B9RW49 (LIAS_RICCO) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipoyl synthase, mitochondrial
EC=2.8.1.8

Alternative name(s):
Lipoate synthase
Short name=LS
Short name=Lip-syn
Lipoic acid synthase

Gene names

Name:	LIP1
ORF Names:	RCOM_1176060

Organism

Ricinus communis (Castor bean) [Complete proteome]

Taxonomic identifier

3988 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Malpighiales › Euphorbiaceae › Acalyphoideae › Acalypheae › Ricinus

Protein attributes

Sequence length	348 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives By similarity. HAMAP-Rule MF_03128
Catalytic activity	Protein N(6)-(octanoyl)lysine + 2 sulfur + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 L-methionine + 2 5'-deoxyadenosine. HAMAP-Rule MF_03128
Cofactor	Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Pathway	Protein modification; protein lipoylation via endogenous pathway; protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein]: step 2/2. HAMAP-Rule MF_03128
Subcellular location	Mitochondrion By similarity HAMAP-Rule MF_03128.
Sequence similarities	Belongs to the radical SAM superfamily. Lipoyl synthase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding S-adenosyl-L-methionine
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	protein lipoylation Inferred from electronic annotation. Source: HAMAP
Cellular_component	mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: HAMAP lipoate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 348		Lipoyl synthase, mitochondrial HAMAP-Rule MF_03128		PRO_0000398855
Sites
	Metal binding	105	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	110	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	116	1	Iron-sulfur 1 (4Fe-4S) By similarity
	Metal binding	136	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	140	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity
	Metal binding	143	1	Iron-sulfur 2 (4Fe-4S-S-AdoMet) By similarity

Sequences

	Sequence		Length	Mass (Da)
	B9RW49 [UniParc]. Last modified March 24, 2009. Version 1. Checksum: 05CAF5FBB2772DA3	FASTA	348	38,584
	10 20 30 40 50 60 MFQSRFTILI RTLNSSKSRH FSSTIEPTKP QFPQTLAGLR ARLAAESPSL SEFSDLQSNN 70 80 90 100 110 120 SYSVEVGTKK KPLPKPKWMR EAIPGGDKYV QIKKKLRELK LHTVCEEAKC PNLGECWSGG 130 140 150 160 170 180 ETGTATATIM ILGDTCTRGC RFCNVKTSRT PPPPDPDEPA NVAEAIASWG LDYVVITSVD 190 200 210 220 230 240 RDDLPDQGSN HFAQTVQKLK ALKPHMLIEA LVPDFRGDPG CVENVAKSGL DVFAHNIETV 250 260 270 280 290 300 EDLQSVIRDH RANFKQSLDV LMMAKDHAPK GTLTKTSIML GCGETPEQVV KTMEKVRAAG 310 320 330 340 VDVMTFGQYM RPSKRHMPVS EYVTPEAFEQ YRTXXLVSYV LFSLLISV

References

[1]

"Draft genome sequence of the oilseed species Ricinus communis." Chan A.P., Crabtree J., Zhao Q., Lorenzi H., Orvis J., Puiu D., Melake-Berhan A., Jones K.M., Redman J., Chen G., Cahoon E.B., Gedil M., Stanke M., Haas B.J., Wortman J.R., Fraser-Liggett C.M., Ravel J., Rabinowicz P.D. Nat. Biotechnol. 28:951-956(2010) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Hale.

Cross-references

Sequence databases
EMBL GenBank DDBJ	EQ973822 Genomic DNA. Translation: EEF44486.1.
RefSeq	XP_002517968.1. XM_002517922.1.
3D structure databases
ProteinModelPortal	B9RW49.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	8262116.
KEGG	rcu:RCOM_1176060.
Phylogenomic databases
KO	K03644.
ProtClustDB	PLN02428.
Enzyme and pathway databases
UniPathway	UPA00538; UER00593.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_03128. Lipoyl_synth_plantM. Divergent sequence.
InterPro	IPR013785. Aldolase_TIM. IPR006638. Elp3/MiaB/NifB. IPR003698. Lipoyl_synth. IPR007197. rSAM. [Graphical view]
PANTHER	PTHR10949. PTHR10949. 1 hit.
Pfam	PF04055. Radical_SAM. 1 hit. [Graphical view]
PIRSF	PIRSF005963. Lipoyl_synth. 1 hit.
SMART	SM00729. Elp3. 1 hit. [Graphical view]
TIGRFAMs	TIGR00510. lipA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LIAS_RICCO

Accession

Primary (citable) accession number: B9RW49

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 5, 2010
Last sequence update:	March 24, 2009
Last modified:	May 29, 2013
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program