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Protein

Glucoamylase

Gene
N/A
Organism
Bacillus licheniformis
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Miscellaneous

On the 2D-gel the determined pI of this protein is: 5.0, its MW is: 185 kDa.1 Publication

Catalytic activityi

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose.1 Publication

Kineticsi

Because of the indeterminate molecular weight of starch, an approximate KM value of 0.2178 mg/ml was determined.1 Publication

      pH dependencei

      Optimum pH is 5.0.1 Publication

      Temperature dependencei

      Optimum temperature is 50 degrees Celsius.1 Publication

      GO - Molecular functioni

      GO - Biological processi

      Keywordsi

      Molecular functionGlycosidase, Hydrolase
      Biological processCarbohydrate metabolism, Polysaccharide degradation

      Names & Taxonomyi

      Protein namesi
      Recommended name:
      Glucoamylase1 Publication (EC:3.2.1.31 Publication)
      Alternative name(s):
      1,4-alpha-D-glucan glucohydrolaseBy similarity
      Glucan 1,4-alpha-glucosidaseBy similarity
      OrganismiBacillus licheniformis
      Taxonomic identifieri1402 [NCBI]
      Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus

      PTM / Processingi

      Molecule processing

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      ChainiPRO_00004241741 – ›10Glucoamylase›10

      Sequencei

      Sequence statusi: Fragment.

      Length:10
      Mass (Da):1,080
      Last modified:October 16, 2013 - v1
      Checksum:i18FABAA1B1A72334
      GO

      Experimental Info

      Feature keyPosition(s)DescriptionActionsGraphical viewLength
      Non-terminal residuei101 Publication1

      Entry informationi

      Entry nameiAMYG_BACLI
      AccessioniPrimary (citable) accession number: C0HJE2
      Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 16, 2013
      Last sequence update: October 16, 2013
      Last modified: October 1, 2014
      This is version 3 of the entry and version 1 of the sequence. See complete history.
      Entry statusiReviewed (UniProtKB/Swiss-Prot)
      Annotation programProkaryotic Protein Annotation Program

      Miscellaneousi

      Keywords - Technical termi

      Direct protein sequencing

      Documents

      1. Glycosyl hydrolases
        Classification of glycosyl hydrolase families and list of entries