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Protein

Laccase

Gene
N/A
Organism
Phellinus igniarius (Willow bracket) (Boletus igniarius)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products. Has highest activity for ABTS but can also act on guaiacol, catechol and hydroquinone with decreasing activity.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Kineticsi

  1. KM=1.31 mM for ABTS1 Publication

pH dependencei

Optimum pH is 2.4.1 Publication

Temperature dependencei

Optimum temperature is 20 degrees Celsius.1 Publication

GO - Molecular functioni

  1. hydroquinone:oxygen oxidoreductase activity Source: UniProtKB-EC

GO - Biological processi

  1. lignin catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Lignin degradation

Names & Taxonomyi

Protein namesi
Recommended name:
Laccase1 Publication (EC:1.10.3.21 Publication)
OrganismiPhellinus igniarius (Willow bracket) (Boletus igniarius)
Taxonomic identifieri40472 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesHymenochaetalesHymenochaetaceaePhellinus

Subcellular locationi

  1. Secretedextracellular space 1 Publication

GO - Cellular componenti

  1. extracellular space Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – ›8›8LaccasePRO_0000422126

Sequencei

Sequence statusi: Fragment.

Length:8
Mass (Da):799
Last modified:May 1, 2013 - v1
Checksum:i87CB1AB2D768705D
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei8 – 811 Publication

Cross-referencesi

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Publicationsi

  1. "An extracellular laccase with antiproliferative activity from the medicinal fungi Phellinus igniarius."
    Sun J., Chen Q.J., Zhu J.M., Zhu X.M., Wang H.X., Zhang G.Q.
    Submitted (FEB-2013) to UniProtKB
    Cited for: PROTEIN SEQUENCE, FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION.
    Strain: MW08011 Publication.
    Tissue: Mycelium1 Publication.

Entry informationi

Entry nameiLAC_PHEIG
AccessioniPrimary (citable) accession number: C0HJB2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 1, 2013
Last sequence update: May 1, 2013
Last modified: January 7, 2015
This is version 6 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Inhibits cell proliferation of HepG2 hepatoma cell line with an IC50 of 2.6 µM and L1210 mouse leukemia cell line with an IC50=3.2 µM respectively.1 Publication

Keywords - Technical termi

Direct protein sequencing

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.