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Protein

LRR receptor-like serine/threonine-protein kinase FEI 1

Gene

FEI1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the signaling pathway that regulates cell wall function, including cellulose biosynthesis, likely via an 1-aminocyclopropane-1-carboxylic acid (ACC)-mediated signal (a precursor of ethylene).1 Publication

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei334ATPPROSITE-ProRule annotation1
Active sitei429Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi312 – 320ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Receptor, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
LRR receptor-like serine/threonine-protein kinase FEI 1 (EC:2.7.11.1)
Gene namesi
Name:FEI1
Ordered Locus Names:At1g31420
ORF Names:T8E3.2
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G31420.

Subcellular locationi

  • Cell membrane 1 Publication; Single-pass type I membrane protein 1 Publication

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini30 – 238ExtracellularSequence analysisAdd BLAST209
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 591CytoplasmicSequence analysisAdd BLAST332

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Fei1 and fei2 double mutants exhibit disrupted anisotropic expansion (e.g. during hypocotyl elongation), impaired synthesis of cell wall polymers, and abnormal cellulose biosynthesis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi334K → R: Loss of kinase activity, normal interaction with ACC synthases. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000038751130 – 591LRR receptor-like serine/threonine-protein kinase FEI 1Add BLAST562

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...)Sequence analysis1
Glycosylationi120N-linked (GlcNAc...)Sequence analysis1
Glycosylationi176N-linked (GlcNAc...)Sequence analysis1
Modified residuei386PhosphoserineBy similarity1
Modified residuei462PhosphothreonineBy similarity1
Modified residuei463PhosphothreonineBy similarity1
Modified residuei468PhosphothreonineBy similarity1
Modified residuei476PhosphotyrosineBy similarity1

Post-translational modificationi

Autophosphorylated.

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiC0LGF4.

Expressioni

Tissue specificityi

Expressed in the root meristem and elongation zone, and in hypocotyls of etiolated seedlings.1 Publication

Gene expression databases

ExpressionAtlasiC0LGF4. baseline and differential.
GenevisibleiC0LGF4. AT.

Interactioni

Subunit structurei

Interacts with the ACC synthases ACS5 and ACS9 but not ACS2, via the kinase domain.1 Publication

Protein-protein interaction databases

BioGridi25267. 35 interactors.
STRINGi3702.AT1G31420.1.

Structurei

3D structure databases

ProteinModelPortaliC0LGF4.
SMRiC0LGF4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati73 – 97LRR 1Add BLAST25
Repeati98 – 121LRR 2Add BLAST24
Repeati123 – 145LRR 3Add BLAST23
Repeati146 – 169LRR 4Add BLAST24
Repeati171 – 194LRR 5Add BLAST24
Domaini306 – 578Protein kinasePROSITE-ProRule annotationAdd BLAST273

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 5 LRR (leucine-rich) repeats.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Leucine-rich repeat, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIXE. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116554.
InParanoidiC0LGF4.
PhylomeDBiC0LGF4.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR031071. FEI1/2.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR27000:SF202. PTHR27000:SF202. 1 hit.
PfamiPF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced. According to EST sequences.
Isoform 1 (identifier: C0LGF4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMGICEMKSC CSWLLLISLL CSLSNESQAI SPDGEALLSF RNAVTRSDSF
60 70 80 90 100
IHQWRPEDPD PCNWNGVTCD AKTKRVITLN LTYHKIMGPL PPDIGKLDHL
110 120 130 140 150
RLLMLHNNAL YGAIPTALGN CTALEEIHLQ SNYFTGPIPA EMGDLPGLQK
160 170 180 190 200
LDMSSNTLSG PIPASLGQLK KLSNFNVSNN FLVGQIPSDG VLSGFSKNSF
210 220 230 240 250
IGNLNLCGKH VDVVCQDDSG NPSSHSQSGQ NQKKNSGKLL ISASATVGAL
260 270 280 290 300
LLVALMCFWG CFLYKKLGKV EIKSLAKDVG GGASIVMFHG DLPYSSKDII
310 320 330 340 350
KKLEMLNEEH IIGCGGFGTV YKLAMDDGKV FALKRILKLN EGFDRFFERE
360 370 380 390 400
LEILGSIKHR YLVNLRGYCN SPTSKLLLYD YLPGGSLDEA LHERGEQLDW
410 420 430 440 450
DSRVNIIIGA AKGLSYLHHD CSPRIIHRDI KSSNILLDGN LEARVSDFGL
460 470 480 490 500
AKLLEDEESH ITTIVAGTFG YLAPEYMQSG RATEKTDVYS FGVLVLEVLS
510 520 530 540 550
GKRPTDASFI EKGLNVVGWL KFLISEKRPR DIVDPNCEGM QMESLDALLS
560 570 580 590
IATQCVSPSP EERPTMHRVV QLLESEVMTP CPSEFYDSSS D
Length:591
Mass (Da):64,916
Last modified:May 5, 2009 - v1
Checksum:iEA57944B55157D91
GO

Sequence cautioni

The sequence AAG51266 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC027135 Genomic DNA. Translation: AAG51266.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE31354.1.
FJ708643 mRNA. Translation: ACN59239.1.
PIRiB86440.
RefSeqiNP_001185122.1. NM_001198193.1. [C0LGF4-1]
UniGeneiAt.27683.
At.67250.

Genome annotation databases

EnsemblPlantsiAT1G31420.2; AT1G31420.2; AT1G31420. [C0LGF4-1]
GeneIDi840032.
GrameneiAT1G31420.2; AT1G31420.2; AT1G31420.
KEGGiath:AT1G31420.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Web resourcesi

PlantP kinase Classification PPC

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC027135 Genomic DNA. Translation: AAG51266.1. Different initiation.
CP002684 Genomic DNA. Translation: AEE31354.1.
FJ708643 mRNA. Translation: ACN59239.1.
PIRiB86440.
RefSeqiNP_001185122.1. NM_001198193.1. [C0LGF4-1]
UniGeneiAt.27683.
At.67250.

3D structure databases

ProteinModelPortaliC0LGF4.
SMRiC0LGF4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi25267. 35 interactors.
STRINGi3702.AT1G31420.1.

Proteomic databases

PaxDbiC0LGF4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G31420.2; AT1G31420.2; AT1G31420. [C0LGF4-1]
GeneIDi840032.
GrameneiAT1G31420.2; AT1G31420.2; AT1G31420.
KEGGiath:AT1G31420.

Organism-specific databases

TAIRiAT1G31420.

Phylogenomic databases

eggNOGiENOG410IIXE. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000116554.
InParanoidiC0LGF4.
PhylomeDBiC0LGF4.

Miscellaneous databases

PROiC0LGF4.

Gene expression databases

ExpressionAtlasiC0LGF4. baseline and differential.
GenevisibleiC0LGF4. AT.

Family and domain databases

Gene3Di3.80.10.10. 1 hit.
InterProiIPR031071. FEI1/2.
IPR011009. Kinase-like_dom.
IPR032675. L_dom-like.
IPR013210. LRR_N_plant-typ.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR27000:SF202. PTHR27000:SF202. 1 hit.
PfamiPF08263. LRRNT_2. 1 hit.
PF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF52058. SSF52058. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFEI1_ARATH
AccessioniPrimary (citable) accession number: C0LGF4
Secondary accession number(s): Q9C867
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 3, 2009
Last sequence update: May 5, 2009
Last modified: November 2, 2016
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Miscellaneous

'Fei' means fat in Chinese.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.