
Repeati97 – 120LRR 2Add BLAST24
Repeati122 – 144LRR 3Add BLAST23
Repeati145 – 168LRR 4Add BLAST24
Repeati170 – 193LRR 5Add BLAST24
Domaini304 – 576Protein kinasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIXE Eukaryota
COG0515 LUCA
HOGENOMiHOG000116554
InParanoidiC0LGL9
OMAiVTENRQR
OrthoDBiEOG093605FU
PhylomeDBiC0LGL9

Family and domain databases

Gene3Di3.80.10.10, 1 hit
InterProiView protein in InterPro
IPR031071 FEI1/2
IPR011009 Kinase-like_dom_sf
IPR001611 Leu-rich_rpt
IPR003591 Leu-rich_rpt_typical-subtyp
IPR032675 LRR_dom_sf
IPR013210 LRR_N_plant-typ
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR44289:SF1 PTHR44289:SF1, 1 hit
PfamiView protein in Pfam
PF13855 LRR_8, 1 hit
PF08263 LRRNT_2, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00369 LRR_TYP, 2 hits
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

C0LGL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGICLMKRCC SWFLLISFLS ALTNENEAIS PDGEALLSFR NGVLASDGVI
60 70 80 90 100
GLWRPEDPDP CNWKGVTCDA KTKRVIALSL TYHKLRGPLP PELGKLDQLR
110 120 130 140 150
LLMLHNNALY QSIPASLGNC TALEGIYLQN NYITGTIPSE IGNLSGLKNL
160 170 180 190 200
DLSNNNLNGA IPASLGQLKR LTKFNVSNNF LVGKIPSDGL LARLSRDSFN
210 220 230 240 250
GNRNLCGKQI DIVCNDSGNS TASGSPTGQG GNNPKRLLIS ASATVGGLLL
260 270 280 290 300
VALMCFWGCF LYKKLGRVES KSLVIDVGGG ASIVMFHGDL PYASKDIIKK
310 320 330 340 350
LESLNEEHII GCGGFGTVYK LSMDDGNVFA LKRIVKLNEG FDRFFERELE
360 370 380 390 400
ILGSIKHRYL VNLRGYCNSP TSKLLLYDYL PGGSLDEALH KRGEQLDWDS
410 420 430 440 450
RVNIIIGAAK GLAYLHHDCS PRIIHRDIKS SNILLDGNLE ARVSDFGLAK
460 470 480 490 500
LLEDEESHIT TIVAGTFGYL APEYMQSGRA TEKTDVYSFG VLVLEVLSGK
510 520 530 540 550
LPTDASFIEK GFNIVGWLNF LISENRAKEI VDLSCEGVER ESLDALLSIA
560 570 580
TKCVSSSPDE RPTMHRVVQL LESEVMTPCP SDFYDSSSD
Length:589
Mass (Da):64,355
Last modified:May 5, 2009 - v1
Checksum:iA3A78CA54E1E1400
GO

Sequence cautioni

The sequence AAD15451 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC006068 Genomic DNA Translation: AAD15451.1 Sequence problems.
CP002685 Genomic DNA Translation: AEC09131.1
CP002685 Genomic DNA Translation: AEC09132.1
CP002685 Genomic DNA Translation: ANM61923.1
CP002685 Genomic DNA Translation: ANM61924.1
FJ708709 mRNA Translation: ACN59304.1
AK230094 mRNA Translation: BAF01913.1
PIRiH84770
RefSeqiNP_001189684.1, NM_001202755.2
NP_001324112.1, NM_001336566.1
NP_001324113.1, NM_001336565.1
NP_181105.2, NM_129116.5
UniGeneiAt.37644

Genome annotation databases

EnsemblPlantsiAT2G35620.1; AT2G35620.1; AT2G35620
AT2G35620.2; AT2G35620.2; AT2G35620
AT2G35620.3; AT2G35620.3; AT2G35620
AT2G35620.4; AT2G35620.4; AT2G35620
GeneIDi818130
GrameneiAT2G35620.1; AT2G35620.1; AT2G35620
AT2G35620.2; AT2G35620.2; AT2G35620
AT2G35620.3; AT2G35620.3; AT2G35620
AT2G35620.4; AT2G35620.4; AT2G35620
KEGGiath:AT2G35620

Similar proteinsi

Entry informationi

Entry nameiFEI2_ARATH
AccessioniPrimary (citable) accession number: C0LGL9
Secondary accession number(s): Q0WLU4, Q9ZQN5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: April 25, 2018
This is version 71 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health