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Protein

ATP-dependent 6-phosphofructokinase

Gene

PFK

Organism
Ricinus communis (Castor bean)
Status
Unreviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.UniRule annotation

Catalytic activityi

ATP + D-fructose 6-phosphate = ADP + D-fructose 1,6-bisphosphate.UniRule annotation

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Allosterically activated by AMP.UniRule annotation

Pathway:iglycolysis

This protein is involved in step 3 of the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Glucose-6-phosphate isomerase (RCOM_1594100), Glucose-6-phosphate isomerase (RCOM_1039110)
  3. ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit beta (PFP-BETA), ATP-dependent 6-phosphofructokinase (PFK), Pyrophosphate--fructose 6-phosphate 1-phosphotransferase subunit alpha (PFP-ALPHA), ATP-dependent 6-phosphofructokinase (PFK), ATP-dependent 6-phosphofructokinase (PFK)
  4. Fructose-bisphosphate aldolase (RCOM_0577600), Fructose-bisphosphate aldolase (RCOM_0383870), Fructose-bisphosphate aldolase (RCOM_0291680), Fructose-bisphosphate aldolase (RCOM_1180710), Fructose-bisphosphate aldolase (RCOM_1450320), Fructose-bisphosphate aldolase (RCOM_1538490)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-glyceraldehyde 3-phosphate and glycerone phosphate from D-glucose, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei105 – 1051ATP; via amide nitrogenUniRule annotation
Metal bindingi194 – 1941Magnesium; catalyticUniRule annotation
Sitei195 – 1951Important for substrate specificity; cannot use PPi as phosphoryl donorUniRule annotation
Active sitei224 – 2241Proton acceptorUniRule annotation
Binding sitei323 – 3231SubstrateUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi168 – 1692ATPUniRule annotation
Nucleotide bindingi193 – 1964ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

KinaseUniRule annotationImported, Transferase

Keywords - Biological processi

GlycolysisUniRule annotation

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent 6-phosphofructokinaseUniRule annotation (EC:2.7.1.11UniRule annotation)
Short name:
ATP-PFKUniRule annotation
Short name:
PhosphofructokinaseUniRule annotation
Alternative name(s):
PhosphohexokinaseUniRule annotation
Gene namesi
Name:PFKUniRule annotation
ORF Names:RCOM_0799050Imported
OrganismiRicinus communis (Castor bean)Imported
Taxonomic identifieri3988 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsMalpighialesEuphorbiaceaeAcalyphoideaeAcalypheaeRicinus
ProteomesiUP000008311 Componenti: Unassembled WGS sequence

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

CytoplasmUniRule annotation

Interactioni

Subunit structurei

Homotetramer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliB9RRX6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni222 – 2243Substrate bindingUniRule annotation
Regioni267 – 2693Substrate bindingUniRule annotation
Regioni380 – 3834Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the phosphofructokinase type A (PFKA) family. PPi-dependent PFK group II subfamily. Atypical ATP-dependent clade "X" sub-subfamily.UniRule annotation

Phylogenomic databases

InParanoidiB9RRX6.
KOiK00850.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.

Sequencei

Sequence statusi: Complete.

B9RRX6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGNADGNLQS QMMKIVNGEA GYLLEDVPHL TDYIPDLPTY PNPLQDNPAY
60 70 80 90 100
SAVKQYFVNV DDTVAQKIVV HKDGPRGTHF RRAGPRQKVY FKSDEVHACI
110 120 130 140 150
VTCGGLCPGL NTVIREIVCG LHHMYGVTKV LGIDGGYRGF YSKNTVALTP
160 170 180 190 200
KVVNDIHKRG GTILGTSRGG HDKSKIVDSI QDRGINQVYI IGGDGTQKGA
210 220 230 240 250
AVIYEEIQRR GLKVAVAGIP KTIDNDIPVI DKSFGFDSAV EEAQRAINAA
260 270 280 290 300
HVEAESTENG IGVVKLMGRY SGFIAMYATL ASRDVDCCLI PESPFYLEGQ
310 320 330 340 350
GGLLEFIGKR LKENGHMVIV IAEGAGQDLV SKSLQSMNQQ DASGNKLLQD
360 370 380 390 400
VGLWISQRIK DHFAREHRMN ITLKYIDPTY MIRAIPSNAS DNVYCTLLAH
410 420 430 440 450
SAVHGAMAGY TGFTVGPVNG RHAYIPFNRI TEKQNNVVIT DRMWARLLSS
460 470 480
TDQPSFMSPK VLTKVKKQED PPTKLDGDNL KDGNQ
Length:485
Mass (Da):53,104
Last modified:March 24, 2009 - v1
Checksum:i65BDC31936D0F7AD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ973807 Genomic DNA. Translation: EEF45836.1.
RefSeqiXP_002516495.1. XM_002516449.1.

Genome annotation databases

GeneIDi8275648.
KEGGircu:RCOM_0799050.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
EQ973807 Genomic DNA. Translation: EEF45836.1.
RefSeqiXP_002516495.1. XM_002516449.1.

3D structure databases

ProteinModelPortaliB9RRX6.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi8275648.
KEGGircu:RCOM_0799050.

Phylogenomic databases

InParanoidiB9RRX6.
KOiK00850.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00182.

Family and domain databases

HAMAPiMF_01981. Phosphofructokinase_II_X.
InterProiIPR022953. ATP_PFK.
IPR000023. Phosphofructokinase_dom.
IPR012004. PyroP-dep_PFK_TP0108.
[Graphical view]
PfamiPF00365. PFK. 1 hit.
[Graphical view]
PIRSFiPIRSF000534. PPi_PFK_TP0108. 1 hit.
PRINTSiPR00476. PHFRCTKINASE.
SUPFAMiSSF53784. SSF53784. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. HaleImported.

Entry informationi

Entry nameiB9RRX6_RICCO
AccessioniPrimary (citable) accession number: B9RRX6
Entry historyi
Integrated into UniProtKB/TrEMBL: March 24, 2009
Last sequence update: March 24, 2009
Last modified: May 27, 2015
This is version 33 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Allosteric enzymeUniRule annotation, Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.