ID   STBOS_STERE             Reviewed;         761 AA.
AC   B9X0J1;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   14-APR-2009, sequence version 1.
DT   13-SEP-2023, entry version 39.
DE   RecName: Full=Baccharis oxide synthase;
DE            Short=StrBOS;
DE            EC=5.4.99.51;
OS   Stevia rebaudiana (Stevia) (Eupatorium rebaudianum).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Eupatorieae; Stevia.
OX   NCBI_TaxID=55670;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RC   TISSUE=Root;
RX   PubMed=18850716; DOI=10.1021/ol802072y;
RA   Shibuya M., Sagara A., Saitoh A., Kushiro T., Ebizuka Y.;
RT   "Biosynthesis of baccharis oxide, a triterpene with a 3,10-oxide bridge in
RT   the A-ring.";
RL   Org. Lett. 10:5071-5074(2008).
CC   -!- FUNCTION: Oxidosqualene cyclase converting (3S)-2,3-epoxy-2,3-
CC       dihydrosqualene to baccharis oxide, a triterpene with a 3,10-oxide
CC       bridge in the A-ring. Also mediates conversion of other triterpenoids
CC       at a much lower level. {ECO:0000269|PubMed:18850716}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(S)-2,3-epoxysqualene = baccharis oxide; Xref=Rhea:RHEA:31867,
CC         ChEBI:CHEBI:15441, ChEBI:CHEBI:63463; EC=5.4.99.51;
CC         Evidence={ECO:0000269|PubMed:18850716};
CC   -!- SIMILARITY: Belongs to the terpene cyclase/mutase family.
CC       {ECO:0000305}.
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DR   EMBL; AB455264; BAH23676.1; -; mRNA.
DR   AlphaFoldDB; B9X0J1; -.
DR   SMR; B9X0J1; -.
DR   KEGG; ag:BAH23676; -.
DR   GO; GO:0005811; C:lipid droplet; IEA:InterPro.
DR   GO; GO:0031559; F:oxidosqualene cyclase activity; IDA:UniProtKB.
DR   GO; GO:0016104; P:triterpenoid biosynthetic process; IEA:InterPro.
DR   CDD; cd02892; SQCY_1; 1.
DR   Gene3D; 1.50.10.20; -; 2.
DR   InterPro; IPR032696; SQ_cyclase_C.
DR   InterPro; IPR032697; SQ_cyclase_N.
DR   InterPro; IPR018333; Squalene_cyclase.
DR   InterPro; IPR002365; Terpene_synthase_CS.
DR   InterPro; IPR008930; Terpenoid_cyclase/PrenylTrfase.
DR   NCBIfam; TIGR01787; squalene_cyclas; 1.
DR   PANTHER; PTHR11764:SF58; BETA-AMYRIN SYNTHASE-RELATED; 1.
DR   PANTHER; PTHR11764; TERPENE CYCLASE/MUTASE FAMILY MEMBER; 1.
DR   Pfam; PF13243; SQHop_cyclase_C; 1.
DR   Pfam; PF13249; SQHop_cyclase_N; 1.
DR   SFLD; SFLDG01016; Prenyltransferase_Like_2; 1.
DR   SUPFAM; SSF48239; Terpenoid cyclases/Protein prenyltransferases; 2.
DR   PROSITE; PS01074; TERPENE_SYNTHASES; 1.
PE   1: Evidence at protein level;
KW   Isomerase; Repeat.
FT   CHAIN           1..761
FT                   /note="Baccharis oxide synthase"
FT                   /id="PRO_0000418758"
FT   REPEAT          148..189
FT                   /note="PFTB 1"
FT   REPEAT          639..680
FT                   /note="PFTB 2"
FT   ACT_SITE        484
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:P48449"
SQ   SEQUENCE   761 AA;  88050 MW;  79354A8BA097B2C2 CRC64;
     MWRLKIADGN NNPYLYSTNN FVGRQTWEFD PNYGTQEERD EVEQARQHFW NNRHQFKATG
     DVLWRMQFIR EKRFKQTIPQ VKIEDGEEIS YDKVTATLRR SVHLLAALQA DDGHWPAENT
     GPMFFIQPLV ICLYITGHLD RVFPKEHKKE ILRYLYTQQN EDGGWGLHIE GQSIMFGTIM
     SYVCMRLLGE GPDGGLNGAC TKARKWILDH GSVLASPSWG KIYLTILGVH EWEGCNPLPP
     EFWILPSIFP MHPAKMWCYC RLIYMPMSYL YGRRFVGPIT PLVLQLREEL YSQSYNDIKW
     KSTRHLVVKE DLHYPHPWLQ DLMWDGLYIF TEPLLTRWPF SKLREKALKT TINHIHYEDE
     NSRYITIGAV EKSLCMLACW DEDPDGVCFK KHLARIPDYI WVSEDGLKMQ SFGSQLWDAS
     LAIQALLATD LNHDIEPILR KGHDFIKASQ VKDNPSGDFK SMYRHITKGS WTFSDQDHGW
     QTSDCTTEGL KCCLLLSKMS AEIVGEKMQP EQFYDAVNLI LSLQCKNGGE AGWEPAGESN
     WLEFLNPSEL FEDIVLEHDS VECTATGMQA LVIFKKLYPK HRREEIEKFL KDACGYLEKV
     QMQDGSWYGE WGICFTYGTC FALGGMEAIG KTYENCEAIR RAVNFLLTTQ RNDGGWGESY
     RSSPKKKYVP LEGNRSNLVQ TACALMGLIR SKQEERDPTP LHRAAKLLIN SQMENGDFPQ
     EETGGVFKKN CLLHYPMYRN IYTLWALGEY RKKVLPQPTK V
//