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Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

usp12

Organism
Salmo salar (Atlantic salmon)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei48NucleophilePROSITE-ProRule annotation1
Metal bindingi188ZincBy similarity1
Metal bindingi191ZincBy similarity1
Metal bindingi235ZincBy similarity1
Metal bindingi238ZincBy similarity1
Active sitei319Proton acceptorPROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Protein family/group databases

MEROPSiC19.062.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12
Gene namesi
Name:usp12
OrganismiSalmo salar (Atlantic salmon)
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo
Proteomesi
  • UP000087266 Componenti: Genome assembly

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003789921 – 372Ubiquitin carboxyl-terminal hydrolase 12Add BLAST372

Interactioni

Subunit structurei

Interacts with WDR48.By similarity

Structurei

3D structure databases

SMRiC0HB46.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 371USPAdd BLAST333

Sequence similaritiesi

Phylogenomic databases

KOiK11842.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.

Sequencei

Sequence statusi: Complete.

C0HB46-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEILMTVSKF ASFCTMGANA SALEKEIGSE QFPVNEHYFG LVNFGNTCYC
60 70 80 90 100
NSVLQALYFC RPFREKILAY RSQPRRKENL LTCLADLFHS IANQKRKVGV
110 120 130 140 150
IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADLL QEERKQDKTN
160 170 180 190 200
GRLANGSLDS QNHNSNAPPP STWVHEIFQG TLTNETRCLT CETISSKDED
210 220 230 240 250
FLDLSVDVEQ NTSITHCLRG FSNTETLCSE YKYYCEECRS KQEAHKRMRV
260 270 280 290 300
KKLPMILALH LKRFKYMEQL QRYTKLSYRV VFPLELRLFN TSGDATNPER
310 320 330 340 350
LYDLVAVVVH CGSGPNRGHY IAIVKSHDFW LLFDDDIVEK IDAQAIEEFY
360 370
GLTSEISKNS ESGYILFYQS RD
Length:372
Mass (Da):43,117
Last modified:May 5, 2009 - v1
Checksum:i0C3294DF7CEC4C18
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti163H → N in ACI33173 (PubMed:20433749).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT044911 mRNA. Translation: ACI33173.1.
BT059552 mRNA. Translation: ACN11265.1.
RefSeqiNP_001133405.1. NM_001139933.1.
XP_014014921.1. XM_014159446.1.
XP_014036980.1. XM_014181505.1.
UniGeneiSsa.17383.
Ssa.7010.

Genome annotation databases

GeneIDi100194904.
106590448.
KEGGisasa:100194904.
sasa:106590448.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT044911 mRNA. Translation: ACI33173.1.
BT059552 mRNA. Translation: ACN11265.1.
RefSeqiNP_001133405.1. NM_001139933.1.
XP_014014921.1. XM_014159446.1.
XP_014036980.1. XM_014181505.1.
UniGeneiSsa.17383.
Ssa.7010.

3D structure databases

SMRiC0HB46.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100194904.
106590448.
KEGGisasa:100194904.
sasa:106590448.

Organism-specific databases

CTDi219333.

Phylogenomic databases

KOiK11842.

Family and domain databases

InterProiView protein in InterPro
IPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
PfamiView protein in Pfam
PF00443. UCH. 1 hit.
PROSITEiView protein in PROSITE
PS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiUBP12_SALSA
AccessioniPrimary (citable) accession number: C0HB46
Secondary accession number(s): B5X1J2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 5, 2009
Last modified: June 7, 2017
This is version 28 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.