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Protein

Mannitol-specific phosphotransferase enzyme IIA component

Gene

mtlF

Organism
Bacillus subtilis (strain 168)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in mannitol transport (By similarity).By similarity

Catalytic activityi

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei62 – 621Tele-phosphohistidine intermediatePROSITE-ProRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Phosphotransferase system, Sugar transport, Transport

Enzyme and pathway databases

BioCyciBSUB:BSU03982-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mannitol-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
Alternative name(s):
EIIA-Mtl
PTS system mannitol-specific EIIA component
Gene namesi
Name:mtlF
Ordered Locus Names:BSU03982
ORF Names:BSU03980
OrganismiBacillus subtilis (strain 168)
Taxonomic identifieri224308 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillus
Proteomesi
  • UP000001570 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 143143Mannitol-specific phosphotransferase enzyme IIA componentPRO_0000376996Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei74 – 741Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiC0H3V2.

PTM databases

iPTMnetiC0H3V2.

Interactioni

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100002248.

Structurei

3D structure databases

ProteinModelPortaliC0H3V2.
SMRiC0H3V2. Positions 4-141.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini2 – 142141PTS EIIA type-2PROSITE-ProRule annotationAdd
BLAST

Domaini

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.

Sequence similaritiesi

Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG2213. LUCA.
COG4668. LUCA.
HOGENOMiHOG000227563.
KOiK02798.
OMAiISIVQVP.
PhylomeDBiC0H3V2.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

C0H3V2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQVLAKENIK LNQTVSSKEE AIKLAGQTLI DNGYVTEDYI SKMFEREETS
60 70 80 90 100
STFMGNFIAI PHGTEEAKSE VLHSGISIIQ IPEGVEYGEG NTAKVVFGIA
110 120 130 140
GKNNEHLDIL SNIAIICSEE ENIERLISAK SEEDLIAIFN EVN
Length:143
Mass (Da):15,747
Last modified:May 5, 2009 - v1
Checksum:iA107403396C2548C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAX52549.1.
RefSeqiWP_003246714.1. NZ_JNCM01000031.1.
YP_003097678.1. NC_000964.3.

Genome annotation databases

EnsemblBacteriaiCAX52549; CAX52549; BSU03982.
GeneIDi8303136.
KEGGibsu:BSU03982.
PATRICi18972372. VBIBacSub10457_0414.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL009126 Genomic DNA. Translation: CAX52549.1.
RefSeqiWP_003246714.1. NZ_JNCM01000031.1.
YP_003097678.1. NC_000964.3.

3D structure databases

ProteinModelPortaliC0H3V2.
SMRiC0H3V2. Positions 4-141.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi224308.Bsubs1_010100002248.

PTM databases

iPTMnetiC0H3V2.

Proteomic databases

PaxDbiC0H3V2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAX52549; CAX52549; BSU03982.
GeneIDi8303136.
KEGGibsu:BSU03982.
PATRICi18972372. VBIBacSub10457_0414.

Phylogenomic databases

eggNOGiCOG2213. LUCA.
COG4668. LUCA.
HOGENOMiHOG000227563.
KOiK02798.
OMAiISIVQVP.
PhylomeDBiC0H3V2.

Enzyme and pathway databases

BioCyciBSUB:BSU03982-MONOMER.

Family and domain databases

Gene3Di3.40.930.10. 1 hit.
InterProiIPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
[Graphical view]
PfamiPF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
SUPFAMiSSF55804. SSF55804. 1 hit.
PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPTMA_BACSU
AccessioniPrimary (citable) accession number: C0H3V2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2009
Last sequence update: May 5, 2009
Last modified: September 7, 2016
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Bacillus subtilis
    Bacillus subtilis (strain 168): entries, gene names and cross-references to SubtiList
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.