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Protein

LRR receptor-like serine/threonine-protein kinase FEI 1

Gene

FEI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

Miscellaneous

'Fei' means fat in Chinese.

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei334ATPPROSITE-ProRule annotation1
Active sitei429Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi312 – 320ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Receptor, Serine/threonine-protein kinase, Transferase
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase FEI 1 (EC:2.7.11.1)
Gene namesi
Name:FEI1
Ordered Locus Names:At1g31420
ORF Names:T8E3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G31420

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 238ExtracellularSequence analysisAdd BLAST209
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 591CytoplasmicSequence analysisAdd BLAST332

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi334K → R: Loss of kinase activity, normal interaction with ACC synthases. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000038751130 – 591LRR receptor-like serine/threonine-protein kinase FEI 1Add BLAST562

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi120N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi176N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei386PhosphoserineBy similarity1
Modified residuei462PhosphothreonineBy similarity1
Modified residuei463PhosphothreonineBy similarity1
Modified residuei468PhosphothreonineBy similarity1
Modified residuei476PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiC0LGF4

Expressioni

Tissue specificityi

Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

Gene expression databases

ExpressionAtlasiC0LGF4 baseline and differential
GenevisibleiC0LGF4 AT

Interactioni

Subunit structurei

Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

Protein-protein interaction databases

BioGridi2526735 interactors.
STRINGi3702.AT1G31420.1

Structurei

3D structure databases

ProteinModelPortaliC0LGF4
SMRiC0LGF4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati73 – 97LRR 1Add BLAST25
Repeati98 – 121LRR 2Add BLAST24
Repeati123 – 145LRR 3Add BLAST23
Repeati146 – 169LRR 4Add BLAST24
Repeati171 – 194LRR 5Add BLAST24
Domaini306 – 578Protein kinasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIXE Eukaryota
COG0515 LUCA
HOGENOMiHOG000116554
InParanoidiC0LGF4
PhylomeDBiC0LGF4

Family and domain databases

Gene3Di3.80.10.101 hit
InterProiView protein in InterPro
IPR031071 FEI1/2
IPR011009 Kinase-like_dom_sf
IPR032675 LRR_dom_sf
IPR013210 LRR_N_plant-typ
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PANTHERiPTHR44289:SF1 PTHR44289:SF1, 1 hit
PfamiView protein in Pfam
PF08263 LRRNT_2, 1 hit
PF00069 Pkinase, 1 hit
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: C0LGF4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMGICEMKSC CSWLLLISLL CSLSNESQAI SPDGEALLSF RNAVTRSDSF
60 70 80 90 100
IHQWRPEDPD PCNWNGVTCD AKTKRVITLN LTYHKIMGPL PPDIGKLDHL
110 120 130 140 150
RLLMLHNNAL YGAIPTALGN CTALEEIHLQ SNYFTGPIPA EMGDLPGLQK
160 170 180 190 200
LDMSSNTLSG PIPASLGQLK KLSNFNVSNN FLVGQIPSDG VLSGFSKNSF
210 220 230 240 250
IGNLNLCGKH VDVVCQDDSG NPSSHSQSGQ NQKKNSGKLL ISASATVGAL
260 270 280 290 300
LLVALMCFWG CFLYKKLGKV EIKSLAKDVG GGASIVMFHG DLPYSSKDII
310 320 330 340 350
KKLEMLNEEH IIGCGGFGTV YKLAMDDGKV FALKRILKLN EGFDRFFERE
360 370 380 390 400
LEILGSIKHR YLVNLRGYCN SPTSKLLLYD YLPGGSLDEA LHERGEQLDW
410 420 430 440 450
DSRVNIIIGA AKGLSYLHHD CSPRIIHRDI KSSNILLDGN LEARVSDFGL
460 470 480 490 500
AKLLEDEESH ITTIVAGTFG YLAPEYMQSG RATEKTDVYS FGVLVLEVLS
510 520 530 540 550
GKRPTDASFI EKGLNVVGWL KFLISEKRPR DIVDPNCEGM QMESLDALLS
560 570 580 590
IATQCVSPSP EERPTMHRVV QLLESEVMTP CPSEFYDSSS D
Length:591
Mass (Da):64,916
Last modified:May 5, 2009 - v1
Checksum:iEA57944B55157D91
GO

Sequence cautioni

The sequence AAG51266 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC027135 Genomic DNA Translation: AAG51266.1 Different initiation.
CP002684 Genomic DNA Translation: AEE31354.1
FJ708643 mRNA Translation: ACN59239.1
PIRiB86440
RefSeqiNP_001185122.1, NM_001198193.1 [C0LGF4-1]
UniGeneiAt.27683
At.67250

Genome annotation databases

EnsemblPlantsiAT1G31420.2; AT1G31420.2; AT1G31420 [C0LGF4-1]
GeneIDi840032
GrameneiAT1G31420.2; AT1G31420.2; AT1G31420 [C0LGF4-1]
KEGGiath:AT1G31420

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiFEI1_ARATH
AccessioniPrimary (citable) accession number: C0LGF4
Secondary accession number(s): Q9C867
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: April 25, 2018
This is version 73 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome