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Protein

Laccase

Gene
N/A
Organism
Phellinus igniarius (Willow bracket) (Boletus igniarius)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Lignin degradation and detoxification of lignin-derived products. Has highest activity for ABTS but can also act on guaiacol, catechol and hydroquinone with decreasing activity.1 Publication

Catalytic activityi

4 benzenediol + O2 = 4 benzosemiquinone + 2 H2O.1 Publication

Kineticsi

  1. KM=1.31 mM for ABTS1 Publication

    pH dependencei

    Optimum pH is 2.4.1 Publication

    Temperature dependencei

    Optimum temperature is 20 degrees Celsius.1 Publication

    GO - Molecular functioni

    GO - Biological processi

    Complete GO annotation...

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Lignin degradation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Laccase1 Publication (EC:1.10.3.21 Publication)
    OrganismiPhellinus igniarius (Willow bracket) (Boletus igniarius)
    Taxonomic identifieri40472 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaBasidiomycotaAgaricomycotinaAgaricomycetesHymenochaetalesHymenochaetaceaePhellinus

    Subcellular locationi

    • Secretedextracellular space 1 Publication

    GO - Cellular componenti

    Complete GO annotation...

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – ›8›8LaccasePRO_0000422126

    Sequencei

    Sequence statusi: Fragment.

    Length:8
    Mass (Da):799
    Last modified:May 1, 2013 - v1
    Checksum:i87CB1AB2D768705D
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei8 – 811 Publication

    Cross-referencesi

    3D structure databases

    ModBaseiSearch...
    MobiDBiSearch...

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Family and domain databases

    ProtoNetiSearch...

    Entry informationi

    Entry nameiLAC_PHEIG
    AccessioniPrimary (citable) accession number: C0HJB2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 1, 2013
    Last sequence update: May 1, 2013
    Last modified: January 7, 2015
    This is version 6 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Inhibits cell proliferation of HepG2 hepatoma cell line with an IC50 of 2.6 µM and L1210 mouse leukemia cell line with an IC50=3.2 µM respectively.1 Publication

    Keywords - Technical termi

    Direct protein sequencing

    Similar proteinsi

    Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
    100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
    90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
    50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.