B9X0J1 (STBOS_STERE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Baccharis oxide synthase Short name=StrBOS EC=5.4.99.51
Organism	Stevia rebaudiana (Stevia)
Taxonomic identifier	55670 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › campanulids › Asterales › Asteraceae › Asteroideae › Heliantheae alliance › Eupatorieae › Stevia

Protein attributes

Sequence length	761 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Oxidosqualene cyclase converting (3S)-2,3-epoxy-2,3-dihydrosqualene to baccharis oxide, a triterpene with a 3,10-oxide bridge in the A-ring. Also mediates conversion of other triterpenoids at a much lower level. Ref.1
Catalytic activity	(3S)-2,3-epoxy-2,3-dihydrosqualene = baccharis oxide. Ref.1
Sequence similarities	Belongs to the terpene cyclase/mutase family. Contains 2 PFTB repeats.

Ontologies

Keywords
Domain	Repeat
Molecular function	Isomerase
Gene Ontology (GO)
Molecular_function	oxidosqualene cyclase activity Inferred from direct assay Ref.1. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 761	761	Baccharis oxide synthase		PRO_0000418758
Regions
	Repeat	148 – 189	42	PFTB 1
	Repeat	639 – 680	42	PFTB 2

Sequences

	Sequence		Length	Mass (Da)
	B9X0J1 [UniParc]. Last modified April 14, 2009. Version 1. Checksum: 79354A8BA097B2C2	FASTA	761	88,050
	10 20 30 40 50 60 MWRLKIADGN NNPYLYSTNN FVGRQTWEFD PNYGTQEERD EVEQARQHFW NNRHQFKATG 70 80 90 100 110 120 DVLWRMQFIR EKRFKQTIPQ VKIEDGEEIS YDKVTATLRR SVHLLAALQA DDGHWPAENT 130 140 150 160 170 180 GPMFFIQPLV ICLYITGHLD RVFPKEHKKE ILRYLYTQQN EDGGWGLHIE GQSIMFGTIM 190 200 210 220 230 240 SYVCMRLLGE GPDGGLNGAC TKARKWILDH GSVLASPSWG KIYLTILGVH EWEGCNPLPP 250 260 270 280 290 300 EFWILPSIFP MHPAKMWCYC RLIYMPMSYL YGRRFVGPIT PLVLQLREEL YSQSYNDIKW 310 320 330 340 350 360 KSTRHLVVKE DLHYPHPWLQ DLMWDGLYIF TEPLLTRWPF SKLREKALKT TINHIHYEDE 370 380 390 400 410 420 NSRYITIGAV EKSLCMLACW DEDPDGVCFK KHLARIPDYI WVSEDGLKMQ SFGSQLWDAS 430 440 450 460 470 480 LAIQALLATD LNHDIEPILR KGHDFIKASQ VKDNPSGDFK SMYRHITKGS WTFSDQDHGW 490 500 510 520 530 540 QTSDCTTEGL KCCLLLSKMS AEIVGEKMQP EQFYDAVNLI LSLQCKNGGE AGWEPAGESN 550 560 570 580 590 600 WLEFLNPSEL FEDIVLEHDS VECTATGMQA LVIFKKLYPK HRREEIEKFL KDACGYLEKV 610 620 630 640 650 660 QMQDGSWYGE WGICFTYGTC FALGGMEAIG KTYENCEAIR RAVNFLLTTQ RNDGGWGESY 670 680 690 700 710 720 RSSPKKKYVP LEGNRSNLVQ TACALMGLIR SKQEERDPTP LHRAAKLLIN SQMENGDFPQ 730 740 750 760 EETGGVFKKN CLLHYPMYRN IYTLWALGEY RKKVLPQPTK V

References

[1]	"Biosynthesis of baccharis oxide, a triterpene with a 3,10-oxide bridge in the A-ring." Shibuya M., Sagara A., Saitoh A., Kushiro T., Ebizuka Y. Org. Lett. 10:5071-5074(2008) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, CATALYTIC ACTIVITY. Tissue: Root.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB455264 mRNA. Translation: BAH23676.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR001330. Prenyltrans. IPR018333. Squalene_cyclase. IPR002365. Terpene_synthase_CS. IPR008930. Terpenoid_cyclase/PrenylTrfase. [Graphical view]
Pfam	PF00432. Prenyltrans. 1 hit. [Graphical view]
SUPFAM	SSF48239. Terp_cyc_toroid. 2 hits.
TIGRFAMs	TIGR01787. squalene_cyclas. 1 hit.
PROSITE	PS01074. TERPENE_SYNTHASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

STBOS_STERE

Accession

Primary (citable) accession number: B9X0J1

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 5, 2012
Last sequence update:	April 14, 2009
Last modified:	October 3, 2012
This is version 12 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program