B9WJJ7 (KEX1_CANDC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Pheromone-processing carboxypeptidase KEX1
EC=3.4.16.6

Alternative name(s):
Carboxypeptidase D

Gene names

Name:	KEX1
ORF Names:	CD36_70870

Organism

Candida dubliniensis (strain CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841) (Yeast) [Complete proteome]

Taxonomic identifier

573826 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › mitosporic Saccharomycetales › Candida › Candida dubliniensis

Protein attributes

Sequence length	686 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from protein precursors. Promotes cell fusion and is involved in the programmed cell death By similarity.
Catalytic activity	Preferential release of a C-terminal arginine or lysine residue.
Subcellular location	Golgi apparatus › trans-Golgi network membrane; Single-pass type I membrane protein By similarity.
Sequence similarities	Belongs to the peptidase S10 family.

Ontologies

Keywords
Biological process	Apoptosis
Cellular component	Golgi apparatus Membrane
Domain	Signal Transmembrane Transmembrane helix
Molecular function	Carboxypeptidase Hydrolase Protease
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	apoptotic process Inferred from electronic annotation. Source: UniProtKB-KW proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	serine-type carboxypeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	Potential
	Chain	20 – 686	667	Pheromone-processing carboxypeptidase KEX1		PRO_0000411909
Regions
	Topological domain	20 – 543	524	Lumenal Potential
	Transmembrane	544 – 564	21	Helical; Potential
	Topological domain	565 – 686	122	Cytoplasmic Potential
	Compositional bias	522 – 530	9	Poly-Ser
Sites
	Active site	184	1	By similarity
	Active site	394	1	By similarity
	Active site	452	1	By similarity
Amino acid modifications
	Glycosylation	85	1	N-linked (GlcNAc...) Potential
	Glycosylation	122	1	N-linked (GlcNAc...) Potential
	Glycosylation	441	1	N-linked (GlcNAc...) Potential
	Glycosylation	449	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	B9WJJ7 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: D6525FC9603CA262	FASTA	686	77,574
	10 20 30 40 50 60 MKFLLPTFII FIYTLLVSAL PTKEGSDPKA AKKYLVSDLP GLHDNITPDD SIPLMFAGQL 70 80 90 100 110 120 EIYPESNTHY FFWKFSDSNQ ETITNRTIFW LNGGPGCSSM DGALLETGPF RINSQQQVIS 130 140 150 160 170 180 NNGSWHKSGD IIYVDQPAGT GFSYSDTYIT DLDQVANYFL KFMEAYYELF PQEINNEIYF 190 200 210 220 230 240 AGESYAGQYI PYIANAILQR NKKLHEGEQK YDLRGVLIGN GWVSPNEQSL SYLPFFKDHG 250 260 270 280 290 300 LIDIHHPKWA TLLAKHEQCQ KIVNKIDSTF DDGTVHYYEV SSSTCEAILT DLLEYTQDTA 310 320 330 340 350 360 NDKNQQCINM YDYTLRDSYP SCGMNWPNEL VNVGPFLRQE KVMHQLNLIN LKKWNECNGK 370 380 390 400 410 420 VGRTFQARHS IPSVHLLPEL AKEIPVMLFN GANDIICNSQ GVLSYLQKLQ WNGETGFINK 430 440 450 460 470 480 DNQISWVYDN KEVGYMLWER NISFINIYNS SHMVPYDLPD VSRALIDLIT GEYDEKDVDG 490 500 510 520 530 540 KKSFVTYPLG SRKENDESKN PVESPSQTID PIISSSSSSV ESSLSSSSAS ATDSDSTSSK 550 560 570 580 590 600 FTRLIQLAVI LVIFWGVYVL YASYKSRPSS IIKKPTNNTS NITRSSTGKK KNVQWADQLN 610 620 630 640 650 660 QFEDDERNQE SNQGIIAKAI GKITGSKDTR GRYAPVHREN DNEYIDDIEL GEGISDPNVD 670 680 EFIIGSDDDD EEEQPRSDPA THKSVS

References

[1]

"Comparative genomics of the fungal pathogens Candida dubliniensis and Candida albicans." Jackson A.P., Gamble J.A., Yeomans T., Moran G.P., Saunders D., Harris D., Aslett M., Barrell J.F., Butler G., Citiulo F., Coleman D.C., de Groot P.W.J., Goodwin T.J., Quail M.A., McQuillan J., Munro C.A., Pain A., Poulter R.T. , Rajandream M.A., Renauld H., Spiering M.J., Tivey A., Gow N.A.R., Barrell B., Sullivan D.J., Berriman M. Genome Res. 19:2231-2244(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CD36 / ATCC MYA-646 / CBS 7987 / NCPF 3949 / NRRL Y-17841.

Cross-references

Sequence databases
EMBL GenBank DDBJ	FM992694 Genomic DNA. Translation: CAX40641.1.
RefSeq	XP_002421307.1. XM_002421262.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	573826.CD36_70870.
Protein family/group databases
MEROPS	S10.007.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8049274.
KEGG	cdu:CD36_70870.
Phylogenomic databases
eggNOG	COG2939.
KO	K01288.
OrthoDB	EOG44QX8Z.
Family and domain databases
InterPro	IPR001563. Peptidase_S10. IPR018202. Peptidase_S10_AS. [Graphical view]
PANTHER	PTHR11802. PTHR11802. 1 hit.
Pfam	PF00450. Peptidase_S10. 1 hit. [Graphical view]
PRINTS	PR00724. CRBOXYPTASEC.
PROSITE	PS00560. CARBOXYPEPT_SER_HIS. 1 hit. PS00131. CARBOXYPEPT_SER_SER. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KEX1_CANDC

Accession

Primary (citable) accession number: B9WJJ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 27, 2011
Last sequence update:	March 24, 2009
Last modified:	April 3, 2013
This is version 21 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program