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B9RX57

- LISC_RICCO

UniProt

B9RX57 - LISC_RICCO

Protein

Lipoyl synthase, chloroplastic

Gene

LIP1P

Organism
Ricinus communis (Castor bean)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 33 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.UniRule annotation

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi95 – 951Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi100 – 1001Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi106 – 1061Iron-sulfur 1 (4Fe-4S)UniRule annotation
    Metal bindingi126 – 1261Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi130 – 1301Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation
    Metal bindingi133 – 1331Iron-sulfur 2 (4Fe-4S-S-AdoMet)UniRule annotation

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-HAMAP
    2. lipoate synthase activity Source: UniProtKB-HAMAP
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. protein lipoylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, chloroplasticUniRule annotation (EC:2.8.1.8UniRule annotation)
    Alternative name(s):
    Lipoate synthaseUniRule annotation
    Short name:
    LSUniRule annotation
    Short name:
    Lip-synUniRule annotation
    Lipoate synthase, plastidialUniRule annotation
    Short name:
    LIP1pUniRule annotation
    Lipoic acid synthaseUniRule annotation
    Gene namesi
    Name:LIP1PUniRule annotation
    ORF Names:RCOM_0817880
    OrganismiRicinus communis (Castor bean)
    Taxonomic identifieri3988 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus
    ProteomesiUP000008311: Unassembled WGS sequence

    Subcellular locationi

    Plastidchloroplast UniRule annotation

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 7070ChloroplastUniRule annotationAdd
    BLAST
    Chaini71 – 364294Lipoyl synthase, chloroplasticPRO_0000398869Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliB9RX57.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the radical SAM superfamily. Lipoyl synthase family.UniRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B9RX57-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQTLFNPSI SMPKSFYHKH ITISSRIQCQ LSTNSPSSNT KTTTVTVPSK    50
    KTMGPYTGRD PNVKKPEWLR QRAPQGERFQ EVKHSLSSLK LNTVCEEAQC 100
    PNIGECWNGG GDGIATATIM LLGDTCTRGC RFCAVKTSRN PSPPDPLEPQ 150
    NTALAIASWG VDYIVLTSVD RDDLPDGGSG HFSETVQAMK KLKPEIMVEC 200
    LTSDFRGDLE AVETLVHSGL DVFAHNIETV KRLQRIVRDP RAGYEQSLSV 250
    LKHAKHSKEG MITKSSIMLG LGETDDELKE AMADLRAIDV DILTLGQYLQ 300
    PTPLHLTVKE YVTPEKFAFW KEYGESIGFR YVASGPMVRS SYRAGELFVK 350
    TMVKERSSNS AAKP 364
    Length:364
    Mass (Da):40,304
    Last modified:March 24, 2009 - v1
    Checksum:iC3B55B75770C5DA9
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ973826 Genomic DNA. Translation: EEF44086.1.
    RefSeqiXP_002518326.1. XM_002518280.1.

    Genome annotation databases

    GeneIDi8279195.
    KEGGircu:RCOM_0817880.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ973826 Genomic DNA. Translation: EEF44086.1 .
    RefSeqi XP_002518326.1. XM_002518280.1.

    3D structure databases

    ProteinModelPortali B9RX57.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8279195.
    KEGGi rcu:RCOM_0817880.

    Phylogenomic databases

    KOi K03644.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    MF_03129. Lipoyl_synth_plantC.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR027526. Lipoyl_synth_chlpt.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Hale.

    Entry informationi

    Entry nameiLISC_RICCO
    AccessioniPrimary (citable) accession number: B9RX57
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 33 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3