C0LGF4 (FEI1_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
LRR receptor-like serine/threonine-protein kinase FEI 1
EC=2.7.11.1

Gene names

Name:	FEI1
Ordered Locus Names:	At1g31420
ORF Names:	T8E3.2

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	591 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene). Ref.4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein.
Subunit structure	Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain. Ref.4
Subcellular location	Cell membrane; Single-pass type I membrane protein Ref.4.
Tissue specificity	Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings. Ref.4
Post-translational modification	Autophosphorylated.
Disruption phenotype	No visible phenotype. Double mutants fei1-fei2 exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis. Ref.4
Miscellaneous	'Fei' means fat in Chinese.
Sequence similarities	Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Contains 5 LRR (leucine-rich) repeats. Contains 1 protein kinase domain.
Sequence caution	The sequence AAG51266.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cell membrane Membrane
Coding sequence diversity	Alternative splicing
Domain	Leucine-rich repeat Repeat Signal Transmembrane Transmembrane helix
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Receptor Serine/threonine-protein kinase Transferase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW protein serine/threonine kinase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: C0LGF4-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 591	562	LRR receptor-like serine/threonine-protein kinase FEI 1		PRO_0000387511
Regions
	Topological domain	30 – 238	209	Extracellular Potential
	Transmembrane	239 – 259	21	Helical; Potential
	Topological domain	260 – 591	332	Cytoplasmic Potential
	Repeat	73 – 97	25	LRR 1
	Repeat	98 – 121	24	LRR 2
	Repeat	123 – 145	23	LRR 3
	Repeat	146 – 169	24	LRR 4
	Repeat	171 – 194	24	LRR 5
	Domain	306 – 578	273	Protein kinase
	Nucleotide binding	312 – 320	9	ATP By similarity
Sites
	Active site	429	1	Proton acceptor By similarity
	Binding site	334	1	ATP By similarity
Amino acid modifications
	Glycosylation	80	1	N-linked (GlcNAc...) Potential
	Glycosylation	120	1	N-linked (GlcNAc...) Potential
	Glycosylation	176	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	334	1	K → R: Loss of kinase activity, normal interaction with ACC synthases. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: EA57944B55157D91	FASTA	591	64,916
	10 20 30 40 50 60 MMGICEMKSC CSWLLLISLL CSLSNESQAI SPDGEALLSF RNAVTRSDSF IHQWRPEDPD 70 80 90 100 110 120 PCNWNGVTCD AKTKRVITLN LTYHKIMGPL PPDIGKLDHL RLLMLHNNAL YGAIPTALGN 130 140 150 160 170 180 CTALEEIHLQ SNYFTGPIPA EMGDLPGLQK LDMSSNTLSG PIPASLGQLK KLSNFNVSNN 190 200 210 220 230 240 FLVGQIPSDG VLSGFSKNSF IGNLNLCGKH VDVVCQDDSG NPSSHSQSGQ NQKKNSGKLL 250 260 270 280 290 300 ISASATVGAL LLVALMCFWG CFLYKKLGKV EIKSLAKDVG GGASIVMFHG DLPYSSKDII 310 320 330 340 350 360 KKLEMLNEEH IIGCGGFGTV YKLAMDDGKV FALKRILKLN EGFDRFFERE LEILGSIKHR 370 380 390 400 410 420 YLVNLRGYCN SPTSKLLLYD YLPGGSLDEA LHERGEQLDW DSRVNIIIGA AKGLSYLHHD 430 440 450 460 470 480 CSPRIIHRDI KSSNILLDGN LEARVSDFGL AKLLEDEESH ITTIVAGTFG YLAPEYMQSG 490 500 510 520 530 540 RATEKTDVYS FGVLVLEVLS GKRPTDASFI EKGLNVVGWL KFLISEKRPR DIVDPNCEGM 550 560 570 580 590 QMESLDALLS IATQCVSPSP EERPTMHRVV QLLESEVMTP CPSEFYDSSS D

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[2]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[3]	"Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana." Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J. BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
[4]	"Two leucine-rich repeat receptor kinases mediate signaling, linking cell wall biosynthesis and ACC synthase in Arabidopsis." Xu S.-L., Rahman A., Baskin T.I., Kieber J.J. Plant Cell 20:3065-3079(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, MUTAGENESIS OF LYS-334, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH ACS5 AND ACS9.

Web resources

PlantP kinase Classification PPC

Cross-references

Sequence databases
EMBL GenBank DDBJ	AC027135 Genomic DNA. Translation: AAG51266.1. Different initiation. CP002684 Genomic DNA. Translation: AEE31354.1. FJ708643 mRNA. Translation: ACN59239.1.
IPI	IPI00991547.
PIR	B86440.
RefSeq	NP_001185122.1. NM_001198193.1.
UniGene	At.27683. At.67250.
3D structure databases
ProteinModelPortal	C0LGF4.
SMR	C0LGF4. Positions 33-208, 263-576.
ModBase	Search...
Protein-protein interaction databases
STRING	3702.AT1G31420.1-P.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G31420.2; AT1G31420.2; AT1G31420.
GeneID	840032.
KEGG	ath:AT1G31420.
Organism-specific databases
GeneFarm	2555. 221.
TAIR	At1g31420.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000116554.
InParanoid	Q9C867.
PhylomeDB	C0LGF4.
Gene expression databases
ArrayExpress	C0LGF4.
Family and domain databases
InterPro	IPR011009. Kinase-like_dom. IPR001611. Leu-rich_rpt. IPR013210. LRR-contain_N2. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
Pfam	PF00560. LRR_1. 1 hit. PF08263. LRRNT_2. 1 hit. PF00069. Pkinase. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FEI1_ARATH

Accession

Primary (citable) accession number: C0LGF4
Secondary accession number(s): Q9C867

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 3, 2009
Last sequence update:	May 5, 2009
Last modified:	May 1, 2013
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program