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C0LGF4

- FEI1_ARATH

UniProt

C0LGF4 - FEI1_ARATH

Protein

LRR receptor-like serine/threonine-protein kinase FEI 1

Gene

FEI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 50 (01 Oct 2014)
      Sequence version 1 (05 May 2009)
      Previous versions | rss
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    Functioni

    Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei334 – 3341ATPPROSITE-ProRule annotation
    Active sitei429 – 4291Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi312 – 3209ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. protein serine/threonine kinase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Receptor, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G31420-MONOMER.
    ARA:GQT-1631-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    LRR receptor-like serine/threonine-protein kinase FEI 1 (EC:2.7.11.1)
    Gene namesi
    Name:FEI1
    Ordered Locus Names:At1g31420
    ORF Names:T8E3.2
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G31420.

    Subcellular locationi

    Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi334 – 3341K → R: Loss of kinase activity, normal interaction with ACC synthases. 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 591562LRR receptor-like serine/threonine-protein kinase FEI 1PRO_0000387511Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi80 – 801N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi176 – 1761N-linked (GlcNAc...)Sequence Analysis
    Modified residuei386 – 3861PhosphoserineBy similarity
    Modified residuei462 – 4621PhosphothreonineBy similarity
    Modified residuei463 – 4631PhosphothreonineBy similarity
    Modified residuei468 – 4681PhosphothreonineBy similarity
    Modified residuei476 – 4761PhosphotyrosineBy similarity

    Post-translational modificationi

    Autophosphorylated.

    Keywords - PTMi

    Glycoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiC0LGF4.

    Expressioni

    Tissue specificityi

    Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

    Gene expression databases

    ArrayExpressiC0LGF4.

    Interactioni

    Subunit structurei

    Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

    Protein-protein interaction databases

    STRINGi3702.AT1G31420.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliC0LGF4.
    SMRiC0LGF4. Positions 33-208, 263-575.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini30 – 238209ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini260 – 591332CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei239 – 25921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati73 – 9725LRR 1Add
    BLAST
    Repeati98 – 12124LRR 2Add
    BLAST
    Repeati123 – 14523LRR 3Add
    BLAST
    Repeati146 – 16924LRR 4Add
    BLAST
    Repeati171 – 19424LRR 5Add
    BLAST
    Domaini306 – 578273Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
    Contains 5 LRR (leucine-rich) repeats.Curated
    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000116554.
    InParanoidiQ9C867.
    PhylomeDBiC0LGF4.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR001611. Leu-rich_rpt.
    IPR013210. LRR-contain_N2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PfamiPF00560. LRR_1. 1 hit.
    PF08263. LRRNT_2. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced. According to EST sequences.

    Isoform 1 (identifier: C0LGF4-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MMGICEMKSC CSWLLLISLL CSLSNESQAI SPDGEALLSF RNAVTRSDSF    50
    IHQWRPEDPD PCNWNGVTCD AKTKRVITLN LTYHKIMGPL PPDIGKLDHL 100
    RLLMLHNNAL YGAIPTALGN CTALEEIHLQ SNYFTGPIPA EMGDLPGLQK 150
    LDMSSNTLSG PIPASLGQLK KLSNFNVSNN FLVGQIPSDG VLSGFSKNSF 200
    IGNLNLCGKH VDVVCQDDSG NPSSHSQSGQ NQKKNSGKLL ISASATVGAL 250
    LLVALMCFWG CFLYKKLGKV EIKSLAKDVG GGASIVMFHG DLPYSSKDII 300
    KKLEMLNEEH IIGCGGFGTV YKLAMDDGKV FALKRILKLN EGFDRFFERE 350
    LEILGSIKHR YLVNLRGYCN SPTSKLLLYD YLPGGSLDEA LHERGEQLDW 400
    DSRVNIIIGA AKGLSYLHHD CSPRIIHRDI KSSNILLDGN LEARVSDFGL 450
    AKLLEDEESH ITTIVAGTFG YLAPEYMQSG RATEKTDVYS FGVLVLEVLS 500
    GKRPTDASFI EKGLNVVGWL KFLISEKRPR DIVDPNCEGM QMESLDALLS 550
    IATQCVSPSP EERPTMHRVV QLLESEVMTP CPSEFYDSSS D 591
    Length:591
    Mass (Da):64,916
    Last modified:May 5, 2009 - v1
    Checksum:iEA57944B55157D91
    GO

    Sequence cautioni

    The sequence AAG51266.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC027135 Genomic DNA. Translation: AAG51266.1. Different initiation.
    CP002684 Genomic DNA. Translation: AEE31354.1.
    FJ708643 mRNA. Translation: ACN59239.1.
    PIRiB86440.
    RefSeqiNP_001185122.1. NM_001198193.1. [C0LGF4-1]
    UniGeneiAt.27683.
    At.67250.

    Genome annotation databases

    EnsemblPlantsiAT1G31420.2; AT1G31420.2; AT1G31420. [C0LGF4-1]
    GeneIDi840032.
    KEGGiath:AT1G31420.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Web resourcesi

    PlantP kinase Classification PPC

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AC027135 Genomic DNA. Translation: AAG51266.1 . Different initiation.
    CP002684 Genomic DNA. Translation: AEE31354.1 .
    FJ708643 mRNA. Translation: ACN59239.1 .
    PIRi B86440.
    RefSeqi NP_001185122.1. NM_001198193.1. [C0LGF4-1 ]
    UniGenei At.27683.
    At.67250.

    3D structure databases

    ProteinModelPortali C0LGF4.
    SMRi C0LGF4. Positions 33-208, 263-575.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT1G31420.1-P.

    Proteomic databases

    PRIDEi C0LGF4.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G31420.2 ; AT1G31420.2 ; AT1G31420 . [C0LGF4-1 ]
    GeneIDi 840032.
    KEGGi ath:AT1G31420.

    Organism-specific databases

    GeneFarmi 2555. 221.
    TAIRi AT1G31420.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000116554.
    InParanoidi Q9C867.
    PhylomeDBi C0LGF4.

    Enzyme and pathway databases

    BioCyci ARA:AT1G31420-MONOMER.
    ARA:GQT-1631-MONOMER.

    Gene expression databases

    ArrayExpressi C0LGF4.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR001611. Leu-rich_rpt.
    IPR013210. LRR-contain_N2.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    Pfami PF00560. LRR_1. 1 hit.
    PF08263. LRRNT_2. 1 hit.
    PF00069. Pkinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    2. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    3. "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
      Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
      BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.
    4. "Two leucine-rich repeat receptor kinases mediate signaling, linking cell wall biosynthesis and ACC synthase in Arabidopsis."
      Xu S.-L., Rahman A., Baskin T.I., Kieber J.J.
      Plant Cell 20:3065-3079(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, MUTAGENESIS OF LYS-334, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH ACS5 AND ACS9.

    Entry informationi

    Entry nameiFEI1_ARATH
    AccessioniPrimary (citable) accession number: C0LGF4
    Secondary accession number(s): Q9C867
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 3, 2009
    Last sequence update: May 5, 2009
    Last modified: October 1, 2014
    This is version 50 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    'Fei' means fat in Chinese.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3