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Protein

Ubiquitin carboxyl-terminal hydrolase 12

Gene

usp12

Organism
Salmo salar (Atlantic salmon)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Deubiquitinating enzyme. Has almost no deubiquitinating activity by itself and requires the interaction with wdr48 to have a high activity (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei48 – 481NucleophilePROSITE-ProRule annotation
Active sitei319 – 3191Proton acceptorPROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type endopeptidase activity Source: UniProtKB
  2. ubiquitin-specific protease activity Source: UniProtKB
  3. ubiquitin thiolesterase activity Source: UniProtKB

GO - Biological processi

  1. protein deubiquitination Source: UniProtKB
  2. ubiquitin-dependent protein catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Ubl conjugation pathway

Protein family/group databases

MEROPSiC19.062.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin carboxyl-terminal hydrolase 12 (EC:3.4.19.12)
Alternative name(s):
Deubiquitinating enzyme 12
Ubiquitin thioesterase 12
Ubiquitin-specific-processing protease 12
Gene namesi
Name:usp12
OrganismiSalmo salar (Atlantic salmon)
Taxonomic identifieri8030 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiProtacanthopterygiiSalmoniformesSalmonidaeSalmoninaeSalmo

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 372372Ubiquitin carboxyl-terminal hydrolase 12PRO_0000378992Add
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini39 – 371333USPAdd
BLAST

Sequence similaritiesi

Contains 1 USP domain.Curated

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0HB46-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEILMTVSKF ASFCTMGANA SALEKEIGSE QFPVNEHYFG LVNFGNTCYC
60 70 80 90 100
NSVLQALYFC RPFREKILAY RSQPRRKENL LTCLADLFHS IANQKRKVGV
110 120 130 140 150
IPPKKFITRL RKENELFDNY MQQDAHEFLN YLLNTIADLL QEERKQDKTN
160 170 180 190 200
GRLANGSLDS QNHNSNAPPP STWVHEIFQG TLTNETRCLT CETISSKDED
210 220 230 240 250
FLDLSVDVEQ NTSITHCLRG FSNTETLCSE YKYYCEECRS KQEAHKRMRV
260 270 280 290 300
KKLPMILALH LKRFKYMEQL QRYTKLSYRV VFPLELRLFN TSGDATNPER
310 320 330 340 350
LYDLVAVVVH CGSGPNRGHY IAIVKSHDFW LLFDDDIVEK IDAQAIEEFY
360 370
GLTSEISKNS ESGYILFYQS RD
Length:372
Mass (Da):43,117
Last modified:May 5, 2009 - v1
Checksum:i0C3294DF7CEC4C18
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti163 – 1631H → N in ACI33173. (PubMed:20433749)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT044911 mRNA. Translation: ACI33173.1.
BT059552 mRNA. Translation: ACN11265.1.
RefSeqiNP_001133405.1. NM_001139933.1.
UniGeneiSsa.17383.

Genome annotation databases

GeneIDi100194904.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BT044911 mRNA. Translation: ACI33173.1.
BT059552 mRNA. Translation: ACN11265.1.
RefSeqiNP_001133405.1. NM_001139933.1.
UniGeneiSsa.17383.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

MEROPSiC19.062.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100194904.

Organism-specific databases

CTDi100194904.

Family and domain databases

InterProiIPR001394. Peptidase_C19_UCH.
IPR018200. USP_CS.
IPR028889. USP_dom.
[Graphical view]
PfamiPF00443. UCH. 1 hit.
[Graphical view]
PROSITEiPS00972. USP_1. 1 hit.
PS00973. USP_2. 1 hit.
PS50235. USP_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Salmo salar and Esox lucius full-length cDNA sequences reveal changes in evolutionary pressures on a post-tetraploidization genome."
    Leong J.S., Jantzen S.G., von Schalburg K.R., Cooper G.A., Messmer A.M., Liao N.Y., Munro S., Moore R., Holt R.A., Jones S.J., Davidson W.S., Koop B.F.
    BMC Genomics 11:279-279(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.

Entry informationi

Entry nameiUBP12_SALSA
AccessioniPrimary (citable) accession number: C0HB46
Secondary accession number(s): B5X1J2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 7, 2009
Last sequence update: May 5, 2009
Last modified: January 7, 2015
This is version 18 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.