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Protein

Lipoyl synthase, mitochondrial

Gene

LIP1

Organism
Ricinus communis (Castor bean)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives.By similarity

Catalytic activityi

Protein N6-(octanoyl)lysine + an [Fe-S] cluster scaffold protein carrying a [4Fe-4S]2+ cluster + 2 S-adenosyl-L-methionine + 2 oxidized [2Fe-2S] ferredoxin + 6 H+ = protein N6-(dihydrolipoyl)lysine + an [Fe-S] cluster scaffold protein + 2 sulfide + 4 Fe3+ + 2 L-methionine + 2 5'-deoxyadenosine + 2 reduced [2Fe-2S] ferredoxin.

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 2 [4Fe-4S] clusters per subunit. One cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.By similarity

Pathwayi: protein lipoylation via endogenous pathway

This protein is involved in step 2 of the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein].
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Lipoyl synthase, chloroplastic (LIP1P), Lipoyl synthase, mitochondrial (LIP1)
This subpathway is part of the pathway protein lipoylation via endogenous pathway, which is itself part of Protein modification.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes protein N(6)-(lipoyl)lysine from octanoyl-[acyl-carrier-protein], the pathway protein lipoylation via endogenous pathway and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi105Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi110Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi116Iron-sulfur 1 (4Fe-4S)By similarity1
Metal bindingi136Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi140Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity1
Metal bindingi143Iron-sulfur 2 (4Fe-4S-S-AdoMet)By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionTransferase
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

UniPathwayiUPA00538; UER00593

Names & Taxonomyi

Protein namesi
Recommended name:
Lipoyl synthase, mitochondrial (EC:2.8.1.8)
Alternative name(s):
Lipoate synthase
Short name:
LS
Short name:
Lip-syn
Lipoic acid synthase
Gene namesi
Name:LIP1
ORF Names:RCOM_1176060
OrganismiRicinus communis (Castor bean)
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus
Proteomesi
  • UP000008311 Componenti: Unassembled WGS sequence

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000398855? – 348Lipoyl synthase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Structurei

3D structure databases

ProteinModelPortaliB9RW49
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

InParanoidiB9RW49

Family and domain databases

Gene3Di3.20.20.70, 1 hit
HAMAPiMF_00206 Lipoyl_synth, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR006638 Elp3/MiaB/NifB
IPR031691 LIAS_N
IPR003698 Lipoyl_synth
IPR007197 rSAM
PfamiView protein in Pfam
PF16881 LIAS_N, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF005963 Lipoyl_synth, 1 hit
SFLDiSFLDG01058 lipoyl_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00510 lipA, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

B9RW49-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFQSRFTILI RTLNSSKSRH FSSTIEPTKP QFPQTLAGLR ARLAAESPSL
60 70 80 90 100
SEFSDLQSNN SYSVEVGTKK KPLPKPKWMR EAIPGGDKYV QIKKKLRELK
110 120 130 140 150
LHTVCEEAKC PNLGECWSGG ETGTATATIM ILGDTCTRGC RFCNVKTSRT
160 170 180 190 200
PPPPDPDEPA NVAEAIASWG LDYVVITSVD RDDLPDQGSN HFAQTVQKLK
210 220 230 240 250
ALKPHMLIEA LVPDFRGDPG CVENVAKSGL DVFAHNIETV EDLQSVIRDH
260 270 280 290 300
RANFKQSLDV LMMAKDHAPK GTLTKTSIML GCGETPEQVV KTMEKVRAAG
310 320 330 340
VDVMTFGQYM RPSKRHMPVS EYVTPEAFEQ YRTXXLVSYV LFSLLISV
Length:348
Mass (Da):38,584
Last modified:March 24, 2009 - v1
Checksum:i05CAF5FBB2772DA3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ973822 Genomic DNA Translation: EEF44486.1

Similar proteinsi

Entry informationi

Entry nameiLIAS_RICCO
AccessioniPrimary (citable) accession number: B9RW49
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 5, 2010
Last sequence update: March 24, 2009
Last modified: May 23, 2018
This is version 44 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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