C0HB46 (UBP12_SALSA) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12
EC=3.4.19.12

Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12

Gene names

Name:

usp12

Organism

Salmo salar (Atlantic salmon)

Taxonomic identifier

8030 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Elopocephala › Clupeocephala › Euteleostei › Protacanthopterygii › Salmoniformes › Salmonoidei › Salmonidae › Salmoninae › Salmo

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity By similarity.
Catalytic activity	Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
Sequence similarities	Belongs to the peptidase C19 family. USP12/USP46 subfamily.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Molecular function	Hydrolase Protease Thiol protease
Gene Ontology (GO)
Biological_process	protein deubiquitination Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Molecular_function	cysteine-type endopeptidase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin thiolesterase activity Inferred from sequence or structural similarity. Source: UniProtKB ubiquitin-specific protease activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Ubiquitin carboxyl-terminal hydrolase 12		PRO_0000378992
Sites
	Active site	48	1	Nucleophile By similarity
	Active site	319	1	Proton acceptor By similarity
Experimental info
	Sequence conflict	163	1	H → N in ACI33173. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	C0HB46 [UniParc]. Last modified May 5, 2009. Version 1. Checksum: 0C3294DF7CEC4C18	FASTA	372	43,117
	10 20 30 40 50 60 MEILMTVSKF ASFCTMGANA SALEKEIGSE QFPVNEHYFG LVNFGNTCYC NSVLQALYFC 70 80 90 100 110 120 RPFREKILAY RSQPRRKENL LTCLADLFHS IANQKRKVGV IPPKKFITRL RKENELFDNY 130 140 150 160 170 180 MQQDAHEFLN YLLNTIADLL QEERKQDKTN GRLANGSLDS QNHNSNAPPP STWVHEIFQG 190 200 210 220 230 240 TLTNETRCLT CETISSKDED FLDLSVDVEQ NTSITHCLRG FSNTETLCSE YKYYCEECRS 250 260 270 280 290 300 KQEAHKRMRV KKLPMILALH LKRFKYMEQL QRYTKLSYRV VFPLELRLFN TSGDATNPER 310 320 330 340 350 360 LYDLVAVVVH CGSGPNRGHY IAIVKSHDFW LLFDDDIVEK IDAQAIEEFY GLTSEISKNS 370 ESGYILFYQS RD

References

[1]

"Salmo salar and Esox lucius full-length cDNA sequences reveal changes in evolutionary pressures on a post-tetraploidization genome." Leong J.S., Jantzen S.G., von Schalburg K.R., Cooper G.A., Messmer A.M., Liao N.Y., Munro S., Moore R., Holt R.A., Jones S.J., Davidson W.S., Koop B.F. BMC Genomics 11:279-279(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BT044911 mRNA. Translation: ACI33173.1. BT059552 mRNA. Translation: ACN11265.1.
RefSeq	NP_001133405.1. NM_001139933.1.
UniGene	Ssa.17383.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100194904.
Organism-specific databases
CTD	100194904.
Family and domain databases
InterPro	IPR018200. Pept_C19ubi-hydrolase_C_CS. IPR001394. Peptidase_C19. [Graphical view]
Pfam	PF00443. UCH. 1 hit. [Graphical view]
PROSITE	PS00972. UCH_2_1. 1 hit. PS00973. UCH_2_2. 1 hit. PS50235. UCH_2_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UBP12_SALSA

Accession

Primary (citable) accession number: C0HB46
Secondary accession number(s): B5X1J2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 7, 2009
Last sequence update:	May 5, 2009
Last modified:	February 22, 2012
This is version 11 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program