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B9RW49

- LIAS_RICCO

UniProt

B9RW49 - LIAS_RICCO

Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Ricinus communis (Castor bean)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 32 (01 Oct 2014)
      Sequence version 1 (24 Mar 2009)
      Previous versions | rss
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    Functioni

    Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.By similarity

    Catalytic activityi

    Protein N(6)-(octanoyl)lysine + 2 sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = protein N(6)-(lipoyl)lysine + 2 (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.

    Cofactori

    Binds 2 4Fe-4S clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi105 – 1051Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi110 – 1101Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi116 – 1161Iron-sulfur 1 (4Fe-4S)By similarity
    Metal bindingi136 – 1361Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi140 – 1401Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity
    Metal bindingi143 – 1431Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. lipoate synthase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00538; UER00593.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Lipoyl synthase, mitochondrial (EC:2.8.1.8)
    Alternative name(s):
    Lipoate synthase
    Short name:
    LS
    Short name:
    Lip-syn
    Lipoic acid synthase
    Gene namesi
    Name:LIP1
    ORF Names:RCOM_1176060
    OrganismiRicinus communis (Castor bean)
    Taxonomic identifieri3988 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus
    ProteomesiUP000008311: Unassembled WGS sequence

    Subcellular locationi

    Mitochondrion By similarity

    GO - Cellular componenti

    1. mitochondrion Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 348Lipoyl synthase, mitochondrialPRO_0000398855
    Transit peptidei1 – ?MitochondrionSequence Analysis

    Structurei

    3D structure databases

    ProteinModelPortaliB9RW49.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    KOiK03644.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    HAMAPiMF_00206. Lipoyl_synth.
    InterProiIPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view]
    PANTHERiPTHR10949. PTHR10949. 1 hit.
    PfamiPF04055. Radical_SAM. 1 hit.
    [Graphical view]
    PIRSFiPIRSF005963. Lipoyl_synth. 1 hit.
    SMARTiSM00729. Elp3. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR00510. lipA. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    B9RW49-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFQSRFTILI RTLNSSKSRH FSSTIEPTKP QFPQTLAGLR ARLAAESPSL    50
    SEFSDLQSNN SYSVEVGTKK KPLPKPKWMR EAIPGGDKYV QIKKKLRELK 100
    LHTVCEEAKC PNLGECWSGG ETGTATATIM ILGDTCTRGC RFCNVKTSRT 150
    PPPPDPDEPA NVAEAIASWG LDYVVITSVD RDDLPDQGSN HFAQTVQKLK 200
    ALKPHMLIEA LVPDFRGDPG CVENVAKSGL DVFAHNIETV EDLQSVIRDH 250
    RANFKQSLDV LMMAKDHAPK GTLTKTSIML GCGETPEQVV KTMEKVRAAG 300
    VDVMTFGQYM RPSKRHMPVS EYVTPEAFEQ YRTXXLVSYV LFSLLISV 348
    Length:348
    Mass (Da):38,584
    Last modified:March 24, 2009 - v1
    Checksum:i05CAF5FBB2772DA3
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ973822 Genomic DNA. Translation: EEF44486.1.
    RefSeqiXP_002517968.1. XM_002517922.1.

    Genome annotation databases

    GeneIDi8262116.
    KEGGircu:RCOM_1176060.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    EQ973822 Genomic DNA. Translation: EEF44486.1 .
    RefSeqi XP_002517968.1. XM_002517922.1.

    3D structure databases

    ProteinModelPortali B9RW49.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 8262116.
    KEGGi rcu:RCOM_1176060.

    Phylogenomic databases

    KOi K03644.

    Enzyme and pathway databases

    UniPathwayi UPA00538 ; UER00593 .

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    HAMAPi MF_00206. Lipoyl_synth.
    InterProi IPR013785. Aldolase_TIM.
    IPR006638. Elp3/MiaB/NifB.
    IPR003698. Lipoyl_synth.
    IPR007197. rSAM.
    [Graphical view ]
    PANTHERi PTHR10949. PTHR10949. 1 hit.
    Pfami PF04055. Radical_SAM. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF005963. Lipoyl_synth. 1 hit.
    SMARTi SM00729. Elp3. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR00510. lipA. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Hale.

    Entry informationi

    Entry nameiLIAS_RICCO
    AccessioniPrimary (citable) accession number: B9RW49
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 5, 2010
    Last sequence update: March 24, 2009
    Last modified: October 1, 2014
    This is version 32 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3