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C0LGF4

- FEI1_ARATH

UniProt

C0LGF4 - FEI1_ARATH

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Protein
LRR receptor-like serine/threonine-protein kinase FEI 1
Gene
FEI1, At1g31420, T8E3.2
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei334 – 3341ATP By similarity
Active sitei429 – 4291Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi312 – 3209ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G31420-MONOMER.
ARA:GQT-1631-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase FEI 1 (EC:2.7.11.1)
Gene namesi
Name:FEI1
Ordered Locus Names:At1g31420
ORF Names:T8E3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G31420.

Subcellular locationi

Cell membrane; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini30 – 238209Extracellular Reviewed prediction
Add
BLAST
Transmembranei239 – 25921Helical; Reviewed prediction
Add
BLAST
Topological domaini260 – 591332Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi334 – 3341K → R: Loss of kinase activity, normal interaction with ACC synthases. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2929 Reviewed prediction
Add
BLAST
Chaini30 – 591562LRR receptor-like serine/threonine-protein kinase FEI 1
PRO_0000387511Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi80 – 801N-linked (GlcNAc...) Reviewed prediction
Glycosylationi120 – 1201N-linked (GlcNAc...) Reviewed prediction
Glycosylationi176 – 1761N-linked (GlcNAc...) Reviewed prediction
Modified residuei386 – 3861Phosphoserine By similarity
Modified residuei462 – 4621Phosphothreonine By similarity
Modified residuei463 – 4631Phosphothreonine By similarity
Modified residuei468 – 4681Phosphothreonine By similarity
Modified residuei476 – 4761Phosphotyrosine By similarity

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiC0LGF4.

Expressioni

Tissue specificityi

Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

Gene expression databases

ArrayExpressiC0LGF4.

Interactioni

Subunit structurei

Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

Protein-protein interaction databases

STRINGi3702.AT1G31420.1-P.

Structurei

3D structure databases

ProteinModelPortaliC0LGF4.
SMRiC0LGF4. Positions 33-208, 263-575.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati73 – 9725LRR 1
Add
BLAST
Repeati98 – 12124LRR 2
Add
BLAST
Repeati123 – 14523LRR 3
Add
BLAST
Repeati146 – 16924LRR 4
Add
BLAST
Repeati171 – 19424LRR 5
Add
BLAST
Domaini306 – 578273Protein kinase
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000116554.
InParanoidiQ9C867.
PhylomeDBiC0LGF4.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00560. LRR_1. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced. According to EST sequences.

Isoform 1 (identifier: C0LGF4-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MMGICEMKSC CSWLLLISLL CSLSNESQAI SPDGEALLSF RNAVTRSDSF    50
IHQWRPEDPD PCNWNGVTCD AKTKRVITLN LTYHKIMGPL PPDIGKLDHL 100
RLLMLHNNAL YGAIPTALGN CTALEEIHLQ SNYFTGPIPA EMGDLPGLQK 150
LDMSSNTLSG PIPASLGQLK KLSNFNVSNN FLVGQIPSDG VLSGFSKNSF 200
IGNLNLCGKH VDVVCQDDSG NPSSHSQSGQ NQKKNSGKLL ISASATVGAL 250
LLVALMCFWG CFLYKKLGKV EIKSLAKDVG GGASIVMFHG DLPYSSKDII 300
KKLEMLNEEH IIGCGGFGTV YKLAMDDGKV FALKRILKLN EGFDRFFERE 350
LEILGSIKHR YLVNLRGYCN SPTSKLLLYD YLPGGSLDEA LHERGEQLDW 400
DSRVNIIIGA AKGLSYLHHD CSPRIIHRDI KSSNILLDGN LEARVSDFGL 450
AKLLEDEESH ITTIVAGTFG YLAPEYMQSG RATEKTDVYS FGVLVLEVLS 500
GKRPTDASFI EKGLNVVGWL KFLISEKRPR DIVDPNCEGM QMESLDALLS 550
IATQCVSPSP EERPTMHRVV QLLESEVMTP CPSEFYDSSS D 591
Length:591
Mass (Da):64,916
Last modified:May 5, 2009 - v1
Checksum:iEA57944B55157D91
GO

Sequence cautioni

The sequence AAG51266.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC027135 Genomic DNA. Translation: AAG51266.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE31354.1.
FJ708643 mRNA. Translation: ACN59239.1.
PIRiB86440.
RefSeqiNP_001185122.1. NM_001198193.1. [C0LGF4-1]
UniGeneiAt.27683.
At.67250.

Genome annotation databases

EnsemblPlantsiAT1G31420.2; AT1G31420.2; AT1G31420. [C0LGF4-1]
GeneIDi840032.
KEGGiath:AT1G31420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AC027135 Genomic DNA. Translation: AAG51266.1 . Different initiation.
CP002684 Genomic DNA. Translation: AEE31354.1 .
FJ708643 mRNA. Translation: ACN59239.1 .
PIRi B86440.
RefSeqi NP_001185122.1. NM_001198193.1. [C0LGF4-1 ]
UniGenei At.27683.
At.67250.

3D structure databases

ProteinModelPortali C0LGF4.
SMRi C0LGF4. Positions 33-208, 263-575.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT1G31420.1-P.

Proteomic databases

PRIDEi C0LGF4.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G31420.2 ; AT1G31420.2 ; AT1G31420 . [C0LGF4-1 ]
GeneIDi 840032.
KEGGi ath:AT1G31420.

Organism-specific databases

GeneFarmi 2555. 221.
TAIRi AT1G31420.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000116554.
InParanoidi Q9C867.
PhylomeDBi C0LGF4.

Enzyme and pathway databases

BioCyci ARA:AT1G31420-MONOMER.
ARA:GQT-1631-MONOMER.

Gene expression databases

ArrayExpressi C0LGF4.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR001611. Leu-rich_rpt.
IPR013210. LRR-contain_N2.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00560. LRR_1. 1 hit.
PF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  2. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  3. "Genome-wide cloning and sequence analysis of leucine-rich repeat receptor-like protein kinase genes in Arabidopsis thaliana."
    Gou X., He K., Yang H., Yuan T., Lin H., Clouse S.D., Li J.
    BMC Genomics 11:19-19(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.
  4. "Two leucine-rich repeat receptor kinases mediate signaling, linking cell wall biosynthesis and ACC synthase in Arabidopsis."
    Xu S.-L., Rahman A., Baskin T.I., Kieber J.J.
    Plant Cell 20:3065-3079(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, MUTAGENESIS OF LYS-334, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE, INTERACTION WITH ACS5 AND ACS9.

Entry informationi

Entry nameiFEI1_ARATH
AccessioniPrimary (citable) accession number: C0LGF4
Secondary accession number(s): Q9C867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: June 11, 2014
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'Fei' means fat in Chinese.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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