C0LGH8 (Y1634_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable LRR receptor-like serine/threonine-protein kinase At1g63430
EC=2.7.11.1

Gene names

Ordered Locus Names:	At1g63430
ORF Names:	F2K11.19

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	664 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subcellular location	Cell membrane; Single-pass type I membrane protein.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 4 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence AAF19706.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: C0LGH8-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 664	642	Probable LRR receptor-like serine/threonine-protein kinase At1g63430		PRO_0000387538
Regions
	Topological domain	23 – 288	266	Extracellular Potential
	Transmembrane	289 – 309	21	Helical; Potential
	Topological domain	310 – 664	355	Cytoplasmic Potential
	Repeat	94 – 116	23	LRR 1
	Repeat	118 – 140	23	LRR 2
	Repeat	142 – 165	24	LRR 3
	Repeat	166 – 178	13	LRR 4
	Domain	360 – 642	283	Protein kinase
Amino acid modifications
	Glycosylation	75	1	N-linked (GlcNAc...) Potential
	Glycosylation	197	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	50	1	N → D in BAC42570. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 8520F165DA7CB366	FASTA	664	73,910
	10 20 30 40 50 60 MRSKYFCSLA LVLGLFFVSC DGFASNEVQA LRRFKEAIYE DPLLVMSNWN DPNSDPCDWT 70 80 90 100 110 120 GIYCSPSKDH VIKINISASS IKGFLAPELG QITYLQELIL HGNILIGTIP KEIGNLKNLK 130 140 150 160 170 180 ILDLGNNHLM GPIPAEIGSL SGIMIINLQS NGLTGKLPAE LGNLKYLREL HIDRNRLQGS 190 200 210 220 230 240 LLVAGASGYQ SKVYSSNSSA NIAGLCKSLK VADFSYNFFV GNIPKCLENL PRTSFQGNCM 250 260 270 280 290 300 QNKDLKHRSS SQCANAQLVK THGSPSAAPK HQSAQMVAKH HRASKPKWLL ALEIVTGSMV 310 320 330 340 350 360 GLLLLVALFS AVHRWNNRST LIIPWKKSSS EKEKFTVYVD SEMLKDVSRL TRQELEVACE 370 380 390 400 410 420 DFSNIIGLSA DSQIYKGTLK GGSEIAVISL CVKEEDWTGY LELYFQREVA DLARLNHENT 430 440 450 460 470 480 AKLLGYCKEI SPFTRMLVFE YASNGTLYEH LHYGEAALVS WARRMKIVIG IARGLKYLHM 490 500 510 520 530 540 ELDPPFTISE LSSNAIYLTE DFTPKLVDFE CWKTILARSE KNLRNISSQG SICVLPNGME 550 560 570 580 590 600 SRYLDVSGNI YAFGILLLEI VSGRPPYCKD KGFLIEWAKE FLEAPEAMSG LVDPELKHFN 610 620 630 640 650 660 QEDLETVCEV ASQCLNRDPT NNNNNHNKPS VQELCETLES RISLSISAEL RSSSLAWAEL ALDS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Functional annotation of a full-length Arabidopsis cDNA collection." Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K. Science 296:141-145(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[5]	"Quantitative phosphoproteomics of early elicitor signaling in Arabidopsis." Benschop J.J., Mohammed S., O'Flaherty M., Heck A.J.R., Slijper M., Menke F.L.H. Mol. Cell. Proteomics 6:1198-1214(2007) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], SUBCELLULAR LOCATION. Strain: cv. Columbia.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC008047 Genomic DNA. Translation: AAF19706.1. Sequence problems. CP002684 Genomic DNA. Translation: AEE34098.1. AK117932 mRNA. Translation: BAC42570.1. FJ708668 mRNA. Translation: ACN59263.1.
IPI	IPI00543834.
RefSeq	NP_176532.2. NM_105022.2.
UniGene	At.14999.
3D structure databases
ProteinModelPortal	C0LGH8.
SMR	C0LGH8. Positions 55-227, 366-638.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G63430.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G63430.1; AT1G63430.1; AT1G63430.
GeneID	842649.
KEGG	ath:AT1G63430.
Organism-specific databases
GeneFarm	693. 50.
TAIR	At1g63430.
Phylogenomic databases
eggNOG	NOG305556.
HOGENOM	HOG000240929.
InParanoid	Q8GY10.
PhylomeDB	C0LGH8.
ProtClustDB	CLSN2686223.
Gene expression databases
ArrayExpress	C0LGH8.
Family and domain databases
Gene3D	2.60.120.200. 1 hit.
InterPro	IPR013320. ConA-like_subgrp. IPR011009. Kinase-like_dom. IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. PF08263. LRRNT_2. 1 hit. PF07714. Pkinase_Tyr. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. False negative. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y1634_ARATH

Accession

Primary (citable) accession number: C0LGH8
Secondary accession number(s): Q8GY10, Q9SH29

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program