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Protein

Laccase

Gene
N/A
Organism
Phellinus igniarius (Willow bracket) (Boletus igniarius)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products. Has highest activity for ABTS but can also act on guaiacol, catechol and hydroquinone with decreasing activity.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Kineticsi

  1. KM=1.31 mM for ABTS1 Publication

    pH dependencei

    Optimum pH is 2.4.1 Publication

    Temperature dependencei

    Optimum temperature is 20 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase1 Publication (EC:1.10.3.21 Publication)
    OrganismiPhellinus igniarius (Willow bracket) (Boletus igniarius)
    Taxonomic identifieri40472 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesHymenochaetalesHymenochaetaceaePhellinus

    Subcellular locationi

    • Secretedextracellular space 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›8›8LaccasePRO_0000422126

    Sequencei

    Sequence statusi: Fragment.

    Length:8
    Mass (Da):799
    Last modified:May 1, 2013 - v1
    Checksum:i87CB1AB2D768705D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei8 – 811 Publication

    Cross-referencesi

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    ProtoNetiSearch...

    Publicationsi

    1. "An extracellular laccase with antiproliferative activity from the medicinal fungi Phellinus igniarius."
      Sun J., Chen Q.J., Zhu J.M., Zhu X.M., Wang H.X., Zhang G.Q.
      Submitted (FEB-2013) to UniProtKB
      Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
      Strain: MW08011 Publication.
      Tissue: Mycelium1 Publication.

    Entry informationi

    Entry nameiLAC_PHEIG
    AccessioniPrimary (citable) accession number: C0HJB2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: May 1, 2013
    Last modified: January 7, 2015
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Inhibits cell proliferation of HepG2 hepatoma cell line with an IC50 of 2.6 µM and L1210 mouse leukemia cell line with an IC50=3.2 µM respectively.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    External Data

    Dasty 3

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.