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Protein

Tryptophan 2-C-methyltransferase

Gene

tsrM

Organism
Streptomyces laurentii
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthetic pathway of the antibiotic thiostrepton A. First, TsrM catalyzes the transfer of a methyl group from S-adenosyl methionine (SAM) to cobalamin, leading to the formation of methylcobalamin (CH3-cobalamin) and S-adenosyl-L-homocysteine (SAH). Then the methyl group is transferred to the C2 position of tryptophan (Trp) with the concerted action of the radical SAM [4Fe-4S] center, leading to the production of methyltryptophan.3 Publications

Miscellaneous

TsrM has a totally new mechanism among methyltransferases and radical SAM enzymes. TsrM is not able to use methylcobalamin directly but instead coordinates free cobalamin. SAM serves exclusively as a methyl donor and not as a radical source, and the methyl group of SAM is not directly transferred to tryptophan.1 Publication

Catalytic activityi

S-adenosyl-L-methionine + L-tryptophan = S-adenosyl-L-homocysteine + L-2-methyltryptophan.1 Publication

Cofactori

Protein has several cofactor binding sites:

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi253Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi257Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1
Metal bindingi260Iron-sulfur (4Fe-4S-S-AdoMet)1 Publication1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processAntibiotic biosynthesis
Ligand4Fe-4S, Cobalamin, Cobalt, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-19353.

Names & Taxonomyi

Protein namesi
Recommended name:
Tryptophan 2-C-methyltransferaseCurated (EC:2.1.1.1061 Publication)
Gene namesi
Name:tsrM1 Publication
Synonyms:trsT
OrganismiStreptomyces laurentii
Taxonomic identifieri39478 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaStreptomycetalesStreptomycetaceaeStreptomyces

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi253C → A: Loss of methyltransferase activity. 1 Publication1
Mutagenesisi257C → A: Loss of methyltransferase activity. 1 Publication1
Mutagenesisi260C → A: Loss of methyltransferase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004353081 – 599Tryptophan 2-C-methyltransferaseAdd BLAST599

Structurei

3D structure databases

ProteinModelPortaliC0JRZ9.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 149B12-bindingPROSITE-ProRule annotationAdd BLAST146

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
InterProiView protein in InterPro
IPR013785. Aldolase_TIM.
IPR030969. B12_rSAM_trp_MT.
IPR006158. Cobalamin-bd.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
PfamiView protein in Pfam
PF02310. B12-binding. 1 hit.
PF04055. Radical_SAM. 1 hit.
SFLDiSFLDF00322. tryptophan_2-C-methyltransfera. 1 hit.
SMARTiView protein in SMART
SM00729. Elp3. 1 hit.
TIGRFAMsiTIGR04428. B12_rSAM_trp_MT. 1 hit.
PROSITEiView protein in PROSITE
PS51332. B12_BINDING. 1 hit.

Sequencei

Sequence statusi: Complete.

C0JRZ9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLRKGTVALI NPNQIHPPIA PYALDVLTTA LEASGFEAHV LDLTFHLDDW
60 70 80 90 100
RQTLRDYFRA ERPLLVGVTC RNTDTVYALE QRPFVDGYKA VIDEVRRLTA
110 120 130 140 150
APVVAGGVGF STMPFALVDY FGIEYGVKGP GEKIICDLAR ALAEGRSADR
160 170 180 190 200
IHIPGLLVNR GPGNVTRVAP PALDPRAAPA PSSSPSPSPA PSSSSAPVPV
210 220 230 240 250
PLSFAAVGHH ESRAWQAETE LPYTRRSGEP YKVDNLRYYR EGGLGSILTK
260 270 280 290 300
NGCVYKCSFC VEPDAKGTQF ARRGITAVVD EMEALTAQGI HDLHTTDSEF
310 320 330 340 350
NLSIAHSKNL LREIVRRRDH DATSPLRDLR LWVYCQPSPF DEEFAELLAA
360 370 380 390 400
AGCAGVNIGA DHTRPEMLDG WKVTAKGTRY YDFADTERLV QLCHRNGMLT
410 420 430 440 450
MVEALFGMPG ETLETMRDCV DRMMELDATV TGFSLGLRLL PYMGLAKSLA
460 470 480 490 500
EQCDGVRTVR GLQSNNASGP IVLKQLHQCD GPIEYERQFM FDESGDFRLV
510 520 530 540 550
CYFSPDLPEA PGTADSPDGI WRASVDFLWD RIPKSEQYRV MLPTLSGSSE
560 570 580 590
NDNNYADNPF LTSLNRKGYT GAFWAHWRDR EAIMSGATLP LGELAEAVR
Length:599
Mass (Da):66,061
Last modified:May 5, 2009 - v1
Checksum:iA3829A2EEE2C851A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
FJ652572 Genomic DNA. Translation: ACN52303.1.
FJ436358 Genomic DNA. Translation: ACN80682.1.

Genome annotation databases

KEGGiag:ACN52303.

Similar proteinsi

Entry informationi

Entry nameiTSRM_STRLU
AccessioniPrimary (citable) accession number: C0JRZ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 20, 2016
Last sequence update: May 5, 2009
Last modified: April 12, 2017
This is version 32 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program